CRISPR/Cas9 knockout of HAS2 in rat chondrosarcoma chondrocytes demonstrates the requirement of hyaluronan for aggrecan retention

Matrix Biology

Volumn 56, Issue , 2016, Pages 74-94

  Huang, Yi ^a   Askew, Emily B ^a   Knudson, Cheryl B ^a   Knudson, Warren ^a  

^a EAST CAROLINA UNIVERSITY   (United States)

Author keywords

Aggrecan; Chondrocytes; Hyaluronan; Hyaluronan synthase 2

Indexed keywords

AGGRECAN; CASPASE 9; CELL PROTEIN; CRISPR ASSOCIATED PROTEIN; GUIDE RNA; HYALURONAN SYNTHASE 1; HYALURONAN SYNTHASE 2; HYALURONAN SYNTHASE 3; HYALURONIC ACID BINDING PROTEIN; PROTEOGLYCAN; SYNTHETASE; UNCLASSIFIED DRUG; HAS2 PROTEIN, RAT; HYALURONATE SYNTHETASE; HYALURONIC ACID;

ANIMAL CELL; ARTICLE; ARTICULAR CARTILAGE; BOVINE; CARTILAGE CELL; CELL COUNT; CELL DIVISION; CELL LOSS; CELL MEMBRANE; CELL METABOLISM; CELL PROLIFERATION; CELL SEPARATION; CELL SHAPE; CELL STRUCTURE; CELL SURFACE; CHONDROSARCOMA; CHONDROSARCOMA CHONDROCYTE; COMPARATIVE STUDY; COPY NUMBER VARIATION; DNA SEQUENCE; FLUORESCENCE MICROSCOPY; FOCAL ADHESION; NONHUMAN; PRIORITY JOURNAL; RAT; RNA EDITING; TISSUE ENGINEERING; WILD TYPE; ANIMAL; CELL LINE; CHONDROCYTE; CRISPR CAS SYSTEM; ENZYMOLOGY; GENE KNOCKOUT; GENETICS; METABOLISM; NUCLEOTIDE SEQUENCE;

AGGRECANS; ANIMALS; BASE SEQUENCE; CARTILAGE, ARTICULAR; CELL LINE; CELL PROLIFERATION; CHONDROCYTES; CHONDROSARCOMA; CRISPR-CAS SYSTEMS; GENE KNOCKOUT TECHNIQUES; HYALURONAN SYNTHASES; HYALURONIC ACID; RATS;

EID: 84964304904     PISSN: 0945053X     EISSN: 15691802     Source Type: Journal    
DOI: 10.1016/j.matbio.2016.04.002     Document Type: Article

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