Evaluation of CHROMagar STEC and STEC o104 chromogenic agar media for detection of Shiga toxin-producing Escherichia coli in stool specimens

Journal of Clinical Microbiology

Volumn 51, Issue 3, 2013, Pages 894-900

  Gouali, Malika ^a   Ruckly, Corinne ^a   Carle, Isabelle ^a   Lejay Collin, Monique ^a   Weill, Fraņois Xavier ^a  

^a INSTITUT PASTEUR   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; BACTERIAL DNA; FLAGELLIN; O ANTIGEN; SHIGA TOXIN; SORBITOL; VEROTOXIN 1; VEROTOXIN 2;

AGGR GENE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL VIRULENCE; BACTERIUM DETECTION; CONTROLLED STUDY; CULTURE MEDIUM; DNA EXTRACTION; EAE GENE; EHEC GENE; ENRICHMENT CULTURE; ENTEROPATHOGENIC ESCHERICHIA COLI; FECES ANALYSIS; FERMENTATION; FLIC GENE; HLYA GENE; NONHUMAN; O ANTIGEN GENE; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SEROTYPE; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; STRAIN IDENTIFICATION; STX1 GENE; STX2 GENE; TOXIN ANALYSIS;

ADOLESCENT; ADULT; AGAR; BACTERIOLOGICAL TECHNIQUES; CHILD; CHILD, PRESCHOOL; CHROMOGENIC COMPOUNDS; CULTURE MEDIA; ESCHERICHIA COLI INFECTIONS; FECES; HUMANS; PREDICTIVE VALUE OF TESTS; SENSITIVITY AND SPECIFICITY; SHIGA-TOXIGENIC ESCHERICHIA COLI;

EID: 84874028803     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.03121-12     Document Type: Article

References (39)

1. Heuvelink AE, van de Kar NC, Meis JF, Monnens LA, Melchers WJ. 1995. Characterization of verocytotoxin-producing Escherichia coli O157 isolates from patients with haemolytic uraemic syndrome in Western Europe. Epidemiol. Infect. 115:1-14
2. Tarr PI, Gordon CA, Chandler WL. 2005. Shiga-toxin-producing Escherichia coli and haemolytic uraemic syndrome. Lancet 365:1073-1086
3. O'Brien AO, Lively TA, Chen ME, Rothman SW, Formal SB. 1983. Escherichia coli O157 H7 strains associated with haemorrhagic colitis in the United States produce a Shigella dysenteriae 1 (SHIGA) like cytotoxin. Lancet 1:702
4. Scheutz F, Teel LD, Beutin L, Piérard D, Buvens G, Karch H, Mellmann A, Caprioli A, Tozzoli R, Morabito S, Strockbine NA, Melton-Celsa AR, Sanchez M, Persson S, O'Brien AD. 2012. Multicenter evaluation of asequence-based protocol for subtyping Shiga toxins and standardizing Stx nomenclature. J. Clin. Microbiol. 50:2951-2963
5. Schmidt H. 2001. Shiga-toxin-converting bacteriophages. Res. Microbiol. 152:687-695
6. Trabulsi LR, Keller R, Tardelli Gomes TA. 2002. Typical and atypical enteropathogenic Escherichia coli. Emerg. Infect. Dis. 8:508-513
7. Bielaszewska M, Mellmann A, Zhang W, Köck R, Fruth A, Bauwens A, Peters G, Karch H. 2011. Characterisation of the Escherichia coli strain associated with an outbreak of haemolytic uraemic syndrome in Germany, 2011: a microbiological study. Lancet Infect. Dis. 11:671-676
8. Buchholz U, Bernard H, Werber D, Böhmer MM, Remschmidt C, Wilking H, Deleré Y, ander Heiden M, Adlhoch C, Dreesman J, Ehlers J, Ethelberg S, Faber M, Frank C, Fricke G, Greiner M, Höhle M, Ivarsson S, Jark U, Kirchner M, Koch J, Krause G, Luber P, Rosner B, Stark K, Kühne M. 2011. German outbreak of Escherichia coli O104:H4 associated with sprouts. N. Engl. J. Med. 365:1763-1770
9. Grad YH, Lipsitch M, Feldgarden M, Arachchi HM, Cerqueira GC, Fitzgerald M, Godfrey P, Haas BJ, Murphy CI, Russ C, Sykes S, Walker BJ, Wortman JR, Young S, Zeng Q, Abouelleil A, Bochicchio J, Chauvin S, Desmet T, Gujja S, McCowan C, Montmayeur A, Steelman S, Frimodt-Møller J, Petersen AM, Struve C, Krogfelt KA, Bingen E, Weill FX, Lander ES, Nusbaum C, Birren BW, Hung DT, Hanage WP. 2012. Genomic epidemiology of the Escherichia coli O104:H4 outbreaks in Europe, 2011. Proc. Natl. Acad. Sci. U. S. A. 109:3065-3070
10. King LA, Nogareda F, Weill FX, Mariani-Kurkdjian P, Loukiadis E, Gault G, Jourdan-DaSilva N, Bingen E, Macé M, Thevenot D, Ong N, Castor C, Noel H, van Cauteren D, Charron M, Vaillant V, Aldabe B, Goulet V, Delmas G, Couturier E, Le Strat Y, Combe C, Delmas Y, Terrier F, Vendrely B, Rolland P, de Valk H. 2012. Outbreak of Shiga toxin-producing Escherichia coli O104:H4 associated with organic fenugreek sprouts, France, June 2011. Clin. Infect. Dis. 54:1588-1594
11. Mariani-Kurkdjian P, Bingen E, Gault G, Jourdan-Da Silva N, Weill FX. 2011. Escherichia coli O104:H4 south-west France, June 2011. Lancet Infect. Dis. 11:732-733
12. Riley LW, Remis RS, Helgerson SD, McGee HB, Wells JG, Davis BR, Hebert RJ, Olcott ES, Johnson LM, Hargrett NT, Blake PA, Cohen ML. 1983. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N. Engl. J. Med. 308:681-685
13. Hussein HS, Bollinger LM. 2008. Influence of selective media on successful detection of Shiga toxin-producing Escherichia coli in food, fecal, and environmental samples. Foodborne Pathog. Dis. 5:227-244
14. Gunzer F, Böhm H, Rüssmann H, Bitzan M, Aleksic S, Karch H. 1992. Molecular detection of sorbitol-fermenting Escherichia coli O157 in patients with hemolytic-uremic syndrome. J. Clin. Microbiol. 30:1807-1810
15. Karch H, Böhm H, Schmidt H, Gunzer F, Aleksic S, Heesemann J. 1993. Clonal structure and pathogenicity of Shiga-like toxin-producing, sorbitolfermenting Escherichia coli O157:H. J. Clin. Microbiol. 31:1200-1205
16. Werber D, Bielaszewska M, Frank C, Stark K, Karch H. 2011. Watch out for the even eviler cousin-sorbitol-fermenting E coli O157. Lancet 377: 298-299
17. Beutin L, Krause G, Zimmermann S, Kaulfuss S, Gleier K. 2004. Characterization of Shiga toxin-producing Escherichia coli strains isolated from human patients in Germany over a 3-year period. J. Clin. Microbiol. 42:1099-1108
18. Hu J, Green D, Swoveland J, Grant M, Boyle DS. 2009. Preliminary evaluation of a procedure for improved detection of Shiga toxinproducing Escherichia coli in fecal specimens. Diagn. Microbiol. Infect. Dis. 65:21-26
19. Church DL, Emshey D, Semeniuk H, Lloyd T, Pitout JD. 2007. Evaluation of BBL CHROMagar O157 versus sorbitol-MacConkey medium for routine detection of Escherichia coli O157 in a centralized regional clinical microbiology laboratory. J. Clin. Microbiol. 45:3098-3100
20. Hirvonen JJ, Siitonen A, Kaukoranta SS. 2012. Usability and performance of CHROMagar STEC medium in detection of Shiga toxinproducing Escherichia coli strains. J. Clin. Microbiol. 50:3586-3590
21. Tzschoppe M, Martin A, Beutin L. 2012. A rapid procedure for the detection and isolation of enterohaemorrhagic Escherichia coli (EHEC) serogroup O26, O103, O111, O118, O121, O145 and O157 strains and the aggregative EHEC O104:H4 strain from ready-to-eat vegetables. Int. J. Food Microbiol. 152:19-30
22. Coimbra RS, Grimont F, Lenormand P, Burguiere P, Beutin L, Grimont PA. 2000. Identification of Escherichia coli O-serogroups by restriction of the amplified O-antigen gene cluster (rfb-RFLP). Res. Microbiol. 151:639-654
23. Machado J, Grimont F, Grimont PA. 2000. Identification of Escherichia coli flagellar types by restriction of the amplified fliC gene. Res. Microbiol. 151:535-546
24. Gunsberg ST, Tornieporth NG, Riley LW. 1995. Identification of Enteropathogenic Escherichia coli by PCR-based detection of the bundleforming pilus gene. J. Clin. Microbiol. 33:1375-1377
25. Bielaszewska M, Köck R, Friedrich AW, von Eiff C, Zimmerhackl LB, Karch H, Mellmann A. 2007. Shiga toxin-mediated hemolytic uremic syndrome: time to change the diagnostic paradigm? PLoS One 2:e1024. doi:10.1371/journal.pone. 0001024
26. Bielaszewska M, Middendorf B, Köck R, Friedrich AW, Fruth A, Karch H, Schmidt MA, Mellmann A. 2008. Shiga toxin-negative attaching and effacing Escherichia coli: distinct clinical associations with bacterial phylogeny and virulence traits and inferred in-host pathogen evolution. Clin. Infect. Dis. 47:208-217
27. Aldabe B, Delmas Y, Gault G, Vendrely B, Llanas B, Charron M, Castor C, Ong N, Weill FX, Mariani-Kurkdjian P, Terrier F, Desjardin M, Simoes J, Le Bihan B, Combe C, Rolland P. 2011. Household transmission of haemolytic uraemic syndrome associated with Escherichia coli O104:H4, south-western France, June 2011. Euro Surveill. 16(31): pii-19934. http://www.eurosurveillance.org/ ViewArticle.aspx?ArticleId-19934
28. Jourdan-da Silva N, Watrin M, Weill FX, King LA, Gouali M, Mailles A, van Cauteren D, Bataille M, Guettier S, Castrale C, Henry P, Mariani P, Vaillant V, de Valk H. 2012. Outbreak of haemolytic uraemic syndrome due to Shiga toxin-producing Escherichia coli O104:H4 among French tourists returning from Turkey, September 2011. Euro Surveill. 17(4):pii-20065. http://www. eurosurveillance.org/ViewArticle.aspx?ArticleId-20065
29. Zadik PM, Chapman PA, Siddons CA. 1993. Use of tellurite for the selection of verocytotoxigenic Escherichia coli O157. J. Med. Microbiol. 39:155-158
30. Bielaszewska M, Middendorf B, Tarr PI, Zhang W, Prager R, Aldick T, Dobrindt U, Karch H, Mellmann A. 2011. Chromosomal instability in enterohaemorrhagic Escherichia coli O157:H7: impact on adherence, tellurite resistance and colony phenotype. Mol. Microbiol. 79:1024-1044
31. Orth D, Grif K, Dierich MP, Würzner R. 2007. Variability in tellurite resistance and the ter gene cluster among Shiga toxin-producing Escherichia coli isolated from humans, animals and food. Res. Microbiol. 158: 105-111
32. Taylor DE, Rooker M, Keelan M, Ng LK, Martin I, Perna NT, Burland NT, Blattner FR. 2002. Genomic variability ofOislands encoding tellurite resistance in enterohemorrhagic Escherichia coli O157:H7 isolates. J. Bacteriol. 184:4690-4698
33. Bielaszewska M, Tarr PI, Karch H, Zhang W, Mathys W. 2005. Phenotypic and molecular analysis of tellurite resistance among enterohemorrhagic Escherichia coli O157:H7 and sorbitol-fermenting O157:NM clinical isolates. J. Clin. Microbiol. 43:452-454
34. Loukiadis E, Mariani-Kurkdjian P, Ganet S, Baliere C, Neto M, Gleizal A, Gaillot O, Spinali S, Weill FX, Bingen E, Beutin L, Thevenot-Segentet D. 2012. First isolation of sorbitol-fermenting enterohemorrhagic Escherichia coli O157 in minced beef responsible for hemolytic and uremic syndrome in France, poster P-194. Abstr. 8th Int. Symp. Shiga Toxin (Verocytotoxin) Producing E. coli Infections, VTEC 2012, Amsterdam, The Netherlands
35. Walter EG, Taylor DE. 1992. Plasmid-mediated resistance to tellurite: expressed and cryptic. Plasmid 27:52-64
36. Wylie JL, Van Caeseele P, Gilmour MW, Sitter D, Guttek C, Giercke S. 21 November 2012. Evaluation of a new chromogenic agar medium for detection of Shiga toxin-producing Escherichia coli (STEC) and relative prevalence of O157 and non-O157 STEC, Manitoba, Canada. J. Clin. Microbiol. [Epub ahead of print.] doi:10.1128/JCM.02329-12
37. Grad YH, Godfrey P, Cerquiera GC, Mariani-Kurkdjian P, Gouali M, Bingen E, Shea TP, Haas BJ, Griggs A, Young S, Zeng Q, Lipsitch M, Waldor MK, Weill F-X, Wortman JR, Hanage WP. 2013. Comparative genomics of recent Shiga toxin-producing Escherichia coli O104:H4: short-term evolution of an emerging pathogen. mBio 4(2):e00452-12. doi: 10.1128/mBio.00452-12
38. Centers for Disease Control and Prevention. 2009. Recommendations for diagnosis of Shiga-toxin producing Escherichia coli infections by clinical laboratories. MMWR Recommend. Rep. 58(RR12):1-14
39. Feng PC, Jinneman K, Scheutz F, Monday SR. 2011. Specificity of PCR and serological assays in the detection of Escherichia coli Shiga toxin subtypes. Appl. Environ. Microbiol. 77:6699-6702.