An improved method to extract RNA from soil with efficient removal of humic acids

Journal of Applied Microbiology

Volumn 107, Issue 4, 2009, Pages 1168-1177

  Wang, Y ^a   Morimoto, S ^a   Ogawa, N ^b   Oomori, T ^c   Fujii, T ^a  

^a NATIONAL INSTITUTE FOR AGRO ENVIRONMENTAL SCIENCES   (Japan)

^b SHIZUOKA UNIVERSITY   (Japan)

^c Agricultural Experiment Station of Ehime Prefecture   (Japan)

Author keywords

Bacteria; Humic acids; Real time polymerase chain reaction; RNA extraction; Soil

Indexed keywords

BIODEGRADATION; CHAINS; EXTRACTION; ORGANIC ACIDS; POLYMERASE CHAIN REACTION; RNA; SOILS; TRANSCRIPTION;

BACTERIAL GENES; CHLOROAROMATICS; EXTRACTION METHOD; GENES EXPRESSION; HUMIC ACID; HUMIC SUBSTANCES; REAL TIME POLYMERASE CHAIN REACTIONS; REAL- TIME; REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION; RNA EXTRACTION;

BACTERIA;

3 CHLOROBENZOIC ACID; FULVIC ACID; HUMIC ACID; RNA;

BACTERIUM; EXTRACTION METHOD; FLUORESCENCE SPECTROSCOPY; FULVIC ACID; GENE EXPRESSION; HUMIC ACID; INOCULATION; LYSIS; OPTIMIZATION; POLYMERASE CHAIN REACTION; REAL TIME; RNA; SOIL AMENDMENT; SOIL MICROORGANISM;

ARTICLE; BACTERIAL GENE; BACTERIUM DETECTION; CONTROLLED STUDY; DNA TEMPLATE; ENZYME MECHANISM; GENE EXPRESSION; HUMIC SUBSTANCE; INOCULATION; LYSIS; NONHUMAN; PLASMID; PSEUDOMONAS PUTIDA; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SOIL; SOIL MICROFLORA; SPECTROFLUOROMETRY;

BACTERIA (MICROORGANISMS); PSEUDOMONAS PUTIDA;

EID: 70349137950     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2009.04298.x     Document Type: Article

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