Evaluation of a chromogenic culture medium for isolation of Clostridium difficile within 24 hours

Journal of Clinical Microbiology

Volumn 48, Issue 11, 2010, Pages 3852-3858

  Perry, John D ^a   Asir, Kerry ^a   Halimi, Diane ^b   Orenga, Sylvain ^b   Dale, Joanne ^a   Payne, Michelle ^a   Carlton, Ruth ^a   Evans, Jim ^c   Gould, F Kate ^a  

^a FREEMAN HOSPITAL   (United Kingdom)

^b BIOMÉRIEUX   (France)

^c PUBLIC HEALTH ENGLAND   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL;

AGAR MEDIUM; ARTICLE; BACTERIAL COUNT; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM ISOLATE; BACTERIUM ISOLATION; CLOSTRIDIUM DIFFICILE; CONTROLLED STUDY; CULTURE MEDIUM; FECES ANALYSIS; INOCULATION; NONHUMAN; PRIORITY JOURNAL; SHOCK; SUSPENSION;

BACTERIOLOGICAL TECHNIQUES; CHROMOGENIC COMPOUNDS; CLOSTRIDIUM DIFFICILE; CULTURE MEDIA; ENTEROCOLITIS, PSEUDOMEMBRANOUS; HUMANS; TIME FACTORS;

CLOSTRIDIUM DIFFICILE;

EID: 78049516561     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01288-10     Document Type: Article

References (30)

1. Aspinall, S. T., and D. N. Hutchinson. 1992. New selective medium for isolating Clostridium difficile from faeces. J. Clin. Pathol. 45:812-814.
2. Bartley, S. L., and V. R. Dowell, Jr. 1991. Comparison of media for the isolation of Clostridium difficile from fecal specimens. Lab. Med. 22:335-338.
3. Bliss, D. Z., S. Johnson, C. R. Clabots, K. Savik, and D. N. Gerding. 1997. Comparison of cycloserine-cefoxitin-fructose agar (CCFA) and taurocholate-CCFA for recovery of Clostridium difficile during surveillance of hospitalized patients. Diagn. Microbiol. Infect. Dis. 29:1-4.
4. Bloedt, K., M. Riecker, S. Poppert, and N. Wellinghausen. 2009. Evaluation of new selective culture media and a rapid fluorescence in situ hybridization assay for identification of Clostridium difficile from stool samples. J. Med. Microbiol. 58:874-877.
5. Brazier, J. S. 1998. The diagnosis of Clostridium difficile-associated disease. J. Antimicrob. Chemother. 41(Suppl. C):29-40.
6. Brazier, J. S., and B. I. Duerden. 1998. Guidelines for optimal surveillance of Clostridium difficile infection in hospitals. Commun. Dis. Public Health 1:229-230.
7. Crobach, M. J., O. M. Dekkers, M. H. Wilcox, and E. J. Kuijper. 2009. European Society of Clinical Microbiology and Infectious Diseases (ESCMID): data review and recommendations for diagnosing Clostridium difficile-infection (CDI). Clin. Microbiol. Infect. 15:1053-1066.
8. Delmée, M., J. Van Broeck, A. Simon, M. Janssens, and V. Avesani. 2005. Laboratory diagnosis of Clostridium difficile-associated diarrhoea: a plea for culture. J. Med. Microbiol. 54:187-191.
9. Eastwood, K., P. Else, A. Charlett, and M. Wilcox. 2009. Comparison of nine commercially available Clostridium difficile toxin detection assays, a real-time PCR assay for C. difficile tcdB, and a glutamate dehydrogenase detection assay to cytotoxin testing and cytotoxigenic culture methods. J. Clin. Microbiol. 47:3211-3217.
10. Fenner, L., A. F. Widmer, G. Goy, S. Rudin, and R. Frei. 2008. Rapid and reliable diagnostic algorithm for detection of Clostridium difficile. J. Clin. Microbiol. 46:328-330.
11. García, A., J. L. Pérez, A. Pulido, J. Niubó, P. Pérez, and R. Martín. 2000. Evaluation of four rapid methods for the investigation of the toxigenic capacity of Clostridium difficile strains isolated in a selective medium. Enferm. Infecc. Microbiol. Clin. 18:109-112.
12. George, W. L., V. L. Sutter, D. Citron, and S. M. Finegold. 1979. Selective and differential medium for isolation of Clostridium difficile. J. Clin. Microbiol. 9:214-219.
13. Gerding, D. N. 2007. New definitions will help, but cultures are critical for resolving unanswered questions about Clostridium difficile. Infect. Control Hosp. Epidemiol. 28:113-115.
14. Goorhuis, A., D. Bakker, J. Corver, S. B. Debast, C. Harmanus, D. W. Notermans, A. A. Bergwerff, F. W. Dekker, and E. J. Kuijper. 2008. Emergence of Clostridium difficile infection due to a new hypervirulent strain, polymerase chain reaction ribotype 078. Clin. Infect. Dis. 47:1162-1170.
15. Jaksic, A. S., G. R. Nimmo, and B. W. Dwyer. 2009. Laboratory diagnosis of Clostridium difficile-associated diarrhoea: microbiologists (should) do it with culture. Pathology 41:187-188.
16. Johal, S. S., J. Hammond, K. Solomon, P. D. James, and Y. R. Mahida. 2004. Clostridium difficile associated diarrhoea in hospitalised patients: onset in the community and hospital and role of flexible sigmoidoscopy. Gut 53:673-677.
17. Kuijper, E. J., F. Barbut, J. S. Brazier, N. Kleinkauf, T. Eckmanns, M. L. Lambert, D. Drudy, F. Fitzpatrick, C. Wiuff, D. J. Brown, J. E. Coia, H. Pituch, P. Reichert, J. Even, J. Mossong, A. F. Widmer, K. E. Olsen, F. Allerberger, D. W. Notermans, M. Delmée, B. Coignard, M. Wilcox, B. Patel, R. Frei, E. Nagy, E. Bouza, M. Marin, T. Akerlund, A. Virolainen-Julkunen, O. Lyytikäinen, S. Kotila, A. Ingebretsen, B. Smyth, P. Rooney, I. R. Poxton, and D. L. Monnet. 2008. Update of Clostridium difficile infection due to PCR ribotype 027 in Europe, 2008. Euro Surveill. 13(31):pii=18942.
18. Levett, P. N. 1985. Effect of antibiotic concentration in a selective medium on the isolation of Clostridium difficile from faecal specimens. J. Clin. Pathol. 38:233-234.
19. Marler, L. M., J. A. Siders, L. C. Wolters, Y. Pettigrew, B. L. Skitt, and S. D. Allen. 1992. Comparison of five cultural procedures for isolation of Clostridium difficile from stools. J. Clin. Microbiol. 30:514-516.
20. Mundy, L. S., C. J. Shanholtzer, K. E. Willard, D. N. Gerding, and L. R. Peterson. 1995. Laboratory detection of Clostridium difficile. A comparison of media and incubation systems. Am. J. Clin. Pathol. 103:52-56.
21. Perry, J. D., and A. M. Freydière. 2007. The application of chromogenic media in clinical microbiology. J. Appl. Microbiol. 103:2046-2055.
22. Reller, M. E., C. A. Lema, T. M. Perl, M. Cai, T. L. Ross, K. A. Speck, and K. C. Carroll. 2007. Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for detection of toxigenic Clostridium difficile. J. Clin. Microbiol. 45:3601-3605.
23. Riley, T. V., J. S. Brazier, H. Hassan, K. Williams, and K. D. Phillips. 1987. Comparison of alcohol shock enrichment and selective enrichment for the isolation of Clostridium difficile. Epidemiol. Infect. 99:355-359.
24. Rousseau, C., I. Poilane, F. Diakite, L. Feghoul, P. Cruaud, and A. Collignon. 2010. Comparison of three Clostridium difficile culture media: interest of enhancing spore germination media? Pathol. Biol. 58:58-61.
25. Sharp, S. E., W. M. Ivie, M. R. Buckles, D. M. Coover, J. C. Pohl, and P. A. Hatcher. 2009. A simple 3-step algorithm for improved laboratory detection of Clostridium difficile toxin without the need for tissue culture cytotoxicity neutralization assays. Diagn. Microbiol. Infect. Dis. 64:344-346.
26. Shin, B. M., E. Y. Kuak, E. J. Lee, and J. G. Songer. 2009. Algorithm combining toxin immunoassay and stool culture for diagnosis of Clostridium difficile infection. J. Clin. Microbiol. 47:2952-2956.
27. Staneck, J. L., L. S. Weckbach, S. D. Allen, J. A. Siders, P. H. Gilligan, G. Coppitt, J. A. Kraft, and D. H. Willis. 1996. Multicenter evaluation of four methods for Clostridium difficile detection: ImmunoCard C. difficile, cytotoxin assay, culture, and latex agglutination. J. Clin. Microbiol. 34:2718-2721.
28. Stubbs, S. L., J. S. Brazier, G. L. O'Neill, and B. I. Duerden. 1999. PCR targeted to the 16S-23S rRNA gene intergenic spacer region of Clostridium difficile and construction of a library consisting of 116 different PCR ribotypes. J. Clin. Microbiol. 37:461-463.
29. Thonnard, J., F. Carreer, V. Avesani, and M. Delmée. 1996. Toxin A detection on Clostridium difficile colonies from 24-h cultures. Clin. Microbiol. Infect. 2:50-54.
30. Wilcox, M. H., W. N. Fawley, and P. Parnell. 2000. Value of lysozyme agar incorporation and alkaline thioglycollate exposure for the environmental recovery of Clostridium difficile. J. Hosp. Infect. 44:65-69.