Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells

Methods

Volumn 101, Issue , 2016, Pages 113-124

  Ho, Seok Man ^a   Hartley, Brigham J ^a   TCW, Julia ^a   Beaumont, Michael ^a   Stafford, Khalifa ^a   Slesinger, Paul A ^a   Brennand, Kristen J ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

Directed differentiation; hiPSC; INeuron; Modeling neuropsychiatric disease; Neuronal induction

Indexed keywords

GLIAL FIBRILLARY ACIDIC PROTEIN; GLUTAMATE DECARBOXYLASE 67; LENTIVIRUS VECTOR; NEUROGENIN 2; PROTEIN S100B; SYNAPSIN I; TRYPTOPHAN HYDROXYLASE; VESICULAR INHIBITORY AMINO ACID TRANSPORTER; BASIC HELIX LOOP HELIX TRANSCRIPTION FACTOR; NERVE PROTEIN; NEUROG2 PROTEIN, HUMAN;

ARTICLE; ASTROCYTE; CELL MATURATION; CONTROLLED STUDY; DOPAMINERGIC NERVE CELL; HUMAN; HUMAN CELL; INDUCED PLURIPOTENT STEM CELL; NERVE CELL DIFFERENTIATION; NEURAL STEM CELL; PRIORITY JOURNAL; SYNAPTOGENESIS; CELL CULTURE; CELL CULTURE TECHNIQUE; GENE EXPRESSION; LENTIVIRUS; NUCLEAR REPROGRAMMING; PHYSIOLOGY; TRANSCRIPTION INITIATION;

BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CELLULAR REPROGRAMMING; GENE EXPRESSION; HUMANS; INDUCED PLURIPOTENT STEM CELLS; LENTIVIRUS; NERVE TISSUE PROTEINS; NEURAL STEM CELLS; TRANSCRIPTIONAL ACTIVATION;

EID: 84964790917     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2015.11.019     Document Type: Article

References (39)

1. Molyneaux B.J., Arlotta P., Menezes J.R., Macklis J.D. Neuronal subtype specification in the cerebral cortex. Nat. Rev. Neurosci. 2007, 8:427-437.
2. Kamiya A., et al. A schizophrenia-associated mutation of DISC1 perturbs cerebral cortex development. Nat. Cell Biol. 2005, 7:1167-1178.
3. Kvajo M., et al. A mutation in mouse Disc1 that models a schizophrenia risk allele leads to specific alterations in neuronal architecture and cognition. Proc. Natl. Acad. Sci. U.S.A. 2008, 105:7076-7081.
4. Black J.E., et al. Pathology of layer V pyramidal neurons in the prefrontal cortex of patients with schizophrenia. Am. J. Psychiatry 2004, 161:742-744.
5. Glantz L.A., Lewis D.A. Decreased dendritic spine density on prefrontal cortical pyramidal neurons in schizophrenia. Arch. Gen. Psychiatry 2000, 57:65-73.
6. Pletnikov, et al. Inducible expression of mutant human DISC1 in mice is associated with brain and behavioral abnormalities reminiscent of schizophrenia. Mol. Psychiatry 2007, 13:173-186.
7. Brennand K.J., Landek-Salgado M.A., Sawa A. Modeling heterogeneous patients with a clinical diagnosis of schizophrenia with induced pluripotent stem cells. Biol. Psychiatry 2014, 75:936-944.
8. Chambers S.M., et al. Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat. Biotechnol. 2009, 27:275-280.
9. Muotri A.R., et al. L1 retrotransposition in neurons is modulated by MeCP2. Nature 2010, 468:443-446.
10. Brennand K.J., et al. Modelling schizophrenia using human induced pluripotent stem cells. Nature 2011, 473:221-225.
11. Marchetto M., et al. A model for neural development and treatment of Rett syndrome using human induced pluripotent stem cells. Cell 2010, 143:527-539.
12. Nicholas C., et al. Functional maturation of hPSC-derived forebrain interneurons requires an extended timeline and mimics human neural development. Cell Stem Cell 2013, 12:573-586.
13. Brennand, et al. Phenotypic differences in hiPSC NPCs derived from patients with schizophrenia. Mol. Psychiatry 2014, 20(3):361-368.
14. Mariani J., et al. Modeling human cortical development in vitro using induced pluripotent stem cells. Proc. Natl. Acad. Sci. U.S.A. 2012, 109:12770-12775.
15. Shi Y., Kirwan P., Smith J., Robinson H.P., Livesey F.J. Human cerebral cortex development from pluripotent stem cells to functional excitatory synapses. Nat. Neurosci. 2012, 15:477-486. S1.
16. Espuny-Camacho I., et al. Pyramidal neurons derived from human pluripotent stem cells integrate efficiently into mouse brain circuits in vivo. Neuron 2013, 77:440-456.
17. Pang Z.P., et al. Induction of human neuronal cells by defined transcription factors. Nature 2011, 476:220-223.
18. Yoo A., et al. MicroRNA-mediated conversion of human fibroblasts to neurons. Nature 2011, 476:228-231.
19. Zhang Y., et al. Rapid single-step induction of functional neurons from human pluripotent stem cells. Neuron 2013, 78:785-798.
20. Chanda S., et al. Generation of induced neuronal cells by the single reprogramming factor ASCL1. Stem Cell Rep. 2014, 10.1016/j.stemcr.2014.05.020.
21. Busskamp V., et al. Rapid neurogenesis through transcriptional activation in human stem cells. Mol. Syst. Biol. 2014, 10.
22. Yuan L., Hassan B.A. Neurogenins in brain development and disease: an overview. Arch. Biochem. Biophys. 2014, 558:10-13.
23. Mizuguchi R., et al. Combinatorial roles of olig2 and neurogenin2 in the coordinated induction of pan-neuronal and subtype-specific properties of motoneurons. Neuron 2001, 31(5):757-771.
24. Sun Y., et al. Neurogenin promotes neurogenesis and inhibits glial differentiation by independent mechanisms. Cell 2001, 104(3):365-376.
25. Ross S.E., et al. Basic helix-loop-helix factors in cortical development. Neuron 2003, 39(1):13-25.
26. Heinrich C., et al. Directing astroglia from the cerebral cortex into subtype specific functional neurons. PLoS Biol. 2010, 8:e1000373.
27. Thoma E.C., et al. Ectopic expression of neurogenin 2 alone is sufficient to induce differentiation of embryonic stem cells into mature neurons. PLoS One 2012, 7(6):e38651. 10.1371/journal.pone.0038651.
28. Lee S.K., et al. Synchronization of neurogenesis and motor neuron specification by direct coupling of bHLH and homeodomain transcription factors. Neuron 2003, 38(5):731-745.
29. Ma Y.C., et al. Regulation of motor neuron specification by phosphorylation of neurogenin 2. Neuron 2008, 58(1):65-77.
30. Hester M.E., et al. Rapid and efficient generation of functional motor neurons from human pluripotent stem cells using gene delivered transcription factor codes. Mol. Ther. 2011, 19(10):1905-1912.
31. Liu M.L., et al. Small molecules enable neurogenin 2 to efficiently convert human fibroblasts into cholinergic neurons. Nat. Commun. 2013, 4.
32. Roybon L., et al. GABAergic differentiation induced by Mash1 is compromised by the bHLH proteins Neurogenin2, NeuroD1, and NeuroD2. Cereb. Cortex 2010, 20(5):1234-1244.
33. Topol A., Tran N.N., Brennand K.J. A guide to generating and using hiPSC derived NPCs for the study of neurological diseases. J. Vis. Exp.: JoVE 2015, 96:e52495.
34. Yu X., Dillon G.P., Bellamkonda R.V. A laminin and nerve growth factor-laden three-dimensional scaffold for enhanced neurite extension. Tissue Eng. 1999, 5(4):291-304.
35. Tiscornia G., Singer O., Verma I.M. Production and purification of lentiviral vectors. Nat. Protoc. Electron. Ed. 2006, 1:241.
36. O'Doherty U., et al. Human immunodeficiency virus type 1 spinoculation enhances infection through virus binding. J. Virol. 2000, 74(21):10074-10080.
37. Beaudoin G.M., et al. Culturing pyramidal neurons from the early postnatal mouse hippocampus and cortex. Nat. Protoc. 2012, 7(9):1741-1754.
38. Sequerra E., Costa M., Menezes J., Hedin-Pereira C. Adult neural stem cells: plastic or restricted neuronal fates. Development (Cambridge, England) 2013, 140:3303-3309.
39. Miller J.A., et al. Transcriptional landscape of the prenatal human brain. Nature 2014, 508:199-206.