Rapid and reliable diagnostic algorithm for detection of Clostridium difficile

Journal of Clinical Microbiology

Volumn 46, Issue 1, 2008, Pages 328-330

  Fenner, Lukas ^a   Widmer, Andreas F ^a   Goy, Gisela ^a   Rudin, Sonja ^a   Frei, Reno ^a  

^a UNIVERSITY HOSPITAL BASEL   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ANTIGEN; CLOSTRIDIUM DIFFICILE TOXIN A; CLOSTRIDIUM DIFFICILE TOXIN B; GLUTAMATE DEHYDROGENASE;

ADULT; ALGORITHM; ARTICLE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CLOSTRIDIUM DIFFICILE; EVALUATION; FECES; FECES CULTURE; HUMAN; IMMUNOASSAY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RELIABILITY; SCREENING; SENSITIVITY AND SPECIFICITY;

ADULT; ALGORITHMS; ANTIGENS, BACTERIAL; BACTERIAL TOXINS; BACTERIOLOGICAL TECHNIQUES; CLOSTRIDIUM DIFFICILE; ENTEROCOLITIS, PSEUDOMEMBRANOUS; ENTEROTOXINS; FECES; GLUTAMATE DEHYDROGENASE; HUMANS; IMMUNOASSAY;

CLOSTRIDIUM DIFFICILE;

EID: 38149108080     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.01503-07     Document Type: Article

References (13)

1. Asha, N. J., D. Tompkins, and M. H. Wilcox. 2006. Comparative analysis of prevalence, risk factors, and molecular epidemiology of antibiotic-associated diarrhea due to Clostridium difficile, Clostridium perfringens, and Staphylococcus aureus. J. Clin. Microbiol. 44:2785-2791.
2. Barbut, F., M. Delmee, J. S. Brazier, J. C. Petit, I. R. Poxton, M. Rupnik, V. Lalande, C. Schneider, P. Mastrantonio, R. Alonso, E. Kuipjer, and M. Tvede. 2003. A European survey of diagnostic methods and testing protocols for Clostridium difficile. Clin. Microbiol. Infect. 9:989-996.
3. Delmee, M., B. J. Van, A. Simon, M. Janssens, and V. Avesani. 2005. Laboratory diagnosis of Clostridium difficile-associated diarrhoea: a plea for culture. J. Med. Microbiol. 54:187-191.
4. Kelly, C. P., and J. T. LaMont. 1998. Clostridium difficile infection. Annu. Rev. Med. 49:375-390.
5. Kuijper, E. J., B. Coignard, and P. Tull. 2006. Emergence of Clostridium difficile-associated disease in North America and Europe. Clin. Microbiol. Infect. 12(Suppl. 6):2-18.
6. McDonald, L. C., G. E. Killgore, A. Thompson, R. C. Owens, Jr., S. V. Kazakova, S. P. Sambol, S. Johnson, and D. N. Gerding. 2005. An epidemic, toxin gene-variant strain of Clostridium difficile. N. Engl. J. Med. 353:2433-2441.
7. O'Connor, D., P. Hynes, M. Cormican, E. Collins, G. Corbett-Feeney, and M. Cassidy. 2001. Evaluation of methods for detection of toxins in specimens of feces submitted for diagnosis of Clostridium difficile-associated diarrhea. J. Clin. Microbiol. 39:2846-2849.
8. Reller, M. E., C. A. Lema, T. M. Perl, M. Cai, T. L. Ross, K. A. Speck, and K. C. Carroll. 2007. Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for the detection of toxigenic Clostridium difficile. J. Clin. Microbiol. 45:3601-3605.
9. Snell, H., M. Ramos, S. Longo, M. John, and Z. Hussain. 2004. Performance of the TechLab C. DIFF CHEK-60 enzyme immunoassay (EIA) in combination with the C. difficile Tox A/B II EIA kit, the Triage C. difficile panel immunoassay, and a cytotoxin assay for diagnosis of Clostridium difficile-associated diarrhea. J. Clin. Microbiol. 42:4863-4865.
10. Ticehurst, J. R., D. Z. Aird, L. M. Dam, A. P. Borek, J. T. Hargrove, and K. C. Carroll. 2006. Effective detection of toxigenic Clostridium difficile by a two-step algorithm including tests for antigen and cytotoxin. J. Clin. Microbiol. 44:1145-1149.
11. Turgeon, D. K., T. J. Novicki, J. Quick, L. Carlson, P. Miller, B. Ulness, A. Cent, R. Ashley, A. Larson, M. Coyle, A. P. Limaye, B. T. Cookson, and T. R. Fritsche. 2003. Six rapid tests for direct detection of Clostridium difficile and its toxins in fecal samples compared with the fibroblast cytotoxicity assay. J. Clin. Microbiol. 41:667-670.
12. van den Berg, R. J., L. S. Bruijnesteijn van Coppenraet, H.-J. Gerritsen, H. P. Endtz, E. R. van der Vorm, and E. J. Kuijper. 2005. Prospective multicenter evaluation of a new immunoassay and real-time PCR for rapid diagnosis of Clostridium difficile-associated diarrhea in hospitalized patients. J. Clin. Microbiol. 43:5338-5340.
13. Zheng, L., S. F. Keller, D. M. Lyerly, R. J. Carman, C. W. Genheimer, C. A. Gleaves, S. J. Kohlhepp, S. Young, S. Perez, and K. Ye. 2004. Multicenter evaluation of a new screening test that detects Clostridium difficile in fecal specimens. J. Clin. Microbiol. 42:3837-3840.