Expression of a bacillus phytase C gene in pichia pastoris and properties of the recombinant enzyme

Applied and Environmental Microbiology

Volumn 76, Issue 16, 2010, Pages 5601-5608

  Guerrero Olazarán, Martha ^a   Rodríguez Blanco, Lilí ^a   Carreon Treviño, J Gerardo ^a   Gallegos López, Juan A ^a   Viader Salvadó, José M ^a  

^a UNIVERSIDAD AUTÓNOMA DE NUEVO LEÓN   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOLOGY; GENE ENCODING; GENE EXPRESSION; SHELLFISH; YEAST;

BIOCHEMICAL PROPERTIES; CALCIUM CONCENTRATION; CLONING AND EXPRESSION; RECOMBINANT BACILLI; RECOMBINANT ENZYMES; RECOMBINANT PHYTASE; RECOMBINANT STRAINS; RESIDUAL ACTIVITY;

ENZYMES;

BIOCHEMISTRY; CALCIUM; CLONAL GROWTH; CRUSTACEAN; ENZYME ACTIVITY; GENE EXPRESSION; METHANOL; PH; THERMOREGULATION; YEAST;

BACILLUS SUBTILIS; DECAPODA (CRUSTACEA); PICHIA PASTORIS; SUS;

BACTERIAL PROTEIN; CALCIUM CHLORIDE; COENZYME; PHYTASE; RECOMBINANT PROTEIN; TRYPSIN;

BACILLUS SUBTILIS; CHEMISTRY; COENZYME; ENZYME STABILITY; ENZYMOLOGY; GENE EXPRESSION; GENE VECTOR; GENETICS; GLYCOSYLATION; METABOLISM; MOLECULAR CLONING; MOLECULAR WEIGHT; PH; PICHIA; PROTEIN STABILITY; TEMPERATURE;

6-PHYTASE; BACILLUS SUBTILIS; BACTERIAL PROTEINS; CALCIUM CHLORIDE; CLONING, MOLECULAR; COENZYMES; ENZYME STABILITY; GENE EXPRESSION; GENETIC VECTORS; GLYCOSYLATION; HYDROGEN-ION CONCENTRATION; MOLECULAR WEIGHT; PICHIA; PROTEIN STABILITY; RECOMBINANT PROTEINS; TEMPERATURE; TRYPSIN;

EID: 77955958887     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00762-10     Document Type: Article

References (40)

1. Cao, L., W. Wang, C. Yang, Y. Yang, J. Diana, A. Yakupitiyage, Z. Luo, andD. Li. 2007. Application of microbial phytase in fish feed. Enzyme Microb. Technol. 40: 497-507.
2. Choi, Y. M., H. J. Suh, andJ. M. Kim. 2001. Purification and properties of extracellular phytase from Bacillus sp. KHU-10. J. Protein Chem. 20: 287-292.
3. Clark, S. E., E. H. Muslin, andC. H. Henson. 2004. Effect of adding and removing N-glycosylation recognition sites on the thermostability of barley α-glucosidase. Protein Eng. Des. Sel. 17: 245-249.
4. Daly, R., andM. T. Hearn. 2005. Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production. J. Mol. Recognit. 18: 119-138.
5. Ecamilla-Treviño, L. L., J. M. Viader-Salvadó, H. A. Barrera-Saldaña, andM. Guerrero-Olazarán. 2000. Biosynthesis and secretion of recombinant human growth hormone in Pichia pastoris. Biotechnol. Lett. 22: 109-114.
6. Farhat, A., H. Chouayekh, M. B. Farhat, K. Bouchaala, andS. Bejar. 2008. Gene cloning and characterization of a thermostable phytase from Bacillus subtilis US417 and assessment of its potential as a feed additive in comparison with a commercial enzyme. Mol. Biotechnol. 40: 127-135.
7. Fiske, C. H., andY. Subarrow. 1925. The colorimetric determination of phosphorus. J. Biol. Chem. 66: 375-400.
8. Fu, S., J. Sun, L. Qian, andZ. Li. 2008. Bacillus phytases: present scenario and future perspectives. Appl. Biochem. Biotechnol. 151: 1-8.
9. Greiner, R., andU. Konietzny. 2006. Phytase for food application. Food Technol. Biotechnol. 44: 125-140.
10. Guo, M., H. Hang, T. Zhu, Y. Zhuang, J. Chu, andS. Zhang. 2008. Effect of glycosylation on biochemical characterization of recombinant phytase expressed in Pichia pastoris. Enzyme Microb. Technol. 42: 340-345.
11. Ha, N. C., B. C. Oh, S. Shin, H. J. Kim, T. K. Oh, Y. O. Kim, K. Y. Choi, andB. H. Oh. 2000. Crystal structures of a novel thermostable phytase in partially and fully calcium-loaded states. Nat. Struct. Biol. 7: 147-153.
12. Haefner, S., A. Knietsch, E. Scholten, J. Braun, M. Lohscheidt, andO. Zelder. 2005. Biotechnological production and applications of phytases. Appl. Microbiol. Biotechnol. 68: 588-597.
13. Han, Y. M., andX. G. Lei. 1999. Role of glycosylation in the functional expression of an Aspergillus niger phytase (phyA) in Pichia pastoris. Arch. Biochem. Biophys. 364: 83-90.
14. Ilgen, C., J. Lin-Cereghino, andJ. M. Cregg. 2004. Pichia pastoris, p. 143-162. In G. Gellissen (ed.), Production of recombinant proteins-novel microbial and eukaryotic expression systems. Wiley-VCH, Weinheim, Germany.
15. Kerovuo, J., I. Lappalainen, andT. Reinikainen. 2000. The metal dependence of Bacillus subtilis phytase. Biochem. Biophys. Res. Commun. 268: 365-369.
16. Kerovuo, J., J. Rouvinen, andF. Hatzack. 2000. Analysis of myo-inositol hexakisphosphate hydrolysis by Bacillus phytase: indication of a novel reaction mechanism. Biochem. J. 352: 623-628.
17. Kerovuo, J., M. Lauraeus, P. Nurminen, N. Kalkkinen, andJ. Apajalahti. 1998. Isolation, characterization, molecular gene cloning, and sequencing of a novel phytase from Bacillus subtilis. Appl. Environ. Microbiol. 64: 2079-2085.
18. Kerovuo, J., andS. Tynkkynen. 2000. Expression of Bacillus subtilis phytase in Lactobacillus plantarum 755. Lett. Appl. Microbiol. 30: 325-329.
19. Kim, D. H., B. C. Oh, W. C. Choi, J. K. Lee, andT. K. Oh. 1999. Enzymatic evaluation of Bacillus amyloliquefaciens phytase as a feed additive. Biotechnol. Lett. 20: 925-927.
20. Kim, Y. O., H. K. Kim, K. S. Bae, J. H. Yu, andT. K. Oh. 1998. Purification and properties of a thermostable phytase from Bacillus sp. DS11. Enzyme Microb. Technol. 22: 2-7.
21. Kim, Y. O., J. K. Lee, B. C. Oh, andT. K. Oh. 1999. High-level expression of a recombinant thermostable phytase in Bacillus subtilis. Biosci. Biotechnol. Biochem. 63: 2205-2207.
22. Lei, X. G., J. M. Porres, E. J. Mullaney, andH. Brinch-Pedersen. 2007. Phytase: source, structure and application, p. 505-529. In J. Polaina and A. P. MacCabe (ed.), Industrial enzymes: structure, function and applications. Springer, Dordrecht, Netherlands.
23. Liebert, F., andL. Portz. 2005. Nutrient utilization of Nile tilapia Oreochromis niloticus fed plant based low phosphorus diets supplemented with graded levels of different sources of microbial phytase. Aquaculture 248: 111-119.
24. Macauley-Patrick, S., M. L. Fazenda, B. McNeil, andL. M. Harvey. 2005. Heterologous protein production using the Pichia pastoris expression system. Yeast 22: 249-270.
25. Montesino, R., R. García, O. Quintero, andJ. A. Cremata. 1998. Variation in N-linked oligosaccharide structures on heterologous proteins secreted by the methylotrophic yeast Pichia pastoris. Protein Expr. Purif. 14: 197-207.
26. Mullaney, E. J., andA. H. Ullah. 2007. Phytases: attributes, catalytic mechanisms and applications, p. 97-110. In L. Turner, A. E. Richardson, and E. J. Mullaney (ed.), Inositol phosphates: linking agriculture and the environment. CAB International, Oxfordshire, United Kingdom.
27. Oh, B. C., W. C. Choi, S. Park, Y. O. Kim, andT. K. Oh. 2004. Biochemical properties and substrate specificities of alkaline and histidine acid phytases. Appl. Microbiol. Biotechnol. 63: 362-372.
28. Powar, V. K., andV. Jagannathan. 1982. Purification and properties of phytase-specific phosphatase from Bacillus subtilis. J. Bacteriol. 151: 102-108.
29. Rao, D. E., K. V. Rao, and V. D. Reddy. 2008. Cloning and expression of Bacillus phytase gene (phy) in Escherichia coli and recovery of active enzyme from the inclusion bodies. J. Appl. Microbiol. 105: 1128-1137.
30. Rodriguez, E., E. J. Mullaney, andX. G. Lei. 2000. Expression of the Aspergillus fumigatus phytase gene in Pichia pastoris and characterization of the recombinant enzyme. Biochem. Biophys. Res. Commun. 268: 373-378.
31. Rodriguez, E., J. M. Porres, Y. Han, andX. G. Lei. 1999. Different sensitivity of recombinant Aspergillus niger phytase (r-PhyA) and Escherichia coli pH 2.5 acid phosphatase (r-AppA) to trypsin and pepsin in vitro. Arch. Biochem. Biophys. 365: 262-267.
32. Rodriguez, E., Z. A. Wood, P. A. Karplus, andX. G. Lei. 2000. Site-directed mutagenesis improves catalytic efficiency and thermostability of Escherichia coli pH 2.5 acid phosphatase/phytase expressed in Pichia pastoris. Arch. Biochem. Biophys. 382: 105-112.
33. Sambrook, J., andD. W. Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
34. Shin, S., N. C. Ha, B. C. Oh, T. K. Oh, andB. H. Oh. 2001. Enzyme mechanism and catalytic property of beta propeller phytase. Structure 9: 851-858.
35. Spiro, R. G. 2002. Protein glycosylation: nature, distribution, enzymatic formation, and disease implications of glycopeptide bonds. Glycobiology 12: 43R-56R.
36. Tye, A. J., F. K. Siu, T. Y. Leung, andB. L. Lim. 2002. Molecular cloning and the biochemical characterization of two novel phytases from B. subtilis 168 and B. licheniformis. Appl. Microbiol. Biotechnol. 59: 190-197.
37. Vuolanto, A., N. V. Weymarn, J. Kerovuo, H. Ojamo, andM. Leisola. 2001. Phytase production by high cell density culture of recombinant Bacillus subtilis. Biotechnol. Lett. 23: 761-766.
38. Wang, H. N., Q. Wu, H. X. Zhao, H. Chen, andP. Liu. 2005. Secretory expression of Bacillus subtilis phytase phyC in Pichia pastoris. J. Zhejiang Univ. (Agric. Life Sci.) 31: 621-627.
39. Wyss, M., L. Pasamontes, R. Rémy, J. Kohler, E. Kusznir, M. Gadient, F. Müller, andA. P. G. M. van Loon. 1998. Comparison of the thermostability properties of three acid phosphatases from molds: Aspergillus fumigatus phytase, A. niger phytase, and A. niger pH 2.5 acid phosphatase. Appl. Environ. Microbiol. 64: 4446-4451.
40. Zou, L. K., H. N. Wang, X. Pan, T. Xie, Q. Wu, Z. W. Xie, andW. R. Zhou. 2006. Design and expression of synthetic phyC gene encoding the neutral phytase in Pichia pastoris. Acta Biochim. Biophys. Sin. (Shanghai) 38: 803-811.