Estimates of soil bacterial ribosome content and diversity are significantly affected by the nucleic acid extraction method employed

Applied and Environmental Microbiology

Volumn 82, Issue 9, 2016, Pages 2595-2607

  Wüst, Pia K ^a   Nacke, Heiko ^b   Kaiser, Kristin ^b   Marhan, Sven ^c   Sikorski, Johannes ^a   Kandeler, Ellen ^c   Daniel, Rolf ^b   Overmann, Jörg ^a  

^a LEIBNIZ INSTITUTE DSMZ GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES   (Germany)

^b UNIVERSITY OF GÖTTINGEN   (Germany)

^c UNIVERSITY OF HOHENHEIM   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOMOLECULES; EXTRACTION; GENE ENCODING; GENES; MACROMOLECULES; NUCLEIC ACIDS; RNA; SOILS;

454-PYROSEQUENCING; BACTERIAL RIBOSOMES; EXTRACTION PROTOCOLS; HIGH RESOLUTION ANALYSIS; NUCLEIC ACID EXTRACTS; ORDERS OF MAGNITUDE; POTENTIAL ACTIVITIES; RELATIVE ABUNDANCE;

POLYMERASE CHAIN REACTION;

ABUNDANCE ESTIMATION; BACTERIUM; CELL ORGANELLE; EXTRACTION METHOD; GENETIC ANALYSIS; NUCLEIC ACID; SOIL MICROORGANISM; SOIL PROPERTY; SOIL TYPE; SPECIES DIVERSITY;

ACTINOBACTERIA; BACTERIA (MICROORGANISMS); PROTEOBACTERIA;

BACTERIAL DNA; BACTERIAL RNA; NUCLEIC ACID; RNA 16S; SOIL;

BACTERIUM; BIODIVERSITY; CHEMISTRY; DNA SEQUENCE; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; PHYLOGENY; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; RIBOSOME; SOIL;

BACTERIA; BACTERIOLOGICAL TECHNIQUES; BIODIVERSITY; DNA, BACTERIAL; NUCLEIC ACIDS; PHYLOGENY; REAL-TIME POLYMERASE CHAIN REACTION; RIBOSOMES; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SOIL; SOIL MICROBIOLOGY;

EID: 84965173148     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00019-16     Document Type: Article

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