Mining of Ruminant Microbial Phytase (RPHY1) from Metagenomic Data of Mehsani Buffalo Breed: Identification, Gene Cloning, and Characterization

Journal of Molecular Microbiology and Biotechnology

Volumn 26, Issue 4, 2016, Pages 252-260

  Mootapally, Chandra Shekar ^a   Nathani, Neelam M ^a,b   Patel, Amrutlal K ^c   Jakhesara, Subhash J ^a   Joshi, Chaitanya G ^a  

^a Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya   (India)

^b SAURASHTRA UNIVERSITY   (India)

^c Hester Pharmceuticals Limited   (India)

Author keywords

Characterization; Cloning; Metagenomics; Phytase; Prevotella ruminicola; Rumen

Indexed keywords

HYBRID PROTEIN; PHYTASE; POLYHISTIDINE TAG; RUMINANT MICROBIAL PHYTASE 1; THIOREDOXIN; UNCLASSIFIED DRUG; ENZYME INHIBITOR; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BUFFALO; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; EXPRESSION VECTOR; GENE IDENTIFICATION; GENOME ANALYSIS; ISOELECTRIC POINT; LIGAND BINDING; METAGENOMICS; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PREVOTELLA; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; RUMINANT MICROBIAL PHYTASE 1 GENE; AFFINITY CHROMATOGRAPHY; ANIMAL; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METAGENOME; MICROBIOLOGY; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; RUMEN; SEQUENCE HOMOLOGY; TEMPERATURE;

6-PHYTASE; ANIMALS; BUFFALOES; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME INHIBITORS; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; METAGENOME; MOLECULAR WEIGHT; PREVOTELLA; RECOMBINANT PROTEINS; RUMEN; SEQUENCE HOMOLOGY; TEMPERATURE;

EID: 84967000333     PISSN: 14641801     EISSN: 16602412     Source Type: Journal    
DOI: 10.1159/000445321     Document Type: Article

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