Efficient Generation of Myostatin Gene Mutated Rabbit by CRISPR/Cas9

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Lv, Qingyan ^a   Yuan, Lin ^a   Deng, Jichao ^a   Chen, Mao ^a   Wang, Yong ^a   Li, Zhanjun ^a   Lai, Liangxue ^a,b  

^a JILIN UNIVERSITY   (China)

^b GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH   (China)

Author keywords

[No Author keywords available]

Indexed keywords

MYOSTATIN;

ANIMAL; ANIMAL MODEL; CRISPR CAS SYSTEM; GENE KNOCKOUT; GENETICS; HYPERPLASIA; HYPERTROPHY; LEPORIDAE; METABOLISM; MUSCLE; MUTATION; PATHOLOGY; PHENOTYPE;

ANIMALS; CRISPR-CAS SYSTEMS; GENE KNOCKOUT TECHNIQUES; HYPERPLASIA; HYPERTROPHY; MODELS, ANIMAL; MUSCLES; MUTATION; MYOSTATIN; PHENOTYPE; RABBITS;

EID: 84964692641     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep25029     Document Type: Article

References (26)

1. Horii, T. et al. Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering. Sci. Rep. 4, 4513, doi: 10.1038/srep04513 (2014).
2. Crispo, M. et al. Efficient generation of myostatin knock-out sheep using CRISPR/Cas9 technology and microinjection into zygotes. Plos one 10, e0136690 (2015).
3. Kang, Y. et al. CRISPR/Cas9-mediated Dax1 knockout in the monkey recapitulates human AHC-HH. Hum. Mol. Genet ddv425, doi: 10.1093/hmg/ddv425 (2015).
4. Wang, Y. et al. Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA. Sci. Rep. 5, 8256, doi: 10.1038/ srep08256 (2015).
5. Wang, Y. et al. Generation of knockout rabbits using transcription activator-like effector nucleases. Cell. Regen. 3, 1-9 (2014).
6. Wang, Y. et al. Human apolipoprotein A-II protects against diet-induced atherosclerosis in transgenic rabbits. Arterioscl. Throm. Vas. 33, 224-231 (2013).
7. Kondo, M. et al. Generation of a transgenic rabbit model of retinal degeneration. Invest. Ophth. Vis. Sci. 50, 1371 (2009).
8. Lee, S.-J. Regulation of muscle mass by myostatin. Annu. Rev. Cell Dev. Biol. 20, 61-86 (2004).
9. McPherron, A. C., Lawler, A. M. & Lee, S.-J. Regulation of skeletal muscle mass in mice by a new TGF-p superfamily member. Nature 387, 83-90 (1997).
10. Fries, R., Hanset, R. & Georges, M. A deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle. Nat. Genet. 17, 71 (1997).
11. Clop, A. et al. A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep. Nat. Genet. 38, 813-818 (2006).
12. Qian, L. et al. Targeted mutations in myostatin by zinc-finger nucleases result in double-muscled phenotype in Meishan pigs. Sci. Rep. 5, 14435, doi: 10.1038/srep14435 (2015).
13. Zou, Q. et al. Generation of gene-target dogs using CRISPR/Cas9 system. J. Mol. Cell. Biol. 7, 580-583 (2015).
14. Zhou, F., Sun, R., Chen, H., Fei, J. & Lu, D. Myostatin Gene Mutated Mice Induced with TALE Nucleases. Anim. Biotechnol. 26, 169-179 (2015).
15. Ni, W. et al. Efficient gene knockout in goats using CRISPR/Cas9 system. Plos one 9, e106718 (2014).
16. Zhou, J. et al. Dual sgRNAs facilitate CRISPR/Cas9 mediated mouse genome targeting. FEBS. J. 281, 1717-1725 (2014).
17. Pattanayak, V. et al. High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nat. Biotechnol. 31, 839-843 (2013).
18. Hsu, P. D. et al. DNA targeting specificity of RNA-guided Cas9 nucleases. Nat. Biotechnol. 31, 827-832 (2013).
19. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat. Biotechnol. 31, 822-826 (2013).
20. Wu, X. et al. Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells. Nat. Biotechnol. 32, 670-676 (2014).
21. Ran, F. A. et al. Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Cell 154, 1380-1389 (2013).
22. Guilinger, J. P., Thompson, D. B. & Liu, D. R. Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification. Nat. Biotechnol. 32, 577-582 (2014).
23. Arthur, P. Double muscling in cattle: a review. Crop. Pasture. Sci. 46, 1493-1515 (1995).
24. Mosher, D. S. et al. A mutation in the myostatin gene increases muscle mass and enhances racing performance in heterozygote dogs. Plos Genet 3, e79 (2007).
25. Wegner, J. et al. Growth-and breed-related changes of muscle fiber characteristics in cattle. J. Anim. Sci. 78, 1485-1496 (2000).
26. Guschin, D. Y. et al. A rapid and general assay for monitoring endogenous gene modification. Methods Mol. Biol. 649, 247-256 (2010).