Development of monoclonal antibody and diagnostic test for Middle East respiratory syndrome coronavirus using cell-free synthesized nucleocapsid antigen

Frontiers in Microbiology

Volumn 7, Issue APR, 2016, Pages

  Yamaoka, Yutaro ^a,b   Matsuyama, Shutoku ^c   Fukushi, Shuetsu ^c   Matsunaga, Satoko ^a   Matsushima, Yuki ^a,d   Kuroyama, Hiroyuki ^b   Kimura, Hirokazu ^c   Takeda, Makoto ^c   Chimuro, Tomoyuki ^b   Ryo, Akihide ^a  

^a YOKOHAMA CITY UNIVERSITY   (Japan)

^b KANTO CHEMICAL CO INC   (Japan)

^c NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

^d KAWASAKI CITY INSTITUTE FOR PUBLIC HEALTH   (Japan)

Author keywords

Antigen; Cell free protein synthesis; Detection; Diagnosis; MERS coronavirus; Monoclonal antibody; Nucleocapsid

Indexed keywords

MONOCLONAL ANTIBODY; NUCLEOCAPSID PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY PRODUCTION; ARTICLE; BIOINFORMATICS; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; HYBRIDOMA CELL CULTURE; IMMUNIZATION; IMMUNOAFFINITY CHROMATOGRAPHY; IMMUNOBLOTTING; IMMUNOPRECIPITATION; MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS; MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS INFECTION; MOUSE; NONHUMAN; POINT OF CARE TESTING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SARS CORONAVIRUS; STRUCTURAL HOMOLOGY; VIRUS INFECTION;

EID: 84966393702     PISSN: None     EISSN: 1664302X     Source Type: Journal    
DOI: 10.3389/fmicb.2016.00509     Document Type: Article

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