Wheat germ cell-free system-based production of hemagglutinin-neuraminidase glycoprotein of human parainfluenza virus type 3 for generation and characterization of monoclonal antibody

Frontiers in Microbiology

Volumn 5, Issue MAY, 2014, Pages

  Matsunaga, Satoko ^a   Kawakami, Shiho ^a   Matsuo, Izumi ^a   Okayama, Akiko ^a   Tsukagoshi, Hiroyuki ^b   Kudoh, Ayumi ^a   Matsushima, Yuki ^c   Shimizu, Hideaki ^c   Okabe, Nobuhiko ^c   Hirano, Hisashi ^a   Yamamoto, Naoki ^d   Kimura, Hirokazu ^e   Ryo, Akihide ^a  

^a YOKOHAMA CITY UNIVERSITY   (Japan)

^b GUNMA PREFECTURAL INSTITUTE OF PUBLIC HEALTH AND ENVIRONMENTAL SCIENCES   (Japan)

^c Kawasaki City Health and Safety Research Center   (Japan)

^d NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^e NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

Author keywords

Cell free protein synthesis; Human parainfluenza virus 3; Monoclonal antibody; Proteomics

Indexed keywords

HN PROTEIN; MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ANTIBODY SPECIFICITY; ARTICLE; CELL FREE SYSTEM; CELL FUSION; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; EXPRESSION VECTOR; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HYBRIDOMA; IMMUNIZATION; IMMUNOBLOTTING; IMMUNOFLUORESCENCE TEST; IMMUNOPRECIPITATION; LIQUID CHROMATOGRAPHY; MASSS PECTROMETRY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PARAINFLUENZA VIRUS 3; PARAINFLUENZA VIRUS INFECTION; POLYMERASE CHAIN REACTION; PROTEIN BINDING; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; PROTEOMICS; WHEAT GERM;

EID: 84905060629     PISSN: None     EISSN: 1664302X     Source Type: Journal    
DOI: 10.3389/fmicb.2014.00208     Document Type: Article

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