High level expression of an acid-stable phytase from citrobacter freundii in pichia pastoris

Applied Biochemistry and Biotechnology

Volumn 162, Issue 8, 2010, Pages 2157-2165

  Zhao, Wei ^a   Xiong, Aisheng ^a   Fu, Xiaoyan ^a   Gao, Feng ^a   Tian, Yongsheng ^a   Peng, Rihe ^a  

^a INSTITUTE OF EDIBLE FUNGI   (China)

Author keywords

Citrobacter freundii; Codon optimization; pH stable; Phytase; Pichia pastoris

Indexed keywords

ACTIVITY ASSAYS; AFFINITY COLUMN; AGAROSE; CITROBACTER FREUNDII; CODON OPTIMIZATION; HIGH LEVEL EXPRESSION; OPTIMAL TEMPERATURE; PH OPTIMA; PHYTASE GENE; PHYTASES; PICHIA PASTORIS; POTENTIAL APPLICATIONS; RECOMBINANT PHYTASE; SODIUM PHYTATE; SPECIFIC ACTIVITY;

ACIDS; GENE EXPRESSION; OPTIMIZATION; SODIUM;

ENZYME ACTIVITY;

PHYTASE;

ARTICLE; CITROBACTER FREUNDII; INCUBATION TEMPERATURE; NONHUMAN; PH; PICHIA PASTORIS; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

6-PHYTASE; CITROBACTER FREUNDII; CLONING, MOLECULAR; CODON; ENZYME STABILITY; FOOD ADDITIVES; GENE EXPRESSION; GENETIC VECTORS; GLYCOSYLATION; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; PICHIA; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; TEMPERATURE;

CITROBACTER FREUNDII; PICHIA PASTORIS;

EID: 78149471361     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12010-010-8990-4     Document Type: Article

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