Evaluation of eight agar media for the isolation of shiga toxin-Producing Escherichia coli

Journal of Microbiological Methods

Volumn 96, Issue 1, 2014, Pages 6-11

  Gill, Alexander ^a   Huszczynski, George ^b   Gauthier, Martine ^b   Blais, Burton ^b  

^a HEALTH CANADA   (Canada)

^b CANADIAN FOOD INSPECTION AGENCY   (Canada)

Author keywords

Agar media; Escherichia coli; Growth; Selective supplements; Shiga toxin verotoxin

Indexed keywords

AGAR; CEFIXIME; EOSIN; ION; METHYLENE BLUE; TELLURITE; UNCLASSIFIED DRUG;

AGAR MEDIUM; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM ISOLATION; BEEF; CELL STRUCTURE; EAE GENE; ENRICHMENT CULTURE; GROWTH INHIBITION; HLYA GENE; HUMAN; MICROFLORA; NONHUMAN; PRIORITY JOURNAL; SEROTYPE; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; STX1 GENE; STX2 GENE;

AGAR MEDIA; ESCHERICHIA COLI; GROWTH; SELECTIVE SUPPLEMENTS; SHIGA TOXIN/VEROTOXIN;

AGAR; BACTERIOLOGICAL TECHNIQUES; CULTURE MEDIA; SELECTION, GENETIC; SHIGA-TOXIGENIC ESCHERICHIA COLI;

EID: 84887639542     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2013.10.022     Document Type: Article

References (22)

1. Atalla H.N., Johnson R., McEwen S., Usborne R.W., Gyles C.L. Use of a shiga toxin (Stx)-enzyme-linked immunosorbent assay and immunoblot for detection and isolation of stx-producing Escherichia coli from naturally contaminated beef. J. Food Prot. 2000, 63:1167-1172.
2. Barlow R.S., Gobius K.S., Desmarchelier P.M. Shiga toxin-producing Escherichia coli in ground beef and lamb cuts: results of a one-year study. Int. J. Food Microbiol. 2006, 111:1-5.
3. Bettelheim K.A. The non-O157 Shiga-toxigenic (verocytotoxigenic) Escherichia coli; under-rated pathogens. Crit. Rev. Microbiol. 2007, 33:67-87.
4. Beutin L., Martin A. Outbreak of shiga toxin-producing Escherichia coli (STEC) O104:H4 infection in Germany causes a paradigm shift with regard to human pathogenicity of STEC strains. J. Food Prot. 2012, 75:408-418.
5. Beutin L., Montenegro M.A., Orskov I., Orskov F., Prada J., Zimmermann S., Stephan R. Close association of verotoxin (Shiga-like toxin) production with enterohemolysin production in strains of Escherichia coli. J. Clin. Microbiol. 1989, 27:2559-2564.
6. CDC Foodborne Diseases Active Surveillance Network (FoodNet): FoodNet Surveillance Report for 2011 (Final Report) 2012, U.S. Department of Health and Human Services, CDC, Atlanta, Georgia.
7. Chui L., Lee M.-C., Malejczyk K., Lim L., Fok D., Kwong P. Prevalence of shiga toxin-producing Escherichia coli as detected by enzyme-linked immunoassays and real-time PCR during the summer months in Northern Alberta, Canada. J. Clin. Microbiol. 2011, 49:4307-4310.
8. European Food Safety Authority Scientific opinion of the panel on biological hazards on a request from EFSA on monitoring of verotoxigenic Escherichia coli. EFSA J. 2007, 579:1-61.
9. Gill A.O., Gill C.O. Non-O157 verotoxigenic Escherichia coli and beef: a Canadian perspective. Can. J. Vet. Res. 2010, 74:161-169.
10. Gill A., Martinez-Perez A., McIlwham S., Blais B. Development of a method for the detection of verotoxin producing Escherichia coli in food. J. Food Prot. 2012, 75:827-837.
11. Heuvelink A.E. Culture media for the isolation of diarrhoeagenic Escherichia coli from foods. Handbook of Culture Media for Food and Water Microbiology 2012, 321-356. Ch. 16. J.E.L. Corry, G.D.W. Curtis, R.M. Baird (Eds.).
12. Hiramatsu R., Matsumoto M., Miwa Y., Suzuki Y., Saito M., Miyazaki Y. Characterization of Shiga toxin-producing Escherichia coli O26 strains and establishment of selective isolation media for these strains. J. Clin. Microbiol. 2002, 40:922-925.
13. Hirvonen J.J., Siitonen A., Kaukoranta S. Usability and performance of CHROMagar STEC in detection of shiga toxin-producing Escherichia coli strains. J. Clin. Microbiol. 2012, 50:3586-3590.
14. Johnson K.E., Thorpe C.M., Sears C.L. The emerging clinical importance of non-O157 Shiga toxin-producing Escherichia coli. Clin. Infect. Dis. 2006, 43:1587-1595.
15. Kaper J.B., Nataro J.P., Mobley H.L. Pathogenic Escherichia coli. Nat. Rev. Microbiol. 2004, 2:123-140.
16. Melton-Celsa A., Mohawk K., Teel L., O'Brien A. Pathogenesis of shiga-toxin producing Escherichia coli. Curr. Top. Microbiol. Immunol. 2012, 357:67-103.
17. Paton A.W., Paton J.C. Detection and characterization of STEC in stool samples using PCR. An overview. Methods Mol. Med. 2003, 73:45-54.
18. Possé B., De Zutter L., Heyndrickx M., Herman L. Novel differential and confirmation plating media for Shiga toxin-producing Escherichia coli serotypes O26, O103, O111, O145 and sorbitol positive and negative O157. FEMS Microbiol. Lett. 2008, 282:124-131.
19. Public Health Agency of Canada National enteric surveillance program (NESP). Annual Summary 2010 2010, (http://www.nml-lnm.gc.ca/NESP-PNSME/surveillance-2010-eng.html. Accessed 11 March 2013).
20. Thompson L.H., Giercke S., Beaudoin C., Woodward D., Wylie J.L. Enhanced surveillance of non-O157 verotoxin-producing Escherichia coli in human stool samples from Manitoba. Can. J. Infect. Dis. Med. Microbiol. 2005, 16:329-334.
21. Tzschoppe M., Martin A., Beutin L. A rapid procedure for the detection and isolation of enterohaemorrhagic Escherichia coli (EHEC) serogroup O26, O103, O111, O118, O121, O145 and O157 strains and the aggregative EHEC O104:H4 strain from ready-to-eat vegetables. Int. J. Food Microbiol. 2012, 152:19-30.
22. USDA MLG 5B.03 detection and isolation of non-O157 shiga toxin-producing Escherichia coli (STEC) from meat products (Accessed 17 March 2013). http://www.fsis.usda.gov/PDF/MLG-5B.pdf.