Assessment of commercial chromogenic solid media for the detection of non-O157 Shiga toxin-producing Escherichia coli (STEC)

Diagnostic Microbiology and Infectious Disease

Volumn 85, Issue 3, 2016, Pages 302-308

  Zelyas, Nathan ^a   Poon, Alan ^b   Patterson Fortin, Laura ^b   Johnson, Roger P ^c   Lee, Winki ^b   Chui, Linda ^a,b  

^a UNIVERSITY OF ALBERTA   (Canada)

^b PROVINCIAL LABORATORY FOR PUBLIC HEALTH   (Canada)

^c PUBLIC HEALTH AGENCY OF CANADA   (Canada)

Author keywords

CHROMagar STEC; Real time PCR; Shiga toxin producing Escherichia coli

Indexed keywords

AGAR MEDIUM; ARTICLE; BACTERIAL GROWTH; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CHROMOGENIC MEDIA; CONTROLLED STUDY; CORRELATION ANALYSIS; DIAGNOSTIC TEST ACCURACY STUDY; INTERMETHOD COMPARISON; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PREDICTIVE VALUE; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; CHEMISTRY; COMPARATIVE STUDY; CULTURE MEDIUM; ESCHERICHIA COLI INFECTIONS; EVALUATION STUDY; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MICROBIAL SENSITIVITY TEST; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; PROCEDURES;

ANTIINFECTIVE AGENT; CHROMOGENIC SUBSTRATE; CULTURE MEDIUM; TELLURIUM; TELLUROUS ACID;

ANTI-BACTERIAL AGENTS; BACTERIOLOGICAL TECHNIQUES; CHROMOGENIC COMPOUNDS; CULTURE MEDIA; ESCHERICHIA COLI INFECTIONS; HUMANS; MICROBIAL SENSITIVITY TESTS; PREDICTIVE VALUE OF TESTS; SENSITIVITY AND SPECIFICITY; SHIGA-TOXIGENIC ESCHERICHIA COLI; TELLURIUM;

EID: 84964894911     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2016.03.013     Document Type: Article

References (39)

1. Becton, Dickinson and Company (BD), BBL™ CHROMagar® O157 product insert. 2008, BD, Franklin Lakes, NJ, USA.
2. Bettelheim, K.A., Reliability of CHROMagar® O157 for the detection of enterohemorrhagic Escherichia coli (EHEC) O157 but not EHEC belonging to other serogroups. J Appl Microbiol 85 (1998), 425–428.
3. Bettelheim, K.A., Studies of Escherichia coli cultured on Rainbow™ agar O157 with particular reference to enterohemorrhagic Escherichia coli (EHEC). Microbiol Immunol 42 (1998), 265–269.
4. Brooks, J.T., Bergmire-Sweat, D., Kennedy, M., Hendricks, K., Garcia, M., Marengo, L., et al. Outbreak of shiga toxin–producing Escherichia coli O111:H8 infections among attendees of a high school cheerleading camp. Clin Infect Dis 38 (2004), 190–198.
5. Buchholz, U., Bernard, H., Werber, D., Böhmer, M.M., Remschmidt, C., Wilking, H., et al., an der Heiden, M., Adlhoch, C., Dreesman, J., Ehlers, J., Ethelberg, S., Faber, M., Frank, C., Fricke, G., Greiner, M., Höhle, M., Ivarsson, S., Jark, U., Kirchner, M., Koch, J., Krause, G., Luber, P., Rosner, B., Stark, K., Kühne, M., German outbreak of Escherichia coli O104:H4 associated with sprouts. N Engl J Med 365 (2011), 1763–1770.
6. Chui, L., Couturier, M.R., Chiu, T., Wang, G., Olson, A.B., McDonald, R.R., et al. Comparison of Shiga toxin-producing Escherichia coli detection methods using clinical stool samples. J Mol Diagn 12 (2010), 469–475.
7. Chui, L., Lee, M.-C., Allen, R., Byrks, A., Haines, L., Boras, V., Comparison between ImmunoCard STAT!® and real-time PCR as screening tools for both O157:H7 and non-O157 shiga toxin-producing Escherichia coli (STEC) in southern Alberta, Canada. Diagn Microbiol Infect Dis 77 (2013), 8–13.
8. Chui, L., Lee, M.-C., Malejczyk, K., Lim, L., Fok, D., Kwong, P., Prevalence of shiga toxin-producing Escherichia coli as detected by enzyme-linked immunoassays and real-time PCR during the summer months in northern Alberta, Canada. J Clin Microbiol 49 (2011), 4307–4310.
9. Chui, L., Patterson-Fortin, L., Kuo, J., Li, V., Boras, V., Evaluation of enzyme immunoassays and real-time PCR for detecting Shiga toxin-producing Escherichia coli in Southern Alberta, Canada. J Clin Microbiol 53 (2015), 1019–1023.
10. Church, D.L., Emshey, D., Semeniuk, H., Lloyd, T., Pitout, J.D., Evaluation of BBL CHROMagar O157 versus sorbitol-MacConkey medium for routine detection of Escherichia coli O157 in a centralized regional clinical microbiology laboratory. J Clin Microbiol 45 (2007), 3098–3100.
11. Couturier, M.R., Lee, B., Zelyas, N., Chui, L., Shiga toxigenic Escherichia coli (STEC) detection in stool samples screened for viral gastroenteritis in Alberta, Canada. J Clin Microbiol 49 (2011), 574–578.
12. Feng, P.C., Jinneman, K., Scheutz, F., Monday, S.R., Specificity of PCR and serological assays in the detection of Escherichia coli Shiga toxin subtypes. Appl Environ Microbiol 77 (2011), 6699–6702.
13. Gannon, V.P., King, R.K., Kim, J.Y., Thomas, E.J., Rapid and sensitive method for detection of shiga-like toxin-producing Escherichia coli in ground beef using the polymerase chain reaction. Appl Environ Microbiol 58 (1992), 3809–3815.
14. Gill, A., Huszczynski, G., Gauthier, M., Blais, B., Evaluation of eight agar media for the isolation of shiga toxin-producing Escherichia coli. J Microbiol Methods 96 (2014), 6–11.
15. Gilmour, M.W., Chui, L., Chiu, T., Tracz, D.M., Hagedorn, K., Tschetter, L., et al. Isolation and detection of Shiga toxin-producing Escherichia coli in clinical stool samples using conventional and molecular methods. J Med Microbiol 58 (2009), 905–911.
16. Gouali, M., Ruckly, C., Carle, I., Lejay-Collin, M., Weill, F.X., Evaluation of CHROMagar STEC and STEC O104 chromogenic agar media for detection of shiga toxin-producing Escherichia coli in stool specimens. J Clin Microbiol 51 (2013), 894–900.
17. Gould, L.H., Bopp, C., Strockbine, N., Atkinson, R., Baselski, V., Body, B., et al. Gerner-Smidt P; Centers for Disease Control and Prevention (CDC). Recommendations for diagnosis of shiga toxin-producing Escherichia coli infections by clinical laboratories. MMWR Recomm Rep 58 (2009), 1–14.
18. Hirvonen, J.J., Siitonen, A., Kaukoranta, S.S., Usability and performance of CHROMagar STEC medium in detection of shiga toxin-producing Escherichia coli strains. J Clin Microbiol 50 (2012), 3586–3590.
19. Holland, J.L., Louie, L., Simor, A.E., Louie, M., PCR detection of Escherichia coli O157:H7 directly from stools: evaluation of commercial extraction methods for purifying fecal DNA. J Clin Microbiol 38 (2000), 4108–4113.
20. Johnson, K.E., Thorpe, C.M., Sears, C.L., The emerging clinical importance of non-O157 shiga toxin-producing Escherichia coli. Clin Infect Dis 43 (2006), 1587–1595.
21. Karmali, M.A., Infection by verocytotoxin-producing Escherichia coli. Clin Microbiol Rev 2 (1989), 15–38.
22. Karmali, M.A., Steele, B.T., Petric, M., Lim, C., Sporadic cases of haemolytic-uraemic syndrome associated with faecal cytotoxin and cytotoxin-producing Escherichia coli in stools. Lancet 321 (1983), 619–620.
23. Konowalchuk, J., Speirs, J.I., Stavric, S., Vero response to a cytotoxin of Escherichia coli. Infect Immun 18 (1977), 775–779.
24. 2e in drinking water contaminated by waste water in Finland. Curr Microbiol 62 (2011), 1239–1244.
25. Lindstedt, B.A., Brandal, L.T., Aas, L., Vardund, T., Kapperud, G., Study of polymorphic variable-number of tandem repeats loci in the ECOR collection and in a set of pathogenic Escherichia coli and Shigella isolates for use in a genotyping assay. J Microbiol Methods 69 (2007), 197–205.
26. March, S.B., Ratnam, S., Sorbitol-MacConkey medium for detection of Escherichia coli O157:H7 associated with hemorrhagic colitis. J Clin Microbiol 23 (1986), 869–872.
27. McCallum, C., McGregor, A., Vanniasinkam, T., Prevalence of Shiga toxin-producing Escherichia coli (STEC) in Tasmania, Australia. Pathology 45 (2013), 681–688.
28. McCarthy, T.A., Barrett, N.L., Hadler, J.L., Salsbury, B., Howard, R.T., Dingman, D.W., et al. Hemolytic-uremic syndrome and Escherichia coli O121 at a lake in Connecticut, 1999. Pediatrics, 108, 2001, E59.
29. Milley, D., Sekla, L., An enzyme-linked immunosorbent assay-based isolation procedure for verotoxigenic Escherichia coli. Appl Environ Microbiol 59 (1993), 4223–4229.
30. Miyajima, Y., Takahashi, M., Eguchi, H., Honma, M., Tanahashi, S., Matui, Y., et al. Outbreak of enterohemorrhagic Escherichia coli O26 in Nigata Ctiy, Japan. Jpn J Infect Dis 60 (2007), 238–239.
31. National Enteric Surveillance Program, National Enteric Surveillance Program Annual Summary. http://www.nml-lnm.gc.ca/NESP-PNSME/surveillance-2012-eng.html, 2012.
32. Ngwa, G.A., Schop, R., Weir, S., Leon-Velarde, C.G., Odumeru, J.A., Detection and enumeration of E. coli O157:H7 in water samples by culture and molecular methods. J Microbiol Methods 92 (2013), 164–172.
33. Radu, S., Rusul, G., Ling, O.W., Purwati, E., Mustakim, M., Lihan, S., Rapid isolation and detection of Escherichia coli O157:H7 by use of Rainbow agar O157™ and PCR assay. Southeast Asian J Trop Med Public Health 31 (2000), 77–79.
34. Tillman, G.E., Wasilenko, J.L., Simmons, M., Lauze, T.A., Minicozzi, J., Oakley, B.B., et al. Isolation of shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145 from ground beef using modified Rainbow agar and post-immunomagnetic separation acid treatment. J Food Prod 75 (2012), 1548–1554.
35. Tutenel, A.V., Pierard, D., Vandekerchove, D., Van Hoof, J., De Zutter, L., Sensitivity of methods for the isolation of Escherichia coli O157 from naturally infected bovine faeces. Vet Microbiol 94 (2003), 341–346.
36. Tzschoppe, M., Martin, A., Beutin, L., A rapid procedure for the detection and isolation of enterohaemorrhagic Escherichia coli (EHEC) serogroup O26, O103, O111, O118, O121, O145 and O157 strains and the aggregative EHEC O104:H4 strain from ready-to-eat vegetables. Int J Food Microbiol 152 (2012), 19–30.
37. Vojdani, J.D., Beuchat, L.R., Tauxe, R.V., Juice-associated outbreaks of human illness in the United States, 1995 through 2005. J Food Prot 71 (2008), 356–364.
38. Wylie, J.L., Van Caeseele, P., Gilmour, M.W., Sitter, D., Guttek, C., Giercke, S., Evaluation of a new chromogenic agar medium for detection of shiga toxin-producing Escherichia coli (STEC) and relative prevalence of O157 and non-O157 STEC, Manitoba, Canada. J Clin Microbiol 51 (2013), 466–471.
39. Yoshitomi, K.J., Jinneman, K.C., Zapata, R., Weagant, S.D., Fedio, W.M., Detection and isolation of low levels of E. coli O157:H7 in cilantro by real-time PCR, immunomagnetic separation, and cultural methods with and without an acid treatment. J Food Sci 77 (2012), M481–M482.