A rapid procedure for the detection and isolation of enterohaemorrhagic Escherichia coli (EHEC) serogroup O26, O103, O111, O118, O121, O145 and O157 strains and the aggregative EHEC O104:H4 strain from ready-to-eat vegetables

International Journal of Food Microbiology

Volumn 152, Issue 1-2, 2012, Pages 19-30

  Tzschoppe, Markus ^a   Martin, Annett ^b   Beutin, Lothar ^a  

^a Unit 41: Microbial Toxins   (Germany)

^b FEDERAL INSTITUTE FOR RISK ASSESSMENT   (Germany)

Author keywords

Chromogenic media; Detection; E. coli O104:H4; Enterohaemorrhagic E. coli (EHEC); Real time PCR; Vegetables

Indexed keywords

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIAL TRANSMISSION; BACTERIUM CONTAMINATION; BACTERIUM DETECTION; BACTERIUM ISOLATION; ENTEROHEMORRHAGIC ESCHERICHIA COLI; EPIDEMIC; ESCHERICHIA COLI O157; FOOD ANALYSIS; FOOD CONTAMINATION; GERMANY; NONHUMAN; PLANT SEED; POLYMERASE CHAIN REACTION; RAW FOOD; SPROUT; VEGETABLE; ANIMAL; CLASSIFICATION; ESCHERICHIA COLI INFECTION; EVALUATION; FOOD CONTROL; FOOD PACKAGING; GENETICS; HEMOLYTIC UREMIC SYNDROME; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ENTEROBACTERIACEAE; ESCHERICHIA COLI; PSEUDOMONAS;

O ANTIGEN;

ANIMALS; ENTEROHEMORRHAGIC ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; FOOD MICROBIOLOGY; FOOD PACKAGING; GERMANY; HEMOLYTIC-UREMIC SYNDROME; HUMANS; O ANTIGENS; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VEGETABLES;

EID: 84857133963     PISSN: 01681605     EISSN: 18793460     Source Type: Journal    
DOI: 10.1016/j.ijfoodmicro.2011.10.009     Document Type: Article

References (48)

1. Anonymous Untersuchung von Lebensmitteln: Nachweis, Isolierung und Charakterisierung Verotoxin-bildender Escherichia coli (VTEC) in Hackfleisch mittels PCR und DNA-Hybridisierungstechnik. Amtliche Sammlung für Untersuchungsverfahren nach 2002, §64:1-12. LFGB 07.18/1.
2. Anonymous Untersuchung von Lebensmitteln: Nachweis von Verotoxin-bildenden Escherichia coli Stämmen (VTEC) in Lebensmitteln tierischer Herkunft. Amtliche Sammlung für Untersuchungsverfahren nach 2006, §64:1-18. LFGB 00.00/92.
3. Anonymous EHEC O104:H4. Aktualisierte Hinweise und Hilfestellungen des RKI zur Diagnostik des gegenwärtig zirkulierenden Ausbruchstammes 2011, Robert Koch Institut, Berlin, Germany, http://www.rki.de/cln_153/nn_217400/EN/Home/EHECO104,templateId=raw,property=publicationFile.pdf/EHECO104.pdf.
4. Berger C.N., Sodha S.V., Shaw R.K., Griffin P.M., Pink D., Hand P., Frankel G. Fresh fruit and vegetables as vehicles for the transmission of human pathogens. Environmental Microbiology 2010, 12:2385-2397.
5. Bettelheim K.A., Beutin L. Rapid laboratory identification and characterization of verocytotoxigenic (Shiga toxin producing) Escherichia coli (VTEC/STEC). Journal of Applied Microbiology 2003, 95:205-217.
6. Beutin L., Jahn S., Fach P. Evaluation of the 'GeneDisc' real-time PCR system for detection of enterohaemorrhagic Escherichia coli (EHEC) O26, O103, O111, O145 and O157 strains according to their virulence markers and their O- and H-antigen-associated genes. Journal of Applied Microbiology 2009, 106:1122-1132.
7. Beutin L., Kaulfuss S., Herold S., Oswald E., Schmidt H. Genetic analysis of enteropathogenic and enterohemorrhagic Escherichia coli serogroup O103 strains by molecular typing of virulence and housekeeping genes and pulsed-field gel electrophoresis. Journal of Clinical Microbiology 2005, 43:1552-1563.
8. Beutin L., Krause G., Zimmermann S., Kaulfuss S., Gleier K. Characterization of Shiga toxin-producing Escherichia coli strains isolated from human patients in Germany over a 3-year period. Journal of Clinical Microbiology 2004, 42:1099-1108.
9. Bielaszewska M., Mellmann A., Zhang W., Kock R., Fruth A., Bauwens A., Peters G., Karch H. Characterisation of the Escherichia coli strain associated with an outbreak of haemolytic uraemic syndrome in Germany, 2011: a microbiological study. The Lancet Infectious Diseases 2011, 11:671-676.
10. Brooks J.T., Sowers E.G., Wells J.G., Greene K.D., Griffin P.M., Hoekstra R.M., Strockbine N.A. Non-O157 Shiga toxin-producing Escherichia coli infections in the United States, 1983-2002. The Journal of Infectious Diseases 2005, 192:1422-1429.
11. Bugarel M., Beutin L., Martin A., Gill A., Fach P. Micro-array for the identification of Shiga toxin-producing Escherichia coli (STEC) seropathotypes associated with Hemorrhagic Colitis and Hemolytic Uremic Syndrome in humans. International Journal of Food Microbiology 2010, 142:318-329.
12. Bugarel M., Beutin L., Scheutz F., Loukiadis E., Fach P. Identification of genetic markers for differentiation of Shiga toxin-producing, enteropathogenic and avirulent strains of Escherichia coli O26. Applied and Environmental Microbiology 2011, 76:203-211.
13. Bugarel M., Martin A., Fach P., Beutin L. Virulence gene profiling of enterohemorrhagic (EHEC) and enteropathogenic (EPEC) Escherichia coli strains: a basis for molecular risk assessment of typical and atypical EPEC strains. BMC Microbiology 2011, 11:142.
14. Caprioli A., Morabito S., Brugèreb H., Oswald E. Enterohaemorrhagic Escherichia coli: emerging issues on virulence and modes of transmission. Veterinary Research 2005, 36:289-311.
15. Cassar C.A., Ottaway M., Paiba G.A., Futter R., Newbould S., Woodward M.J. Absence of enteroaggregative Escherichia coli in farmed animals in Great Britain. The Veterinary Record 2004, 154:237-239.
16. Centers for Disease Control and Prevention FoodNet 2007 Suveillance Report 2009, CDC, Atlanta, US.
17. Cooley M.B., Miller W.G., Mandrell R.E. Colonization of Arabidopsis thaliana with Salmonella enterica and enterohemorrhagic Escherichia coli O157:H7 and competition by Enterobacter asburiae. Applied and Environmental Microbiology 2003, 69:4915-4926.
18. De Boer B.E., Heuvelink A.E. Methods for the detection and isolation of Shiga toxin-producing Escherichia coli. Symposium Series Society for Applied Microbiology 2000, 133S-143S.
19. DGHM working group Veröffentlichte mikrobiologische Richt- und Warnwerte zur Beurteilung von Lebensmitte ln. Hannover: Deutsche Gesellschaft für Hygiene und Mikrobiologie (DGHM), Fachgruppe Lebensmittelmikrobiologie und -hygiene 2010.
20. EFSA working group Technical specifications for the monitoring and reporting of verotoxigenic Escherichia coli (VTEC) on animals and food (VTEC surveys on animals and food). The EFSA Journal 2009, 7:1366.
21. EFSA working group (2010) Trends and Sources of Zoonoses and Zoonotic Agents and Food-borne outbreaks in the European Union in 2008. ed. European Food Safety Authority pp. 1496.
22. Erickson M.C., Doyle M.P. Food as a vehicle for transmission of Shiga toxin-producing Escherichia coli. Journal of Food Protection 2007, 70:2426-2449.
23. European Food Safety Authority Urgent advice on the public health risk of Shiga-toxin producing Escherichia coli in fresh vegetables. The EFSA Journal 2011, 9:2274.
24. European Food Safety Authority (EFSA) The community summary report on food-borne outbreaks in the European Union in 2007. The EFSA Journal 2009, 1-128.
25. Feng P.C., Councell T., Keys C., Monday S.R. Virulence characterization of Shiga-toxigenic Escherichia coli isolates from wholesale produce. Applied and Environmental Microbiology 2011, 77:343-345.
26. Frank C., Faber M., Askar M., Bernard H., Fruth A., Gilsdorf A., Hohle M., Karch H., Krause G., Prager R., Spode A., Stark K., Werber D. Large and ongoing outbreak of haemolytic uraemic syndrome, Germany, May 2011. Euro Surveillance 2011, 16.
27. Franz E., van Bruggen A.H. Ecology of E. coli O157:H7 and Salmonella enterica in the primary vegetable production chain. Critical Review Microbiology 2008, 34:143-161.
28. Fremaux B., Prigent-Combaret C., Vernozy-Rozand C. Long-term survival of Shiga toxin-producing Escherichia coli in cattle effluents and environment: an updated review. Veterinary Microbiology 2008, 132:1-18.
29. Gourabathini P., Brandl M.T., Redding K.S., Gunderson J.H., Berk S.G. Interactions between food-borne pathogens and protozoa isolated from lettuce and spinach. Applied and Environmental Microbiology 2008, 74:2518-2525.
30. Hussein H.S., Bollinger L.M. Influence of selective media on successful detection of Shiga toxin-producing Escherichia coli in food, fecal, and environmental samples. Foodborne Pathogenesis Disease 2008, 5:227-244.
31. Jahn S., Beutin L. Evaluation of the "GeneDisc" Real-Time PCR System for Detection of Major EHEC Type Strains from Different Food Matrices (minced meat, "Mettwurst", salami and raw milk) 2009, Buenos Aires, Argentina, VTEC, 2009, book of abstracts.
32. Karmali M.A., Mascarenhas M., Shen S., Ziebell K., Johnson S., Reid-Smith R., Isaac-Renton J., Clark C., Rahn K., Kaper J.B. Association of genomic O island 122 of Escherichia coli EDL 933 with verocytotoxin-producing Escherichia coli seropathotypes that Are linked to epidemic and/or serious disease. Journal of Clinical Microbiology 2003, 41:4930-4940.
33. Klepzig I., Teufel P., Schott W., Hildebrandt G. Auswirkungen einer Unterbrechung der Kühlkette auf die mikrobiologische Beschaffenheit von vorzerkleinerten Mischsalaten. Archiv für Lebensmittelhygiene 1999, 50:95-104.
34. Lack W.K., Becker B., Holzapfel W.H. Hygienischer Status frischer vorverpackter Mischsalate im Jahr 1995. Archiv für Lebensmittelhygiene 1996, 47:129-152.
35. Maidhof H., Guerra B., Abbas S., Elsheikha H.M., Whittam T.S., Beutin L. A multiresistant clone of Shiga toxin-producing Escherichia coli O118:[H16] is spread in cattle and humans over different European countries. Applied and Environmental Microbiology 2002, 68:5834-5842.
36. Martin A., Beutin L. Characteristics of Shiga toxin-producing Escherichia coli from meat and milk products of different origins and association with food producing animals as main contamination sources. International Journal of Food Microbiology 2011, 146:99-104.
37. Mead P.S., Slutsker L., Dietz V., McCaig L.F., Bresee J.S., Shapiro C., Griffin P.M., Tauxe R.V. Food-related illness and death in the United States. Emerging Infectious Diseases 1999, 5:607-625.
38. Nataro J.P., Kaper J.B. Diarrheagenic Escherichia coli. Clinical Microbiology Reviews 1998, 11:142-201.
39. Nataro J.P., Steiner T., Guerrant R.L. Enteroaggregative Escherichia coli. Emerging Infectious Diseases 1998, 4:251-261.
40. Nataro J.P., Yikang D., Giron J.A., Savarino S.J., Kothary M.H., Hall R. Aggregative adherence fimbria I expression in enteroaggregative Escherichia coli requires two unlinked plasmid regions. Infection and Immunity 1993, 61:1126-1131.
41. Orth D., Grif K., Dierich M.P., Wurzner R. Variability in tellurite resistance and the ter gene cluster among Shiga toxin-producing Escherichia coli isolated from humans, animals and food. Research in Microbiology 2007, 158:105-111.
42. Rangel J.M., Sparling P.H., Crowe C., Griffin P.M., Swerdlow D.L. Epidemiology of Escherichia coli O157: H7 outbreaks, United States, 1982-2002. Emerging Infectious Diseases 2005, 11:603-609.
43. Sagoo S.K., Little C.L., Ward L., Gillespie I.A., Mitchell R.T. Microbiological study of ready-to-eat salad vegetables from retail establishments uncovers a national outbreak of salmonellosis. Journal of Food Protection 2003, 66:403-409.
44. Scheutz F., Strockbine N.A. Genus I. Escherichia. Bergey's Manual of Systematic Bacteriology 2005, 607-624. Springer. G.M. Garrity, D.J. Brenner, N.R. Krieg, J.T. Staley (Eds.).
45. Tarr C.L., Large T.M., Moeller C.L., Lacher D.W., Tarr P.I., Acheson D.W., Whittam T.S. Molecular characterization of a serotype O121:H19 clone, a distinct Shiga toxin-producing clone of pathogenic Escherichia coli. Infection and Immunity 2002, 70:6853-6859.
46. Taylor D.E., Rooker M., Keelan M., Ng L.K., Martin I., Perna N.T., Burland N.T., Blattner F.R. Genomic variability of O islands encoding tellurite resistance in enterohemorrhagic Escherichia coli O157:H7 isolates. Journal of Bacteriology 2002, 184:4690-4698.
47. Uber A.P., Trabulsi L.R., Irino K., Beutin L., Ghilardi A.C., Gomes T.A., Liberatore A.M., de Castro A.F., Elias W.P. Enteroaggregative Escherichia coli from humans and animals differ in major phenotypical traits and virulence genes. FEMS Microbiology Letters 2006, 256:251-257.
48. USDA working group (2010) Detection and isolation of non-O157 Shiga-toxin Producing Escherichia coli strains (STEC) from Meat Products. In Laboratory Guidebook ed. LQAD pp. 1-16. Athens: USDA, Laboratory QA/QC Division.