Reconstitution of nuclear import in permeabilized cells

Methods in Molecular Biology

Volumn 464, Issue , 2008, Pages 181-205

  Cassany, Aurelia ^a   Gerace, Larry ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Digitonin permeabilized cells; Importins; Karyopherins; Nuclear protein import; Ran; Recombinant protein expression; Recombinant protein purification; Shuttling nuclear transport factors

Indexed keywords

EUKARYOTA; MAMMALIA;

EID: 60349118158     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-461-6_11     Document Type: Article

References (19)

1. Fahrenkrog, B., Koser, J., and Aebi U. (2004) The nuclear pore complex: a jack of all trades? Trends Biochem. Sci. 29, 175-182.
2. Mosammaparast, N. and Pemberton, L.F. (2004) Karyopherins: from nuclear-transport mediators to nuclear-function regulators. Trends Cell Biol. 14, 547-556.
3. Macara, I.G. (2001) Transport into and out of the nucleus. Microbiol. Mol. Biol. Rev. 65, 570-594.
4. Pemberton, P.L. and Paschal B. (2005) Mechanisms of receptor-mediated nuclear import and nuclear export. Traffic 6, 187-198.
5. Adam, S.A., Marr, R.S., and Gerace L. (1990) Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors. J. Cell. Biol. 111, 807-816.
6. Chan, R.C. and Forbes, D.J. (2005) In vitro study of nuclear assembly and nuclear import using Xenopus egg extracts. In: Xenopus protocols (Liu, J.X., ed.), Humana Press, Totowa, NJ, pp. 289-300.
7. Colbeau, A., Nachbaur, J., and Vignais, P.M. (1971) Enzymatic characterization and lipid composition of rat liver subcellular membrane. Biochim. Biophys. Acta 249, 462-492.
8. Melchior F. (1998) Nuclear protein import in a permeabilized cell assay, in Protein targeting protocols (Clegg, R.A., ed.), Humana Press, Totowa, NJ, pp. 265-273.
9. Kehlenbach, R.H. and Gerace L. (2002) Analysis of nuclear protein import and export in vitro using fluorescent cargoes, in GTPase protocols (Manser, E. and Leung, T., ed), Humana Press, Totowa, NJ, pp. 231-245.
10. Paraskeva, E., Izaurralde, E., Bischoff, F.R., Huber, J., Kutay, U., Hartmenn, E., Luhrmann, R., and Gorlich, D. (1999) CRM1-mediated recycling of snurportin 1 to the cytoplasm. J. Cell Biol. 145, 255-264.
11. Kalderon, D., Roberts, B. L., Richardson, W. D., and Smith, A.E. (1984) A short amino acid sequence able to specify nuclear location. Cell 39, 499-509.
12. Dingwall, C. and Laskey, R.A. (1991) Nuclear targeting sequences-a consensus? Trends Biochem. Sci. 16, 478-481.
13. Lyman, S.K., Guan, T., Bednenko, J., Wodrich, H., and Gerace, L. (2002) Influence of cargo size on Ran and energy requirements for nuclear protein import. J. Cell Biol. 159, 55-67.
14. Weis, K., Mattaj, I.W., and Lamond, A.I. (1995) Identification of hSRP1 alpha as a functional receptor for nuclear localization sequences. Science 268, 1049-1053.
15. Jakel, S., Albig, W., Kutay, U., Bischoff, F.R., Schwamborn, K., Doenecke, D., and Gorlich, D. (1999) The importin beta/importin 7 heterodimer is a functional nuclear import receptor for histone H1. EMBO J. 18, 2411-2423.
16. Pollard, V.W., Michael, W.M., Nakielny, S., Siomi, M.C., Wang, F., and Dreyfuss G. (1996) A novel receptor-mediated nuclear import pathway. Cell 86, 985-994.
17. Melchior, F., Paschal, B., Evans, J., and Gerace L. (1993) Inhibition of nuclear protein import by nonhydrolyzable analogues of GTP and identification of the small GTPase Ran/TC4 as an essential transport factor. J. Cell Biol. 123, 1649-1659.
18. Paschal, B. and Gerace, L. (1995) Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62. J. Cell Biol. 129, 925-937.
19. Paine, P.L., Moore, L.C., and Horowitz S.B. (1975) Nuclear envelope permeability. Nature 254, 109-114.