Comparison of enzyme immunoassays and rapid diagnostic tests for clostridium difficile glutamate dehydrogenase and toxin a + B to toxinogenic culture on a highly selective chromogenic medium

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 35, Issue 10, 2016, Pages 1655-1659

  Olling, A ^a   Leidinger, H ^a   Hoffmann, R ^b  

^a R Biopharm AG Rhome Ltd Scotland   (Germany)

^b KLINIKUM AUGSBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CLOSTRIDIUM DIFFICILE TOXIN A; CLOSTRIDIUM DIFFICILE TOXIN B; GLUTAMATE DEHYDROGENASE; RNA 16S; BACTERIAL TOXIN; CULTURE MEDIUM;

AGAR MEDIUM; ARTICLE; BACTERIAL COLONIZATION; BACTERIUM CULTURE; CLOSTRIDIUM DIFFICILE INFECTION; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME IMMUNOASSAY; FECES; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RAPID TEST; SAMPLE; TOXIN SYNTHESIS; TOXINOGENIC CULTURE; CHEMISTRY; CLOSTRIDIUM INFECTIONS; COMPARATIVE STUDY; DIAGNOSTIC TEST; DIARRHEA; EVALUATION STUDY; GROWTH, DEVELOPMENT AND AGING; HUMAN; IMMUNOAFFINITY CHROMATOGRAPHY; ISOLATION AND PURIFICATION; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; PEPTOCLOSTRIDIUM DIFFICILE; PROCEDURES;

BACTERIAL TOXINS; BACTERIOLOGICAL TECHNIQUES; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; CULTURE MEDIA; DIAGNOSTIC TESTS, ROUTINE; DIARRHEA; GLUTAMATE DEHYDROGENASE; HUMANS; IMMUNOCHROMATOGRAPHY; IMMUNOENZYME TECHNIQUES;

EID: 84975321362     PISSN: 09349723     EISSN: 14354373     Source Type: Journal    
DOI: 10.1007/s10096-016-2706-9     Document Type: Article

References (16)

1. Cohen SH, Gerding DN, Johnson S, Kelly CP, Loo VG, McDonald LC, Pepin J, Wilcox MH, Society for Healthcare Epidemiology of America, Infectious Diseases Society of America (2010) Clinical practice guidelines for Clostridium difficile infection in adults: 2010 update by the Society for Healthcare Epidemiology of America (SHEA) and the Infectious Diseases Society of America (IDSA). Infect Control Hosp Epidemiol 31:431–455
2. Crobach MJT, Dekkers OM, Wilcox MH, Kuijper EJ (2009) European Society of Clinical Microbiology and Infectious Diseases (ESCMID): data review and recommendations for diagnosing Clostridium difficile-infection (CDI). Clin Microbiol Infect 15:1053–1066
3. Fenner L, Widmer AF, Goy G, Rudin S, Frei R (2008) Rapid and reliable diagnostic algorithm for detection of Clostridium difficile. J Clin Microbiol 46:328–330
4. Reller ME, Lema CA, Perl TM, Cai M, Ross TL, Speck KA, Carroll KC (2007) Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for detection of toxigenic Clostridium difficile. J Clin Microbiol 45:3601–3605
5. Reyes RC, John MA, Ayotte DL, Covacich A, Milburn S, Hussain Z (2007) Performance of TechLab C. DIFF QUIK CHEK and TechLab C. DIFFICILE TOX A/B II for the detection of Clostridium difficile in stool samples. Diagn Microbiol Infect Dis 59:33–37
6. Snell H, Ramos M, Longo S, John M, Hussain Z (2004) Performance of the TechLab C. DIFF CHEK-60 enzyme immunoassay (EIA) in combination with the C. difficile Tox A/B II EIA kit, the Triage C. difficile panel immunoassay, and a cytotoxin assay for diagnosis of Clostridium difficile-associated diarrhea. J Clin Microbiol 42:4863–4865
7. Carson KC, Boseiwaqa LV, Thean SK, Foster NF, Riley TV (2013) Isolation of Clostridium difficile from faecal specimens—a comparison of chromID C. difficile agar and cycloserine-cefoxitin-fructose agar. J Med Microbiol 62:1423–1427
8. Boseiwaqa LV, Foster NF, Thean SK, Squire MM, Riley TV, Carson KC (2013) Comparison of ChromID C. difficile agar and cycloserine–cefoxitin–fructose agar for the recovery of Clostridium difficile. Pathology (Phila) 45:495–500
9. Davies KA, Berry CE, Morris KA, Smith R, Young S, Davis TE, Fuller DD, Buckner RJ, Wilcox MH (2015) Comparison of the VIDAS C. difficile GDH automated enzyme-linked fluorescence immunoassay (ELFA) with another commercial enzyme immunoassay (EIA) (Quik Chek-60), two selective media, and a PCR assay for gluD for detection of Clostridium difficile in fecal samples. J Clin Microbiol 53:1931–1934
10. Van den Berg RJ, Kuijper EJ, van Coppenraet LE, Claas EC (2006) Rapid diagnosis of toxigenic Clostridium difficile in faecal samples with internally controlled real-time PCR. Clin Microbiol Infect 12:184–186
11. Polage CR, Gyorke CE, Kennedy MA, Leslie JL, Chin DL, Wang S, Nguyen HH, Huang B, Tang YW, Lee LW, Kim K, Taylor S, Romano PS, Panacek EA, Goodell PB, Solnick JV, Cohen SH (2015) Overdiagnosis of Clostridium difficile infection in the molecular test era. JAMA Intern Med 175:1792–1801
12. Burnham CA, Carroll KC (2013) Diagnosis of Clostridium difficile infection: an ongoing conundrum for clinicians and for clinical laboratories. Clin Microbiol Rev 26:604–630
13. Karpiński P, Pituch H, Lachowicz D, Piotrowski M, Obuch-Woszczatyński P (2015) Evaluation of growth of clinical Clostridium difficile strains belonging to different PCR-ribotypes on chromID C. difficile Agar. Med Dosw Mikrobiol 67:9–14
14. Issack MI, Elliott TS (1990) Clostridium difficile carriage after infection. Lancet 335:610–611
15. Planche TD, Davies KA, Coen PG, Finney JM, Monahan IM, Morris KA, O’Connor L, Oakley SJ, Pope CF, Wren MW, Shetty NP, Crook DW, Wilcox MH (2013) Differences in outcome according to Clostridium difficile testing method: a prospective multicentre diagnostic validation study of C difficile infection. Lancet Infect Dis 13:936–945
16. Perry JD, Asir K, Halimi D, Orenga S, Dale J, Payne M, Carlton R, Evans J, Gould FK (2010) Evaluation of a chromogenic culture medium for isolation of Clostridium difficile within 24 hours. J Clin Microbiol 48:3852–3858