IBH3: Simple, fixable BH3 profiling to determine apoptotic priming in primary tissue by flow cytometry

Biological Chemistry

Volumn 397, Issue 7, 2016, Pages 671-678

  Ryan, Jeremy ^a   Montero, Joan ^a   Rocco, James ^b   Letai, Anthony ^a  

^a DANA FARBER CANCER INSTITUTE   (United States)

^b THE OHIO STATE UNIVERSITY WEXNER MEDICAL CENTER   (United States)

Author keywords

Apoptosis; BCL 2; cancer; chemotherapy; flow cytometry; mitochondria

Indexed keywords

4 [4 (4' CHLORO 2 BIPHENYLYLMETHYL) 1 PIPERAZINYL] N [4 [3 DIMETHYLAMINO 1 (PHENYLTHIOMETHYL)PROPYLAMINO] 3 NITROBENZENESULFONYL]BENZAMIDE; ALAMETHICIN; BH3 PROTEIN; BIM PROTEIN; BIOLOGICAL MARKER; CYTOCHROME C; EPITHELIAL CELL ADHESION MOLECULE; FORMALDEHYDE; HERMES ANTIGEN; NAVITOCLAX; PROTEIN BAD; PROTEIN BAX; PROTEIN BCL 2;

APOPTOSIS; ARTICLE; CANCER CHEMOTHERAPY; CELL CULTURE; CYTOSOL; DYNAMIC BH3 PROFILING; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN TISSUE; MITOCHONDRIAL MEMBRANE; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; WILD TYPE; CHEMISTRY; METABOLISM; MITOCHONDRION; PROCEDURES; PROTEIN DOMAIN; SECRETION (PROCESS); STAINING; TISSUE FIXATION;

APOPTOSIS; CYTOCHROMES C; FLOW CYTOMETRY; HUMANS; MITOCHONDRIA; PROTEIN DOMAINS; PROTO-ONCOGENE PROTEINS C-BCL-2; STAINING AND LABELING; TISSUE FIXATION;

EID: 84974733613     PISSN: 14316730     EISSN: 14374315     Source Type: Journal    
DOI: 10.1515/hsz-2016-0107     Document Type: Article

References (14)

1. Aragon, J.J., Feliu, J.E., Frenkel, R.A., Sols, A. (1980). Permeabilization of animal cells for kinetic studies of intracellular enzymes: in situ behavior of the glycolytic enzymes of erythrocytes. Proc. Natl. Acad. Sci. USA 77, 6324-6328.
2. Brunelle, J.K., Letai, A. (2009). Control of mitochondrial apoptosis by the Bcl-2 family. J. Cell Sci. 122, 437-441.
3. Cassany, A., Gerace, L. (2009). Reconstitution of nuclear import in permeabilized cells. Methods Mol. Biol. 464, 181-205.
4. Certo, M., Del Gaizo Moore, V., Nishino, M., Wei, G., Korsmeyer, S., Armstrong, S.A., Letai, A. (2006). Mitochondria primed by death signals determine cellular addiction to antiapoptotic BCL-2 family members. Cancer Cell 9, 351-365.
5. Fiskum, G., Craig, S.W., Decker, G.L., Lehninger, A.L. (1980). The cytoskeleton of digitonin-treated rat hepatocytes. Proc. Natl. Acad. Sci. USA 77, 3430-3434.
6. Foight, G.W., Ryan, J.A., Gulla, S.V., Letai, A., Keating, A.E. (2014). Designed BH3 peptides with high affinity and specificity for targeting Mcl-1 in cells. ACS Chem. Biol. 9, 1962-1968.
7. Liu, J., Xiao, N., DeFranco, D.B. (1999). Use of digitonin-permeabilized cells in studies of steroid receptor subnuclear trafficking. Methods 19, 403-409.
8. Mason, K.D., Carpinelli, M.R., Fletcher, J.I., Collinge, J.E., Hilton, A.A., Ellis, S., Kelly, P.N., Ekert, P.G., Metcalf, D., Roberts, A.W., et al. (2007). Programmed anuclear cell death delimits platelet life span. Cell 128, 1173-1186.
9. Montero, J., Sarosiek, K.A., DeAngelo, J.D., Maertens, O., Ryan, J., Ercan, D., Piao, H., Horowitz, N.S., Berkowitz, R.S., Matulonis, U., et al. (2015). Drug-induced death signaling strategy rapidly predicts cancer response to chemotherapy. Cell 160, 977-989.
10. Ni Chonghaile, T., Sarosiek, K.A., Vo, T.T., Ryan, J.A., Tammareddi, A., Moore, V. del G., Deng, J.,erson, K.C., Richardson, P., et al. (2011). Pretreatment mitochondrial priming correlates with clinical response to cytotoxic chemotherapy. Science 334, 1129-1133.
11. Ryan, J., Letai, A. (2013). BH3 profiling in whole cells by fluorimeter or FACS. Methods 61, 156-164.
12. Ryan, J.A., Brunelle, J.K., Letai, A. (2010). Heightened mitochondrial priming is the basis for apoptotic hypersensitivity of CD4CD8thymocytes. Proc. Natl. Acad. Sci. USA 107, 12895-12900.
13. Vo, T.T., Ryan, J., Carrasco, R., Neuberg, D., Rossi, D.J., Stone, R.M., Deangelo, D.J., Frattini, M.G., Letai, A. (2012). Relative mitochondrial priming of myeloblasts and normal HSCs determines chemotherapeutic success in AML. Cell 151, 344-355.
14. Zhang, H., Nimmer, P.M., Tahir, S.K., Chen, J., Fryer, R.M., Hahn, K.R., Iciek, L.A., Morgan, S.J., Nasarre, M.C., Nelson, R., et al. (2007). Bcl-2 family proteins are essential for platelet survival. Cell Death Differ. 14, 943-951.