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Volumn 64, Issue 14, 2016, Pages 2917-2924

Structural Dissection of the Active Site of Thermotoga maritima β-Galactosidase Identifies Key Residues for Transglycosylating Activity

Author keywords

enzyme engineering; galacto oligosaccharides; GH2 glycoside hydrolase; prebiotics

Indexed keywords

BIOCHEMISTRY; ENZYME ACTIVITY; ENZYMES; OLIGOSACCHARIDES; PLANTS (BOTANY); SUGARS;

EID: 84964595351     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/acs.jafc.6b00222     Document Type: Article
Times cited : (24)

References (46)
  • 1
    • 64549134658 scopus 로고    scopus 로고
    • In vitro fermentation profiles, gas production rates, and microbiota modulation as affected by certain fructans, galactooligosaccharides, and Polydextrose
    • Hernot, D. C.; Boileau, T. W.; Bauer, L. L.; Middelbos, I. S.; Murphy, M. R.; Swanson, K. S.; Fahey, G. C., Jr. In vitro fermentation profiles, gas production rates, and microbiota modulation as affected by certain fructans, galactooligosaccharides, and Polydextrose J. Agric. Food Chem. 2009, 57, 1354-1361 10.1021/jf802484j
    • (2009) J. Agric. Food Chem. , vol.57 , pp. 1354-1361
    • Hernot, D.C.1    Boileau, T.W.2    Bauer, L.L.3    Middelbos, I.S.4    Murphy, M.R.5    Swanson, K.S.6    Fahey, G.C.7
  • 2
    • 70349748450 scopus 로고    scopus 로고
    • Protective effect of galactooligosaccharide-supplemented enteral nutrition on intestinal barrier function in rats with severe acute pancreatitis
    • Zhong, Y.; Cai, D.; Cai, W.; Geng, S.; Chen, L.; Han, T. Protective effect of galactooligosaccharide-supplemented enteral nutrition on intestinal barrier function in rats with severe acute pancreatitis Clin. Nutr. 2009, 28, 575-580 10.1016/j.clnu.2009.04.026
    • (2009) Clin. Nutr. , vol.28 , pp. 575-580
    • Zhong, Y.1    Cai, D.2    Cai, W.3    Geng, S.4    Chen, L.5    Han, T.6
  • 3
    • 38349181488 scopus 로고    scopus 로고
    • Bacterial metabolism and health-related effects of galacto-oligosaccharides and other prebiotics
    • Macfarlane, G. T.; Steed, H.; Macfarlane, S. Bacterial metabolism and health-related effects of galacto-oligosaccharides and other prebiotics J. Appl. Microbiol. 2008, 104, 305-344 10.1111/j.1365-2672.2007.03520.x
    • (2008) J. Appl. Microbiol. , vol.104 , pp. 305-344
    • Macfarlane, G.T.1    Steed, H.2    Macfarlane, S.3
  • 4
    • 33947136322 scopus 로고    scopus 로고
    • Prebiotics: The concept revisited
    • Roberfroid, M. Prebiotics: the concept revisited J. Nutr. 2007, 137, 830S-837S
    • (2007) J. Nutr. , vol.137 , pp. 830S-837S
    • Roberfroid, M.1
  • 5
    • 77956408763 scopus 로고    scopus 로고
    • Galacto-oligosaccharides: Production, properties, applications, and significance as prebiotics
    • Torres, D. P. M.; Goncalves, M. D. P.; Teixeira, J. A.; Rodrigues, L. G. R. Galacto-oligosaccharides: production, properties, applications, and significance as prebiotics Compr. Rev. Food Sci. Food Saf. 2010, 9, 438-454 10.1111/j.1541-4337.2010.00119.x
    • (2010) Compr. Rev. Food Sci. Food Saf. , vol.9 , pp. 438-454
    • Torres, D.P.M.1    Goncalves, M.D.P.2    Teixeira, J.A.3    Rodrigues, L.G.R.4
  • 6
    • 84863541654 scopus 로고    scopus 로고
    • Monomer and linkage type of galacto-oligosaccharides affect their resistance to ileal digestion and prebiotic properties in rats
    • Hernández-Hernández, O.; Marín-Manzano, M. C.; Rubio, L. A.; Moreno, F. J.; Sanz, M. L.; Clemente, A. Monomer and linkage type of galacto-oligosaccharides affect their resistance to ileal digestion and prebiotic properties in rats J. Nutr. 2012, 142, 1232-1239 10.3945/jn.111.155762
    • (2012) J. Nutr. , vol.142 , pp. 1232-1239
    • Hernández-Hernández, O.1    Marín-Manzano, M.C.2    Rubio, L.A.3    Moreno, F.J.4    Sanz, M.L.5    Clemente, A.6
  • 8
    • 0029137828 scopus 로고
    • The structure and evolution of alpha/beta barrel proteins
    • Reardon, D.; Farber, G. K. The structure and evolution of alpha/beta barrel proteins FASEB J. 1995, 9, 497-503
    • (1995) FASEB J , vol.9 , pp. 497-503
    • Reardon, D.1    Farber, G.K.2
  • 9
    • 34447339120 scopus 로고    scopus 로고
    • In vitro synthesis of artificial polysaccharides by glycosidases and glycosynthases
    • Faijes, M.; Planas, A. In vitro synthesis of artificial polysaccharides by glycosidases and glycosynthases Carbohydr. Res. 2007, 342, 1581-1594 10.1016/j.carres.2007.06.015
    • (2007) Carbohydr. Res. , vol.342 , pp. 1581-1594
    • Faijes, M.1    Planas, A.2
  • 10
    • 70549096101 scopus 로고    scopus 로고
    • Enzymatic transglycosylation for glycoconjugate synthesis
    • Wang, L. X.; Huang, W. Enzymatic transglycosylation for glycoconjugate synthesis Curr. Opin. Chem. Biol. 2009, 13, 592-600 10.1016/j.cbpa.2009.08.014
    • (2009) Curr. Opin. Chem. Biol. , vol.13 , pp. 592-600
    • Wang, L.X.1    Huang, W.2
  • 11
    • 80052742763 scopus 로고    scopus 로고
    • Fructo-oligosaccharide synthesis by mutant versions of Saccharomyces cerevisiae invertase
    • Lafraya, A.; Sanz-Aparicio, J.; Polaina, J.; Marin-Navarro, J. Fructo-oligosaccharide synthesis by mutant versions of Saccharomyces cerevisiae invertase Appl. Environ. Microbiol. 2011, 77, 6148-6157 10.1128/AEM.05032-11
    • (2011) Appl. Environ. Microbiol. , vol.77 , pp. 6148-6157
    • Lafraya, A.1    Sanz-Aparicio, J.2    Polaina, J.3    Marin-Navarro, J.4
  • 12
    • 84925501598 scopus 로고    scopus 로고
    • One-pot production of fructooligosaccharides by a Saccharomyces cerevisiae strain expressing an engineered invertase
    • Marin-Navarro, J.; Talens-Perales, D.; Polaina, J. One-pot production of fructooligosaccharides by a Saccharomyces cerevisiae strain expressing an engineered invertase Appl. Microbiol. Biotechnol. 2015, 99, 2549-2555 10.1007/s00253-014-6312-4
    • (2015) Appl. Microbiol. Biotechnol. , vol.99 , pp. 2549-2555
    • Marin-Navarro, J.1    Talens-Perales, D.2    Polaina, J.3
  • 13
    • 83055180278 scopus 로고    scopus 로고
    • Enzymatic synthesis of galacto-oligosaccharides and other lactose derivatives (hetero-oligosaccharides) from lactose
    • Gänzle, M. G. Enzymatic synthesis of galacto-oligosaccharides and other lactose derivatives (hetero-oligosaccharides) from lactose Int. Dairy J. 2012, 22, 116-122 10.1016/j.idairyj.2011.06.010
    • (2012) Int. Dairy J. , vol.22 , pp. 116-122
    • Gänzle, M.G.1
  • 14
    • 0020570785 scopus 로고
    • Formation of oligosaccharides by β-galactosidase from Streptococcus thermophilus
    • Greenberg, N. A.; Mahoney, R. R. Formation of oligosaccharides by β-galactosidase from Streptococcus thermophilus Food Chem. 1983, 10, 195-204 10.1016/0308-8146(83)90055-9
    • (1983) Food Chem , vol.10 , pp. 195-204
    • Greenberg, N.A.1    Mahoney, R.R.2
  • 15
    • 0033869228 scopus 로고    scopus 로고
    • Transgalactosylation by thermostable beta-glycosidases from Pyrococcus furiosus and Sulfolobus solfataricus. Binding interactions of nucleophiles with the galactosylated enzyme intermediate make major contributions to the formation of new beta-glycosides during lactose conversion
    • Petzelbauer, I.; Reiter, A.; Splechtna, B.; Kosma, P.; Nidetzky, B. Transgalactosylation by thermostable beta-glycosidases from Pyrococcus furiosus and Sulfolobus solfataricus. Binding interactions of nucleophiles with the galactosylated enzyme intermediate make major contributions to the formation of new beta-glycosides during lactose conversion Eur. J. Biochem. 2000, 267, 5055-5066 10.1046/j.1432-1327.2000.01562.x
    • (2000) Eur. J. Biochem. , vol.267 , pp. 5055-5066
    • Petzelbauer, I.1    Reiter, A.2    Splechtna, B.3    Kosma, P.4    Nidetzky, B.5
  • 16
    • 0035260946 scopus 로고    scopus 로고
    • Production of galactooligosaccharides from lactose using a beta-glucosidase from Thermus sp. Z-1
    • Akiyama, K.; Takase, M.; Horikoshi, K.; Okonogi, S. Production of galactooligosaccharides from lactose using a beta-glucosidase from Thermus sp. Z-1 Biosci., Biotechnol., Biochem. 2001, 65, 438-441 10.1271/bbb.65.438
    • (2001) Biosci., Biotechnol., Biochem. , vol.65 , pp. 438-441
    • Akiyama, K.1    Takase, M.2    Horikoshi, K.3    Okonogi, S.4
  • 17
    • 23044490174 scopus 로고    scopus 로고
    • Galacto-oligosaccharide production by a thermostable recombinant β-galactosidase from Thermotoga maritima
    • Ji, E. S.; Park, N. H.; Oh, D. K. Galacto-oligosaccharide production by a thermostable recombinant β-galactosidase from Thermotoga maritima World J. Microbiol. Biotechnol. 2005, 21, 759-764 10.1007/s11274-004-5487-8
    • (2005) World J. Microbiol. Biotechnol. , vol.21 , pp. 759-764
    • Ji, E.S.1    Park, N.H.2    Oh, D.K.3
  • 18
    • 6344268958 scopus 로고    scopus 로고
    • Characterization of a thermostable recombinant beta-galactosidase from Thermotoga maritima
    • Kim, C. S.; Ji, E. S.; Oh, D. K. Characterization of a thermostable recombinant beta-galactosidase from Thermotoga maritima J. Appl. Microbiol. 2004, 97, 1006-1014 10.1111/j.1365-2672.2004.02377.x
    • (2004) J. Appl. Microbiol. , vol.97 , pp. 1006-1014
    • Kim, C.S.1    Ji, E.S.2    Oh, D.K.3
  • 19
    • 84893589483 scopus 로고    scopus 로고
    • Immobilization of thermostable beta-galactosidase on epoxy support and its use for lactose hydrolysis and galactooligosaccharides biosynthesis
    • Marin-Navarro, J.; Talens-Perales, D.; Oude-Vrielink, A.; Canada, F. J.; Polaina, J. Immobilization of thermostable beta-galactosidase on epoxy support and its use for lactose hydrolysis and galactooligosaccharides biosynthesis World J. Microbiol. Biotechnol. 2014, 30, 989-998 10.1007/s11274-013-1517-8
    • (2014) World J. Microbiol. Biotechnol. , vol.30 , pp. 989-998
    • Marin-Navarro, J.1    Talens-Perales, D.2    Oude-Vrielink, A.3    Canada, F.J.4    Polaina, J.5
  • 20
    • 0024357799 scopus 로고
    • A simple method for site-directed mutagenesis using the polymerase chain reaction
    • Hemsley, A.; Arnheim, N.; Toney, M. D.; Cortopassi, G.; Galas, D. J. A simple method for site-directed mutagenesis using the polymerase chain reaction Nucleic Acids Res. 1989, 17, 6545-6551 10.1093/nar/17.16.6545
    • (1989) Nucleic Acids Res , vol.17 , pp. 6545-6551
    • Hemsley, A.1    Arnheim, N.2    Toney, M.D.3    Cortopassi, G.4    Galas, D.J.5
  • 22
    • 39449115394 scopus 로고    scopus 로고
    • I-TASSER server for protein 3D structure prediction
    • Zhang, Y. I-TASSER server for protein 3D structure prediction BMC Bioinf. 2008, 9 (40) 40-49 10.1186/1471-2105-9-40
    • (2008) BMC Bioinf , vol.9 , Issue.40 , pp. 40-49
    • Zhang, Y.1
  • 24
    • 0031015902 scopus 로고    scopus 로고
    • Nomenclature for sugar-binding subsites in glycosyl hydrolases
    • Davies, G. J.; Wilson, K. S.; Henrissat, B. Nomenclature for sugar-binding subsites in glycosyl hydrolases Biochem. J. 1997, 321, 557-559 10.1042/bj3210557
    • (1997) Biochem. J. , vol.321 , pp. 557-559
    • Davies, G.J.1    Wilson, K.S.2    Henrissat, B.3
  • 25
    • 2542505756 scopus 로고    scopus 로고
    • +, and galactosyl C6 hydroxyl and their effects on binding and reactivity of beta-galactosidase
    • +, and galactosyl C6 hydroxyl and their effects on binding and reactivity of beta-galactosidase Biochem. Cell Biol. 2004, 82, 275-284 10.1139/o04-004
    • (2004) Biochem. Cell Biol. , vol.82 , pp. 275-284
    • Xu, J.1    McRae, M.A.2    Harron, S.3    Rob, B.4    Huber, R.E.5
  • 26
    • 0035048410 scopus 로고    scopus 로고
    • His-391 of beta-galactosidase (Escherichia coli) promotes catalyses by strong interactions with the transition state
    • Huber, R. E.; Hlede, I. Y.; Roth, N. J.; McKenzie, K. C.; Ghumman, K. K. His-391 of beta-galactosidase (Escherichia coli) promotes catalyses by strong interactions with the transition state Biochem. Cell Biol. 2001, 79, 183-193 10.1139/o00-101
    • (2001) Biochem. Cell Biol. , vol.79 , pp. 183-193
    • Huber, R.E.1    Hlede, I.Y.2    Roth, N.J.3    McKenzie, K.C.4    Ghumman, K.K.5
  • 27
    • 84860491510 scopus 로고    scopus 로고
    • Substitution for Asn460 cripples beta-galactosidase (Escherichia coli) by increasing substrate affinity and decreasing transition state stability
    • Wheatley, R. W.; Kappelhoff, J. C.; Hahn, J. N.; Dugdale, M. L.; Dutkoski, M. J.; Tamman, S. D.; Fraser, M. E.; Huber, R. E. Substitution for Asn460 cripples beta-galactosidase (Escherichia coli) by increasing substrate affinity and decreasing transition state stability Arch. Biochem. Biophys. 2012, 521, 51-61 10.1016/j.abb.2012.03.014
    • (2012) Arch. Biochem. Biophys. , vol.521 , pp. 51-61
    • Wheatley, R.W.1    Kappelhoff, J.C.2    Hahn, J.N.3    Dugdale, M.L.4    Dutkoski, M.J.5    Tamman, S.D.6    Fraser, M.E.7    Huber, R.E.8
  • 28
    • 17544376273 scopus 로고    scopus 로고
    • The beta-galactosidase (Escherichia coli) reaction is partly facilitated by interactions of His-540 with the C6 hydroxyl of galactose
    • Roth, N. J.; Huber, R. E. The beta-galactosidase (Escherichia coli) reaction is partly facilitated by interactions of His-540 with the C6 hydroxyl of galactose J. Biol. Chem. 1996, 271, 14296-14301 10.1074/jbc.271.24.14296
    • (1996) J. Biol. Chem. , vol.271 , pp. 14296-14301
    • Roth, N.J.1    Huber, R.E.2
  • 30
    • 84870331069 scopus 로고    scopus 로고
    • LacZ beta-galactosidase: Structure and function of an enzyme of historical and molecular biological importance
    • Juers, D. H.; Matthews, B. W.; Huber, R. E. LacZ beta-galactosidase: structure and function of an enzyme of historical and molecular biological importance Protein Sci. 2012, 21, 1792-1807 10.1002/pro.2165
    • (2012) Protein Sci , vol.21 , pp. 1792-1807
    • Juers, D.H.1    Matthews, B.W.2    Huber, R.E.3
  • 31
    • 0345085734 scopus 로고    scopus 로고
    • Modelling and parameter estimation of the enzymatic synthesis of oligosaccharides by beta-galactosidase from Bacillus circulans
    • Boon, M. A.; Janssen, A. E.; van der Padt, A. Modelling and parameter estimation of the enzymatic synthesis of oligosaccharides by beta-galactosidase from Bacillus circulans Biotechnol. Bioeng. 1999, 64, 558-567 10.1002/(SICI)1097-0290(19990905)64:5<558::AID-BIT6>3.0.CO;2-I
    • (1999) Biotechnol. Bioeng. , vol.64 , pp. 558-567
    • Boon, M.A.1    Janssen, A.E.2    Van Der Padt, A.3
  • 32
    • 80051796924 scopus 로고    scopus 로고
    • A pseudo steady-state model for galacto-oligosaccharides synthesis with beta-galactosidase from Aspergillus oryzae
    • Vera, C.; Guerrero, C.; Illanes, A.; Conejeros, R. A pseudo steady-state model for galacto-oligosaccharides synthesis with beta-galactosidase from Aspergillus oryzae Biotechnol. Bioeng. 2011, 108, 2270-2279 10.1002/bit.23201
    • (2011) Biotechnol. Bioeng. , vol.108 , pp. 2270-2279
    • Vera, C.1    Guerrero, C.2    Illanes, A.3    Conejeros, R.4
  • 33
    • 80053452585 scopus 로고    scopus 로고
    • Galacto-oligosaccharides and Other Products Derived from Lactose
    • 3 rd ed. McSweeney, P. L. H. Fox, P. F. Springer: New York
    • Playne, M. J.; Crittenden, R. G. Galacto-oligosaccharides and Other Products Derived from Lactose. In Lactose, Water, Salts and Minor Constituents, 3 rd ed.; McSweeney, P. L. H.; Fox, P. F., Eds.; Springer: New York, 2009; pp 121-201.
    • (2009) Lactose, Water, Salts and Minor Constituents , pp. 121-201
    • Playne, M.J.1    Crittenden, R.G.2
  • 35
    • 40549138422 scopus 로고    scopus 로고
    • Prebiotic evaluation of a novel galactooligosaccharide mixture produced by the enzymatic activity of Bifidobacterium bifidum NCIMB 41171, in healthy humans: A randomized, double-blind, crossover, placebo-controlled intervention study
    • Depeint, F.; Tzortzis, G.; Vulevic, J.; I'Anson, K.; Gibson, G. R. Prebiotic evaluation of a novel galactooligosaccharide mixture produced by the enzymatic activity of Bifidobacterium bifidum NCIMB 41171, in healthy humans: a randomized, double-blind, crossover, placebo-controlled intervention study Am. J. Clin. Nutr. 2008, 87, 785-791
    • (2008) Am. J. Clin. Nutr. , vol.87 , pp. 785-791
    • Depeint, F.1    Tzortzis, G.2    Vulevic, J.3    I'Anson, K.4    Gibson, G.R.5
  • 36
    • 70349245210 scopus 로고    scopus 로고
    • Expression of four beta-galactosidases from Bifidobacterium bifidum NCIMB41171 and their contribution on the hydrolysis and synthesis of galactooligosaccharides
    • Goulas, T.; Goulas, A.; Tzortzis, G.; Gibson, G. R. Expression of four beta-galactosidases from Bifidobacterium bifidum NCIMB41171 and their contribution on the hydrolysis and synthesis of galactooligosaccharides Appl. Microbiol. Biotechnol. 2009, 84, 899-907 10.1007/s00253-009-2009-5
    • (2009) Appl. Microbiol. Biotechnol. , vol.84 , pp. 899-907
    • Goulas, T.1    Goulas, A.2    Tzortzis, G.3    Gibson, G.R.4
  • 37
    • 84893749194 scopus 로고    scopus 로고
    • Kinetic characterization of galacto-oligosaccharide (GOS) synthesis by three commercially important β-galactosidases
    • Warmerdam, A.; Zisopoulos, F. K.; Boom, R. M.; Janssen, A. E. Kinetic characterization of galacto-oligosaccharide (GOS) synthesis by three commercially important β-galactosidases Biotechnol. Prog. 2014, 30, 38-47 10.1002/btpr.1828
    • (2014) Biotechnol. Prog. , vol.30 , pp. 38-47
    • Warmerdam, A.1    Zisopoulos, F.K.2    Boom, R.M.3    Janssen, A.E.4
  • 40
    • 70349411614 scopus 로고    scopus 로고
    • Evolved beta-galactosidases from Geobacillus stearothermophilus with improved transgalactosylation yield for galacto-oligosaccharide production
    • Placier, G.; Watzlawick, H.; Rabiller, C.; Mattes, R. Evolved beta-galactosidases from Geobacillus stearothermophilus with improved transgalactosylation yield for galacto-oligosaccharide production Appl. Environ. Microbiol. 2009, 75, 6312-6321 10.1128/AEM.00714-09
    • (2009) Appl. Environ. Microbiol. , vol.75 , pp. 6312-6321
    • Placier, G.1    Watzlawick, H.2    Rabiller, C.3    Mattes, R.4
  • 41
    • 0035810688 scopus 로고    scopus 로고
    • Improved oligosaccharide synthesis by protein engineering of beta-glucosidase CelB from hyperthermophilic Pyrococcus furiosus
    • Hansson, T.; Kaper, T.; van Der, O. J.; de Vos, W. M.; Adlercreutz, P. Improved oligosaccharide synthesis by protein engineering of beta-glucosidase CelB from hyperthermophilic Pyrococcus furiosus Biotechnol. Bioeng. 2001, 73, 203-210 10.1002/bit.1052
    • (2001) Biotechnol. Bioeng. , vol.73 , pp. 203-210
    • Hansson, T.1    Kaper, T.2    Van Der, O.J.3    De Vos, W.M.4    Adlercreutz, P.5
  • 42
    • 84873722221 scopus 로고    scopus 로고
    • Enhancing the production of galacto-oligosaccharides by mutagenesis of Sulfolobus solfataricus beta-galactosidase
    • Wu, Y.; Yuan, S.; Chen, S.; Wu, D.; Chen, J.; Wu, J. Enhancing the production of galacto-oligosaccharides by mutagenesis of Sulfolobus solfataricus beta-galactosidase Food Chem. 2013, 138, 1588-1595 10.1016/j.foodchem.2012.11.052
    • (2013) Food Chem , vol.138 , pp. 1588-1595
    • Wu, Y.1    Yuan, S.2    Chen, S.3    Wu, D.4    Chen, J.5    Wu, J.6
  • 43
    • 0037452499 scopus 로고    scopus 로고
    • Trp-999 of beta-galactosidase (Escherichia coli) is a key residue for binding, catalysis, and synthesis of allolactose, the natural lac operon inducer
    • Huber, R. E.; Hakda, S.; Cheng, C.; Cupples, C. G.; Edwards, R. A. Trp-999 of beta-galactosidase (Escherichia coli) is a key residue for binding, catalysis, and synthesis of allolactose, the natural lac operon inducer Biochemistry 2003, 42, 1796-1803 10.1021/bi0270642
    • (2003) Biochemistry , vol.42 , pp. 1796-1803
    • Huber, R.E.1    Hakda, S.2    Cheng, C.3    Cupples, C.G.4    Edwards, R.A.5
  • 44
    • 84877143100 scopus 로고    scopus 로고
    • Structural explanation for allolactose (lac operon inducer) synthesis by lacZ beta-galactosidase and the evolutionary relationship between allolactose synthesis and the lac repressor
    • Wheatley, R. W.; Lo, S.; Jancewicz, L. J.; Dugdale, M. L.; Huber, R. E. Structural explanation for allolactose (lac operon inducer) synthesis by lacZ beta-galactosidase and the evolutionary relationship between allolactose synthesis and the lac repressor J. Biol. Chem. 2013, 288, 12993-13005 10.1074/jbc.M113.455436
    • (2013) J. Biol. Chem. , vol.288 , pp. 12993-13005
    • Wheatley, R.W.1    Lo, S.2    Jancewicz, L.J.3    Dugdale, M.L.4    Huber, R.E.5
  • 45
    • 0028178377 scopus 로고
    • Three-dimensional structure of beta-galactosidase from E. coli
    • Jacobson, R. H.; Zhang, X. J.; DuBose, R. F.; Matthews, B. W. Three-dimensional structure of beta-galactosidase from E. coli Nature 1994, 369, 761-766 10.1038/369761a0
    • (1994) Nature , vol.369 , pp. 761-766
    • Jacobson, R.H.1    Zhang, X.J.2    DuBose, R.F.3    Matthews, B.W.4


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