Transgalactosylation by thermostable β-glycosidases from Pyrococcus furiosus and Sulfolobus solfataricus: Binding interactions of nucleophiles with the galactosylated enzyme intermediate make major contributions to the formation of new β-glycosides during lactose conversion

European Journal of Biochemistry

Volumn 267, Issue 16, 2000, Pages 5055-5066

  Petzelbauer, Inge ^a   Reiter, Andreas ^a   Splechtna, Barbara ^a   Kosma, Paul ^a   Nidetzky, Bernd ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

Glycosidase; Oligosaccharides; Pyrococcus; Specificity; Sulfolobus

Indexed keywords

BETA GALACTOSIDASE; GLYCOSIDE; LACTOSE;

ARTICLE; ENZYME MECHANISM; GLUCOSE METABOLISM; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PYROCOCCUS FURIOSUS; SULFOLOBUS SOLFATARICUS;

CARBOHYDRATE SEQUENCE; ENZYME STABILITY; GALACTOSE; GALACTOSYLTRANSFERASES; GLYCOSYLATION; HEAT; KINETICS; MOLECULAR SEQUENCE DATA; OLIGOSACCHARIDES; PYROCOCCUS FURIOSUS; SUBSTRATE SPECIFICITY; SULFOLOBUS; THERMODYNAMICS;

EID: 0033869228     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01562.x     Document Type: Article

References (46)

1. Glycosyl transfer
2. Catalytic mechanisms of enzymic glycosyl transfer
3. Mutagenesis of glycosidases
4. β-Galactosidase
5. A quantitation of the factors which affect the hydrolase and transgalactosylase activities of β-galactosidase (E. coli) on lactose
6. Binding and reactivity at the 'glucose' site of galactosyl-β-galactosidase (Escherichia coli)
7. Crystal structure of the β-glycosidase from the hyperthermophilic archaeon Sulfolobus solfataricus: Resilience as a key factor in thermostability
8. Thermostable β-galactosidase from the archaebacterium Sulfolobus solfataricus. Purification and properties
9. Purification and characterization of an extremely thermostable β-glucosidase from hyperthermophilic archaeon Pyrococcus furiosus
10. An extremely thermostable β-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus; A comparison with other glycosidases
11. Nomenclature for sugar-binding subsites in glycosyl hydrolases
12. Exo-glucosidase activity and substrate specificity of the β-glycosidase isolated from the extreme thermophile Sulfolobus solfataricus
13. Development of an ultra-high-temperature process for the enzymatic hydrolysis of lactose. I. The properties of two thermostable β-glycosidases
14. Preparation of oligosaccharide units library and its utilization
15. Enzymatic synthesis of oligosaccharides
16. Expression and extensive characterization of a β-glycosidase from the extreme thermoacidophilic archaeon Sulfolobus solfataricus in Escherichia coli: Authenticity of the recombinant enzyme
17. Characterization of the celB gene coding for β-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus and its expression and site-directed mutation in Escherichia coli
18. Two-dimensional mapping of N-glycosidically linked asialo-oligosaccharides from glycoproteins as reductively pyridylaminated derivatives using dual separation modes of high-performance capillary electrophoresis
19. Precolumn derivatization for chromatographic and electrophoretic analyses of carbohydrates
20. A capillary electrophoretic study on the specificity of β-galactosidases from Aspergillus oryzae, Escherichia coli, Streptococcus pneumoniae, and Canavalia ensiformis (jack bean)
21. Structure-reactivity relationships for β-galactosidase (Escherichia coli, lac Z). 1. Bronsted parameters for cleavage of alkyl β-D-galactopyranosides
22. Conformational adaptability of the active site of β-galactosidase
23. Partition analysis and the concept of net rate constants as tools in enzyme kinetics
24. Structure-reactivity relationships for β-galactosidase (Escherichia coli, lac Z). 2. Reactions of the galactosyl-enzyme intermediate with alcohols and azide ion
25. Structure-reactivity relationships for β-galactosidase (Escherichia coli, lac Z). 4. Mechanism for reaction of nucleophiles with the galactosyl-enzyme intermediates of E461G and E461Q β-galactosidases
26. 13C NMR spectra of 6'-N-acetylglucosaminyllactose
27. Chemical structure of three neutral trisaccharides isolated in free form from bovine colostrum
28. Formation of oligosaccharides from lactose by Bacillus circulans β-galactosidase
29. Formation of oligosaccharides from lactose by Bacillus circulans β-galactosidase
30. Transferase activity of a β-glycosidase from Thermus thermophilus: Specificities and limits - Application to the synthesis of β-[1→3]-disaccharides
31. Restoration of the activity of active-site mutants of the hyperthermophilic β-glycosidase from Sulfolobus solfataricus: Dependence of the mechanism on the action of external nucleophiles
32. The effect of methanol and dioxan on the β-galactosidase-catalyzed hydrolyses of some β-D-galactopyranosides: Rate-limiting degalactosylation
33. Substrate inhibition and activation kinetics of the β-galactosidase from the extreme thermo-acidophile archaebacterium Caldariella acidophila
34. β-glycosidase from the hyperthermophilic archaeon Sulfolobus solfataricus: Structure and activity in the presence of alcohols
35. The family 1 β-glucosidases from Pyrococcus furiosus and Agrobacterium faecalis share a common catalytic mechanism
36. Nucleophilic competition in some β-galactosidase-catalyzed reactions
37. Ionisation constants of organic acids in aqueous solution
38. The importance of being ribose at the cleavage site in the Tetrahymena ribozyme reaction
39. Binding energy, specificity, and enzymic catalysis: The Circe effect
40. β-Galactosidase of Escherichia coli with substitutions for Glu-461 can be activated by nucleophiles and can form β-galactosyl adducts
41. The enhancement of enzymatic rate accelerations by Bronsted acid-base catalysis
42. Positioning the acid/base catalyst in a glycosidase: Studies with bacillus circulans xylanase
43. Identification of the acid/base catalyst in Agrobacterium faecalis β-glucosidase by kinetic analysis of mutants
44. Identification of two glutamic acid residues essential for catalysis in the β-glycosidase from the thermoacidophilic archaeon Sulfolobus solfataricus
45. Nucleophilic reactivities towards cations