Development of a GIN11/FRT-based multiple-gene integration technique affording inhibitor-tolerant, hemicellulolytic, xylose-utilizing abilities to industrial Saccharomyces cerevisiae strains for ethanol production from undetoxified lignocellulosic hemicelluloses

Microbial Cell Factories

Volumn 13, Issue 1, 2014, Pages

  Hasunuma, Tomohisa ^a   Hori, Yoshimi ^a   Sakamoto, Takatoshi ^a   Ochiai, Misa ^b   Hatanaka, Haruyo ^b   Kondo, Akihiko ^a,c  

^a KOBE UNIVERSITY   (Japan)

^b SUNTORY LTD   (Japan)

^c RIKEN   (Japan)

Author keywords

Bioethanol; Cell surface display; Inhibitor tolerance; Lignocellulose; Maker recycling; Saccharomyces cerevisiae

Indexed keywords

5 HYDROXYMETHYLFURFURAL; ACETIC ACID; ALCOHOL; FORMIC ACID; FURFURAL; GALACTOSE; GLUCOSE; HEMICELLULOSE; LIGNOCELLULOSE; TRANSCRIPTION FACTOR TAL1; VANILLIN; XYLOSE; FUNGAL PROTEIN; LIGNIN; POLYSACCHARIDE;

ADH1 GENE; ARTICLE; ASPERGILLUS ACULEATUS; ASPERGILLUS ORYZAE; BIOMASS FERMENTATION; CONCENTRATION (PARAMETERS); FDH1 GENE; FERMENTATION INHIBITION; FLP RECOMBINASE RECOGNITION TARGET; FUNGAL GENE; FUNGAL GENOME; FUNGAL STRAIN; GENE EXPRESSION; GENETIC PROCEDURES; GIN11 GENE; HYPOCREA JECORINA; NONHUMAN; OPEN READING FRAME; SACCHAROMYCES CEREVISIAE; SCHEFFERSOMYCES STIPITIS; TAL1 GENE; XKS1 GENE; YEAST CELL; BIOSYNTHESIS; GENETICS; HYDROLYSIS; METABOLIC ENGINEERING; METABOLISM;

ETHANOL; FUNGAL PROTEINS; HYDROLYSIS; LIGNIN; METABOLIC ENGINEERING; POLYSACCHARIDES; SACCHAROMYCES CEREVISIAE;

EID: 84964313919     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-014-0145-9     Document Type: Article

References (32)

1. Pauly M, Gille S, Liu L, Mansoori N, de Souza A, Schultink A, Xiong G: Hemicellulose biosynthesis. Planta 2013, 238:627-642.
2. Jeffries TW: Utilization of xylose by bacteria, yeasts, and fungi. Adv Biochem Eng Biotechnol 1983, 27:1-32.
3. Sakamoto T, Hasunuma T, Hori Y, Yamada R, Kondo A: Direct ethanol production from hemicellulosic materials of rice straw by use of an engineered yeast strain codisplaying three types of hemicellulolytic enzymes on the surface of xylose-utilizing Saccharomyces cerevisiae cells. J Biotechnol 2012, 158:203-210.
4. van Zyl WH, Lynd LR, den Haan R, McBride JE: Consolidated bioprocessing for bioethanol production using Saccharomyces cerevisiae. Adv Biochem Eng Biotechnol 2007, 108:205-235.
5. Hahn-Hägerdal B, Karhumaa K, Jeppsson M, Gorwa-Grauslund MF: Metabolic engineering for pentose utilization in Saccharomyces cerevisiae. Adv Biochem Eng Biotechnol 2007, 108:147-177.
6. van Vleet JH, Jeffries TW: Yeast metabolic engineering for hemicellulosic ethanol production. Curr Opin Biotechnol 2009, 20:300-306.
7. Hasunuma T, Kondo A: Development of yeast cell factories for consolidated bioprocessing of lignocellulose to bioethanol through cell surface engineering. Biotechnol Adv 2012, 30:1207-1218.
8. Hasunuma T, Sanda T, Yamada R, Yoshimura K, Ishii J, Kondo A: Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae. Microb Cell Fact 2011, 10:2.
9. Hasunuma T, Sung KM, Sanda T, Yoshimura K, Matsuda F, Kondo A: Efficient fermentation of xylose to ethanol at high formic acid concentrations by metabolically engineered Saccharomyces cerevisiae. Appl Microbiol Biotechnol 2011, 90:997-1004.
10. Sanda T, Hasunuma T, Matsuda F, Kondo A: Repeated-batch fermentation of lignocellulosic hydrolysate to ethanol using a hybrid Saccharomyces cerevisiae strain metabolically engineered for tolerance to acetic and formic acid. Bioresour Technol 2011, 102:7917-7924.
11. Ishii J, Yoshimura K, Hasunuma T, Kondo A: Reduction of furan derivatives by overexpressing NADH-dependent Adh1 improves ethanol fermentation using xylose as sole carbon source with Saccharomyces cerevisiae harboring XR-XDH pathway. Appl Microbiol Biotechnol 2013, 97:2597-2607.
12. Basso LC, de Amorim HV, de Oliveila AJ, Lopes ML: Yeast selection for fuel ethanol production in Brazil. FEMS Yeast Res 2008, 8:1155-1163.
13. Draghici S: Data analysis tools for DNA microarrays. 2nd edition. Boca Raton: CRC Press; 2003.
14. Akada R, Yamamoto J, Yamashita I: Screening and identification of yest sequences cause growth inhibition when overexpressed. Mol Gen Genet 1997, 254:267-274.
15. Storici F, Coglievina M, Bruschi CV: A 2-μm DNA-based marker recycling system for multiple gene disruption in the yeast Saccharomyces cerevisiae. Yeast 1999, 15:271-283.
16. Ismail KSK, Sakamoto T, Hatanaka H, Hasunuma T, Kondo A: Gene expression cross-profiling in genetically advanced industrial Saccharomyces cerevisiae strains during high-temperature ethanol production from xylose. J Biotechnol 2013, 163:50-60.
17. Cantarella M, Cantarella L, Gallifuoco A, Spera A, Alfani F: Comparison of different detoxification methods for steam-exploded poplar wood as a substrate for the bioproduction of ethanol in SHF and SSF. Process Biochem 2004, 39:1533-1542.
18. Zhang J, Zhu Z, Wang X, Wang N, Wang W, Bao J: Biodetoxification of toxins generated from lignocellulose pretreatment using a newly isolated fungus, Amorphotheca resinae ZN1, and the consequent ethanol production. Biotechnol Biofuels 2010, 3:26.
19. Drapcho CM, Nhuan NP, Walker TH: Biofuels Engineering Process Technology. New York: McGraw-Hill; 2008.
20. Kato H, Suyama H, Yamada R, Hasunuma T, Kondo A: Improvement in ethanol production from xylose by mating recombinant xylosefermenting Saccharomyces cerevisiae. Appl Microbiol Biotechnol 2012, 94:1585-1592.
21. Sonderegger M, Jeppsson M, Larsson C, Gorwa-Grauslund MF, Boles E, Olsson L, Spencer-Martins I, Hahn-Hägerdal B, Sauer U: Fermentation performance of engineered and evolved xylose-fermenting Saccharomyces cerevisiae. Biotechnol Bioeng 2004, 87:90-98.
22. Kotaka A, Sahara H, Kondo A, Ueda M, Hata Y: Efficient generation of recessive traits in diploid sake yeast by targeted gene disruption and loss of heterozygosity. Appl Microbiol Biotechnol 2009, 82:387-395.
23. Lynd LR, Laser MS, Bransby D, Dale BE, Davison B, Hamilton R, Himmel M, Keller M, McMillan JD, Sheehan J, Wyman CE: How biotech can transform biofuels. Nat Biotechnol 2008, 26:169-172.
24. Voronovsky AY, Rohulya OV, Abbas CA, Sibirny AA: Development of strains of the thermotolerant yeast Hansenula polymorpha capable of alcoholic fermentation of starch and xylan. Metab Eng 2009, 11:234-242.
25. Fujita Y, Ito J, Ueda M, Fukuda H, Kondo A: Synergistic saccharification, and direct fermentation to ethanol of amorphous cellulose by use of engineered yeast strain codisplaying three types of cellulolytic enzyme. Appl Environ Microbiol 2004, 70:1207-1212.
26. Matano Y, Hasunuma T, Kondo A: Display of cellulases on the cell surface of Saccharomyces cerevisiae for high yield ethanol production from high-solid lignocellulosic biomass. Bioresour Technol 2012, 108:128-133.
27. Mortimer RK, Johnston JR: Genealogy of principal strains of the yeast genetic stock center. Genetics 1986, 113:35-43.
28. Hansen J, Felding T, Johannesen PF, Piskur J, Christensen CL, Olesen K: Further development of the cassette-based pYC plasmid system by incorporation of the dominant hph, nat and AUR1-C gene markers and the lacZ reporter system. FEMS Yeast Res 2003, 4:323-327.
29. Sutcliffe JG: Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proc Natl Acad Sci U S A 1978, 75:3737-3741.
30. Struhl K, Stinchcomb DT, Scherer S, Davis RW: High-frequency transformation of yeast: Autonomous replication of hybrid DNA molecules. Proc Natl Acad Sci U S A 1979, 76:1035-1039.
31. Omura F, Kodama Y, Ashikari T: The basal turnover of yeast branchedchain amino acid permease Bap2p requires its C-terminal tail. FEMS Microbiol Lett 2001, 194:207-214.
32. Fujitomi K, Sanda T, Hasunuma T, Kondo A: Deletion of the PHO13 gene in Saccharomyces cerevisiae improves ethanol production from lignocellulosic hydrolysate in the presence of acetic and formic acids, and furfural. Bioresour Technol 2012, 111:161-166.