A novel label-free microfluidic paper-based immunosensor for highly sensitive electrochemical detection of carcinoembryonic antigen

Biosensors and Bioelectronics

Volumn 83, Issue , 2016, Pages 319-326

  Wang, Yang ^a,b   Xu, Huiren ^a,b   Luo, Jinping ^a   Liu, Juntao ^a   Wang, Li ^a,b   Fan, Yan ^a,b   Yan, Shi ^c   Yang, Yue ^c   Cai, Xinxia ^a,b  

^a INSTITUTE OF ELECTRONICS   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c PEKING UNIVERSITY CANCER HOSPITAL AND INSTITUTE   (China)

Author keywords

Graphene nanocomposites; Label free; Microfluidic paper based device; Screen printing

Indexed keywords

ANTIGENS; BODY FLUIDS; GRAPHENE; IMMUNOSENSORS; MICROFLUIDICS; NANOCOMPOSITES; PAPER; SCREEN PRINTING; SYNTHESIS (CHEMICAL);

CARCINOEMBRYONIC ANTIGEN; CORRELATION COEFFICIENT; ELECTROCHEMICAL DETECTION; ELECTROCHEMICAL IMMUNOSENSORS; GRAPHENE NANOCOMPOSITES; LABEL FREE; PAPER BASED DEVICES; REFERENCE CONCENTRATION;

CHEMICAL DETECTION;

CARCINOEMBRYONIC ANTIGEN; GOLD NANOPARTICLE; GRAPHENE; NANOCOMPOSITE; THIONINE; GOLD; GRAPHITE; IMMOBILIZED ANTIBODY; METAL NANOPARTICLE;

ANTIGEN ANTIBODY COMPLEX; ANTIGEN DETECTION; ARTICLE; COMPLEX FORMATION; CONCENTRATION (PARAMETERS); ELECTROCHEMICAL ANALYSIS; ELECTRODE; IMMOBILIZATION; IMMUNOASSAY; IMMUNOSENSOR; LIMIT OF DETECTION; MICROFLUIDIC ANALYSIS; PAPER; SCREEN PRINTED WORKING ELECTRODE; SENSITIVITY ANALYSIS; BLOOD; CHEMISTRY; DEVICES; EQUIPMENT DESIGN; HUMAN; POINT OF CARE SYSTEM; ULTRASTRUCTURE;

ANTIBODIES; BIOLOGICAL TESTS; ELECTRICAL PAPERS; ELECTROCHEMISTRY; SENSORS; SILK SCREEN PRINTING;

ANTIBODIES, IMMOBILIZED; CARCINOEMBRYONIC ANTIGEN; ELECTROCHEMICAL TECHNIQUES; ELECTRODES; EQUIPMENT DESIGN; GOLD; GRAPHITE; HUMANS; IMMUNOASSAY; LIMIT OF DETECTION; METAL NANOPARTICLES; MICROFLUIDIC ANALYTICAL TECHNIQUES; NANOCOMPOSITES; PAPER; POINT-OF-CARE SYSTEMS;

EID: 84964497532     PISSN: 09565663     EISSN: 18734235     Source Type: Journal    
DOI: 10.1016/j.bios.2016.04.062     Document Type: Article

References (48)

1. Balandin A.A., Ghosh S., Bao W., Calizo I., Teweldebrhan D., Miao F., Lau C.N. Superior thermal conductivity of single-layer graphene. Nano Lett. 2008, 8:902.
2. Bolotin K.I., Sikes K.J., Jiang Z., Klima M., Fudenberg G., Hone J., Kim P., Stormer H.L. Ultrahigh electron mobility in suspended graphene. Solid State Commun. 2008, 146:351.
3. Cai L.F., Wu Y.Y., Xu C.X., Chen Z.F. A simple paper-based microfluidic device for the determination of the total amino acid content in a tea leaf extract. J. Chem. Educ. 2013, 90(2):232-234.
4. Campanella L., Attioli R., Colapicchioni C., Tomassetti M. New amperometric and potentiometric immunosensors for anti-human immunoglobulin G determinations. Sens. Actuators B 1999, 55:23-32.
5. Chikkaveeraiah B.V., Bhirde A.A., Morgan N.Y., Eden H.S., Chen X. Electrochemical immunosensors for detection of cancer protein biomarkers. ACS Nano 2012, 6(8):6546-6561.
6. Elsharkawy M., Schutzius T.M., Megaridis C.M. Inkjet patterned super hydrophobic paper for open-air surface microfluidic devices. Lab Chip 2014, 14(6):1168-1175.
7. Fenton E.M., Mascarenas M.R., Lpez G.P., Sibbett S.S. Simple and rapid fabrication of paper microfluidic devices utilizing parafilm. Appl. Mater. Interfaces 2009, 1:124-129.
8. Ferrari M. Cancer nanotechnology: opportunities and challenges. Nat. Rev. Cancer 2005, 5:161-171.
9. Grose D., Morrison D.S., Devereux G., Jones R., Sharma D., Selby C., Docherty K., McIntosh D., Nicolson M., McMillan D.C., Milroy R. The impact of comorbidity upon determinants of outcome in patients with lung cancer. Lung Cancer 2015, 87:186-192.
10. 2-G. Appl. Surf. Sci. 2013, 284:862-869.
11. Han J.M., Ma J., Ma Z.F. One-step synthesis of graphene oxide-thionine-Au nanocomposites and its application for electrochemical immunosensing. Biosens. Bioelectron. 2013, 47:243-247.
12. Huang X., Qi X., Boey F., Zhang H. Graphene-based composites. Chem. Soc. Rev. 2012, 41:666.
13. Hawkridge A.M., Muddiman D.C. Mass spectrometry-based biomarker discovery: toward a global proteome index of individuality. Ann. Rev. Anal. Chem. 2009, 2:265-277.
14. Ionescu R.E., Gondran C., Gheber L.A., Cosnier S., Marks R.S. Construction of amperometric immunosensors based on the electrogeneration of a permeable biotinylated polypyrrole film. Anal. Chem. 2004, 76:6808-6813.
15. Jia X.L., Liu Z., Liu N., Ma Z. A label-free immunosensor based on graphene nanocomposites for simultaneous multiplexed electrochemical determination of tumor markers. Biosens. Bioelectron. 2014, 53:160-166.
16. Kleijn S.E., Lai S., Koper M., Unwin P.R. Electrochemistry of nanoparticles. Angew. Chem. Int. Ed. 2014, 53(14):3558-3586.
17. Lee C., Wei X., Kysar J.W., Hone J. Measurement of the elastic properties and intrinsic strength of monolayer graphene. Science 2008, 321:385.
18. Lee S.X., Lim H.N., Ibrahim I., Jamil A., Pandikumar A., Huang N.M. Horseradish peroxidase-labeled silver/reduced graphene oxide thin film-modifi ed screen-printed electrode for detection of carcinoembryonic antigen. Biosens. Bioelectron. 2015.
19. Lefkowitz R.B., Marciniak J.Y., Hu C., Schmid-Schonbein G., Heller M. An electrophoretic method for the detection of chymotrypsin and trypsin activity directly in whole blood. Electrophoresis 2010, 31:403-410.
20. Li D., Mueller M.B., Gilje S., Kaner R.B., Wallace G.G. Processable aqueous dispersions of graphene nanosheets. Nat. Nanotechnol. 2008, 3:101.
21. Li H., Han D., Pauletti G.M., Steckl A.J. Blood coagulation screening using a paper-based microfluidic lateral flow device. Lab Chip 2014, 14(20):4035-4041.
22. Li X., Tian J., Garnier G., Shen W. Fabrication of paper-based microfluidic sensors by printing. Colloids Surf. B: Biointerfaces 2010, 76(2):564-570.
23. Li X., Zhao C., Liu X. A paper-based microfluidic biosensor integrating zinc oxide nanowires for electrochemical glucose detection. Microsyst. Nanoeng. 2015, 1:15014.
24. Liu J.T., Luo J.P., Wang Y., Xu H.R., Wang L., Fan Y., Cai X.X. Application of nanomaterials in electrochemical paper-based biomedical microfluidic devices. Eur. J. Biomed. Res. 2015, 1(3):9-16.
25. Liu Z.F., Jin C.J., Yu Z.L., Zhang J., Liu Y., Zhao H.Y., Jia B., Wang F. Radioimmunotherapy of human colon cancer xenografts with I-131-labeled anti-CEA monoclonal antibody source. Bioconjugate Chem. 2010, 21:314-318.
26. Lu Y., Shi W., Qin J., Lin B. Cheap biomedical devices based on nitrocellulose and wax. Anal. Chem. 2010, 82:329-335.
27. Lv X., Li Y., Cao W., Yan T., Li Y., Du B., Wei Q. A label-free electrochemiluminescence immunosensor based on silver nanoparticle hybridized mesoporous carbon for the detection of Aflatoxin B1. Sens. Actuators B: Chem. 2014, 202(0):53-59.
28. Mao K., Wu D., Li Y., Ma H., Ni Z., Yu H., Luo C., Wei Q., Du B. Label-free electrochemical immunosensor based on graphene/methylene blue nanocomposite. Anal. Biochem. 2012, 422(1):22-27.
29. Martinez A.W., Phillips S.T., Butte M.J., Whitesides G.M. Patterned paper as a platform for inexpensive, low-volume, portable bioassays. Angew. Chem. Int. Ed. 2007, 46:1318-1320.
30. Martinez A.W., Phillips S.T., Wiley B.J., Gupta M., Whitesides G.M. A rapid method for prototyping paper-based microfluidic devices. Lab Chip 2008, 8:2146-2150.
31. Miao J.J., Wang X.B., Lu L.D., Zhu P.Y., Mao C., Zhao H.L., Song Y.C., Shen J. Electrochemical immunosensor based on hyperbranched structure for carcinoembryonic antigen detection. Biosens. Bioelectron. 2014, 58:9-16.
32. Novoselov K.S., Geim A.K., Morozov S.V., Jiang D., Zhang Y., Dubonos S.V., Grigorieva I.V., Firsov A.A. Electric field effect in atomically thin carbon films. Science 2004, 306:666.
33. Ricci F., Adornetto G., Palleschi G. A review of experimental aspects of electrochemical immunosensors. Electrochim. Acta 2012, 84(0):74-83.
34. Rosenfeld T., Bercovici M. 1000-fold sample focusing on paper-based microfluidic devices. Lab Chip 2014, 14(23):4465-4474.
35. Salgado-Figueroa P., Gutiérrez C., Squella J.A. Carbon nanofiber screen printed electrode joined to a flow injection system for nimodipine sensing. Sens. Actuators B: Chem. 2015, 220:456-462.
36. Skladal P. Advances in electrochemical immunosensors. Electroanalysis 1997, 9:737-745.
37. Stoller M.D., Park S., Zhu Y., An J., Ruoff R.S. Graphene-based ultracapacitors. NanoLett 2008, 8:3498.
38. Su B., Tang D., Li Q., Tang J., Chen G. Gold-silver-graphene hybrid nanosheets-based sensors for sensitive amperometric immunoassay of alpha-fetoprotein using nanogold-enclosed titania nanoparticles as labels. Anal. Chim. Acta 2011, 692(1):116-124.
39. Wang J. Electrochemical biosensors: towards point-of-care cancer diagnostics. Biosens. Bioelectron. 2006, 21:1887-1892.
40. Wang R.M., Chen X., Ma J., Ma Z.F. Ultrasensitive detection of carcinoembryonic antigen by a simple label-free immunosensor. Sens. Actuators B: Chem. 2013, 176:1044-1050.
41. Wang R.M., Di J., Ma J., Ma Z.F. Highly sensitive detection of cancer cells by electrochemical impedance spectroscopy. Electrochim. Acta 2012, 61:179-184.
42. Wang Y., Zang D., Ge S., Ge L., Yu J., Yan M. A novel microfluidic origami photo electrochemical sensor based on CdTe quantum dots modified molecularly imprinted polymer and its highly selective detection of S-fenvalerate. Electrochim. Acta 2013, 107:147-154.
43. Wilson M.S. Electrochemical immunosensors for the simultaneous detection of two tumor markers. Anal. Chem. 2005, 77:1496-1502.
44. Wulfkuhle J.D., Liotta L.A., Petricoin E.F. Proteomic applications for the early detection of cancer. Nat. Rev. Cancer 2003, 3:267-275.
45. Xia Y.Y., Si J., Li Z.Y. Fabrication techniques for microfluidic paper-based analytical devices and their applications for biological testing: a review. Biosens. Bioelectron. 2015, 77:774-789.
46. Yan J., Yan M., Ge L., Ge S., Yu J. An origami electrochemiluminescence immunosensor based on gold/graphene for specific, sensitive point-of-care testing of carcinoembryonic antigen. Sens. Actuators B: Chem. 2014, 193:247-254.
47. 4 nanoparticles and thionine mixed graphene sheet. Biosens. Bioelectron. 2013.
48. Yang L., Tang F., Zhang L. A practical glucose biosensor based on Fe3O4nanoparticles and chitosan/nafion composite film. Biosens. Bioelectron. 2009, 25:889-895.