A Wide-Field Fluorescence Microscope Extension for Ultrafast Screening of One-Bead One-Compound Libraries Using a Spectral Image Subtraction Approach

ACS Combinatorial Science

Volumn 18, Issue 5, 2016, Pages 209-219

  Heusermann, Wolf ^a   Ludin, Beat ^b   Pham, Nhan T ^c   Auer, Manfred ^c   Weidemann, Thomas ^d   Hintersteiner, Martin ^e  

^a UNIVERSITY OF BASEL   (Switzerland)

^b Life Imaging Services   (Switzerland)

^c UNIVERSITY OF EDINBURGH   (United Kingdom)

^d MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

^e Succursale di Lugano   (Switzerland)

Author keywords

high throughput screening; one bead one compound libraries; spectral imaging; ultrafast screening

Indexed keywords

MICROSPHERE; PEPTIDE LIBRARY; PROTEIN BINDING;

COMBINATORIAL CHEMISTRY; DRUG DEVELOPMENT; FLUORESCENCE MICROSCOPY; HIGH THROUGHPUT SCREENING; PEPTIDE LIBRARY; PROCEDURES; PROTEIN MICROARRAY;

COMBINATORIAL CHEMISTRY TECHNIQUES; DRUG DISCOVERY; HIGH-THROUGHPUT SCREENING ASSAYS; MICROSCOPY, FLUORESCENCE; MICROSPHERES; PEPTIDE LIBRARY; PROTEIN ARRAY ANALYSIS; PROTEIN BINDING;

EID: 84969612636     PISSN: 21568952     EISSN: None     Source Type: Journal    
DOI: 10.1021/acscombsci.5b00175     Document Type: Article

References (29)

1. Kneller, R. The Importance of New Companies for Drug Discovery: Origins of a Decade of New Drugs Nat. Rev. Drug Discovery 2010, 9, 867-882 10.1038/nrd3251
2. Frearson, J.; Wyatt, P. Drug Discovery in Academia- the Third Way? Expert Opin. Drug Discovery 2010, 5, 909-919 10.1517/17460441.2010.506508
3. Ho, Y. et al. Systematic Identification of Protein Complexes in Saccharomyces Cerevisiae by Mass Spectrometry Nature 2002, 415, 180-183 10.1038/415180a
4. Jones, R. B.; Gordus, A.; Krall, J. A.; MacBeath, G. A Quantitative Protein Interaction Network for the ErbB Receptors Using Protein Microarrays Nature 2006, 439, 168-174 10.1038/nature04177
5. Meisner, N. C.; Hintersteiner, M.; Uhl, V.; Weidemann, T.; Schmied, M.; Gstach, H.; Auer, M. The Chemical Hunt for the Identification of Drugable Targets 1 Curr. Opin. Chem. Biol. 2004, 8, 424-431 10.1016/j.cbpa.2004.06.011
6. Kodadek, T. Rethinking Screening Nat. Chem. Biol. 2010, 6, 162-165 10.1038/nchembio.303
7. Diaz-Mochon, J. J.; Bialy, L.; Bradley, M. Dual Colour, Microarray-Based, Analysis of 10 000 Protease Substrates Chem. Commun. 2006, 3984-3986 10.1039/B609029J
8. Kodadek, T. The Rise, Fall and Reinvention of Combinatorial Chemistry Chem. Commun. 2011, 47, 9757 10.1039/c1cc12102b
9. Lam, K. S.; Salmon, S. E.; Hersh, E. M.; Hruby, V. J.; Kazmierski, W. M.; Knapp, R. J. A New Type of Synthetic Peptide Library for Identifying Ligand-Binding Activity Nature 1991, 354, 82-84 10.1038/354082a0
10. Sweeney, M. C.; Wang, X.; Park, J.; Liu, Y.; Pei, D. Determination of the Sequence Specificity of XIAP BIR Domains by Screening a Combinatorial Peptide Library Biochemistry 2006, 45, 14740-14748 10.1021/bi061782x
11. Gocke, A. R.; Udugamasooriya, D. G.; Archer, C. T.; Lee, J.-Y.; Kodadek, T. Isolation of Antagonists of Antigen-Specific Autoimmune T Cell Proliferation Chem. Biol. 2009, 16, 1133-1139 10.1016/j.chembiol.2009.10.011
12. Doran, T. M.; Gao, Y.; Mendes, K.; Dean, S.; Simanski, S.; Kodadek, T. Utility of Redundant Combinatorial Libraries in Distinguishing High and Low Quality Screening Hits ACS Comb. Sci. 2014, 16, 259-270 10.1021/co500030f
13. Auer, M.; Moore, K. J.; Meyer-Almes, F. J.; Guenther, R.; Pope, A. J.; Stoeckli, K. A. Fluorescence Correlation Spectroscopy: Lead Discovery by Miniaturized HTS Drug Discovery Today 1998, 3, 457-465 10.1016/S1359-6446(98)01240-9
14. Hintersteiner, M.; Kimmerlin, T.; Kalthoff, F.; Stoeckli, M.; Garavel, G.; Seifert, J.-M.; Meisner, N.-C.; Uhl, V.; Buehler, C.; Weidemann, T.; Auer, M. Single Bead Labeling Method for Combining Confocal Fluorescence On-Bead Screening and Solution Validation of Tagged One-Bead One-Compound Libraries Chem. Biol. 2009, 16, 724-735 10.1016/j.chembiol.2009.06.011
15. Hintersteiner, M.; Knox, A. J.; Mudd, G.; Auer, M. Towards Mimicking Short Linear Peptide Motifs: Identification of New Mixed A,β-Peptidomimetic Ligands for SLAM-Associated Protein (SAP) by Confocal on-Bead Screening J. Chem. Biol. 2012, 5, 63-79 10.1007/s12154-011-0071-9
16. Townsend, J.; Do, A.; Lehman, A.; Dixon, S.; Sanii, B.; Lam, K. S. 3-Nitro-Tyrosine as an Internal Quencher of Autofluorescence Enhances the Compatibility of Fluorescence Based Screening of OBOC Combinatorial Libraries Comb. Chem. High Throughput Screening 2010, 13, 422-429 10.2174/138620710791292994
17. Olivos, H. J.; Bachhawat-Sikder, K.; Kodadek, T. Quantum Dots as a Visual Aid for Screening Bead-Bound Combinatorial Libraries ChemBioChem 2003, 4, 1242-1245 10.1002/cbic.200300712
18. Astle, J. M.; Simpson, L. S.; Huang, Y.; Reddy, M. M.; Wilson, R.; Connell, S.; Wilson, J.; Kodadek, T. Seamless Bead to Microarray Screening: Rapid Identification of the Highest Affinity Protein Ligands from Large Combinatorial Libraries Chem. Biol. 2010, 17, 38-45 10.1016/j.chembiol.2009.12.015
19. Liu, T.; Qian, Z.; Xiao, Q.; Pei, D. High-Throughput Screening of One-Bead-One-Compound Libraries: Identification of Cyclic Peptidyl Inhibitors against Calcineurin/NFAT Interaction ACS Comb. Sci. 2011, 13, 537-546 10.1021/co200101w
20. Mendes, K.; Ndungu, J. M.; Clark, L. F.; Kodadek, T. Optimization of the Magnetic Recovery of Hits from One-Bead-One-Compound Library Screens ACS Comb. Sci. 2015, 17, 506-517 10.1021/acscombsci.5b00090
21. Muller, K.; Gombert, F. O.; Manning, U.; Grossmueller, F.; Graff, P.; Zaegel, H.; Zuber, J. F.; Freuler, F.; Tschopp, C.; Baumann, G. Rapid Identification of Phosphopeptide Ligands for SH2 Domains. Screening of Peptide Libraries by Fluorescence-Activated Bead Sorting J. Biol. Chem. 1996, 271, 16500-16505 10.1074/jbc.271.28.16500
22. Pulak, R. Techniques for Analysis, Sorting, and Dispensing of C. Elegans on the COPAS Flow-Sorting System Methods Mol. Biol. 2006, 351, 275-286 10.1385/1-59745-151-7:275
23. Reddy, M. M.; Bachhawat-Sikder, K.; Kodadek, T. Transformation of Low-Affinity Lead Compounds into High-Affinity Protein Capture Agents Chem. Biol. 2004, 11, 1127-1137 10.1016/j.chembiol.2004.05.013
24. Hintersteiner, M.; Auer, M. A Two-Channel Detection Method for Autofluorescence Correction and Efficient on-Bead Screening of One-Bead One-Compound Combinatorial Libraries Using the COPAS Fluorescence Activated Bead Sorting System Methods Appl. Fluoresc. 2013, 1, 017001 10.1088/2050-6120/1/1/017001
25. Hintersteiner, M.; Buehler, C.; Uhl, V.; Schmied, M.; Muller, J.; Kottig, K.; Auer, M. Confocal Nanoscanning, Bead Picking (CONA): PickoScreen Microscopes for Automated and Quantitative Screening of One-Bead One-Compound Libraries J. Comb. Chem. 2009, 11, 886-894 10.1021/cc900059q
26. Hintersteiner, M.; Buehler, C.; Auer, M. On-Bead Screens Sample Narrower Affinity Ranges of Protein-Ligand Interactions Compared to Equivalent Solution Assays ChemPhysChem 2012, 13, 3472-3480 10.1002/cphc.201200117
27. Hong, H.; Lee, H. Y.; Kwak, W.; Yoo, J.; Na, M.-H.; So, I. S.; Kwon, T.-H.; Park, H.-S.; Huh, S.; Oh, G. T.; Kwon, I.-C.; Kim, I.-S.; Lee, B.-H. Phage Display Selection of Peptides That Home to Atherosclerotic Plaques: IL-4 Receptor as a Candidate Target in Atherosclerosis J. Cell. Mol. Med. 2008, 12, 2003-2014 10.1111/j.1582-4934.2008.00189.x
28. Lee, J. H.; Engler, J. A.; Collawn, J. F.; Moore, B. A. Receptor Mediated Uptake of Peptides That Bind the Human Transferrin Receptor Eur. J. Biochem. 2001, 268, 2004-2012 10.1046/j.1432-1327.2001.02073.x
29. Hintersteiner, M.; Kimmerlin, T.; Garavel, G.; Schindler, T.; Bauer, R.; Meisner, N. C.; Seifert, J.-M.; Uhl, V.; Auer, M. A Highly Potent and Cellularly Active B-Peptidic Inhibitor of the p53/hDM2 Interaction ChemBioChem 2009, 10, 994-998 10.1002/cbic.200800803