PCR detection of Escherichia coli O157:H7 directly from stools: Evaluation of commercial extraction methods for purifying fecal DNA

Journal of Clinical Microbiology

Volumn 38, Issue 11, 2000, Pages 4108-4113

  Holland, J L ^a   Louie, L ^a   Simor, A E ^a   Louie, M ^a  

^a SUNNYBROOK RESEARCH INSTITUTE   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

ANALYTIC METHOD; ARTICLE; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; ESCHERICHIA COLI; FECES ANALYSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0034462426     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.38.11.4108-4113.2000     Document Type: Article

References (34)

1. Capacity of nine thermostable DNA polymerases to mediate DNA amplification in the presence of PCR-inhibiting samples
2. Polymerase chain reaction for diagnosis of enterohemorrhagic Escherichia coli infection and hemolytic-uremic syndrome
3. Improved detection of enterotoxigenic Escherichia coli among patients with travelers' diarrhea, by the use of the polymerase chain reaction technique
4. Reliability of PCR directly from stool samples: Usefulness of an internal standard
5. Fast and reliable extraction of protozoan parasite DNA from fecal specimens
6. The effects of plant polysaccharides and buffer additives on PCR
7. Evaluation of an immunoassay for direct detection of Escherichia coli O157 in stool specimens
8. The determination of the primary structure of the 16S ribosomal RNA of Escherichia coli. 2. Nucleotide sequences of products from partial enzymatic hydrolysis
9. Application of polymerase chain reaction of the diagnosis of infectious diseases
10. Carrier RNA enhancement of recovery of DNA from dilute solutions
11. Rapid and sensitive method for detection of Shiga-like toxin-producing Escherichia coli in ground beef using the polymerase chain reaction
12. Detection of Helicobacter pylori DNA in fecal samples from infected individuals
13. Specific detection of toxigenic strains of Clostridium difficile in stool specimens
14. Detection of Escherichia coli heat-stable enterotoxin genes in pig stool specimens by an immobilized, colorimetric, nested polymerase chain reaction
15. Polymerase chain reaction-gene probe detection system specific for pathogenic strains of Yersinia enterocolitica
16. Infection by verocytotoxin-producing Escherichia coli
17. Detection of toxigenic Clostridium difficile in stool specimens by the polymerase chain reaction
18. Comparison of three stool-processing methods for detection of Salmonella serogroups B, C2, and D by PCR
19. Removal of PCR inhibitors from human faecal samples through the use of an aqueous two-phase system for sample preparation prior to PCR
20. Detection of campylobacter in gastroenteritis: Comparison of direct PCR assay of faecal samples with selective culture
21. Rapid and effective method for preparation of fecal specimens for PCR assays
22. Sequence heterogeneity of the eae gene and detection of verotoxin-producing Escherichia coli using serotype-specific primers
23. Application of multiplex PCR for detection of non-O157 verocytotoxin-producing Escherichia coli in bloody stools: Identification of serogroups O26 and O111
24. Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model
25. Comparison of sorbitol MacConkey agar and a two-step method which utilizes enzyme-linked immunosorbent assay toxin testing and a chromogenic agar to detect and isolate enterohemorrhagic Escherichia coli
26. Detection of Bacteroides fragilis enterotoxin gene by PCR
27. A simple method for diagnosing M. tuberculosis infection in clinical samples using PCR
28. Multiplex PCR assay and simple preparation method for stool specimens detect enterotoxigenic Escherichia coli DNA during course of infection
29. Evaluation of DNA preparation techniques for detection of the SLT-1 gene of Escherichia coli O157:H7 in bovine faeces using the polymerase chain reaction
30. Escherichia coli O157:H7: Clinical, diagnostic, and epidemiological aspects of human infection
31. The detection of vero cytotoxin-producing Escherichia coli and Shigella dysenteriae type 1 in faecal specimens using polymerase chain reaction gene amplification
32. The magnetic immuno polymerase chain reaction assay for direct detection of salmonellae in fecal specimens
33. Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions
34. Rapid and sensitive assay for the detection of enterotoxigenic Bacteroides fragilis