CRISPR/Cas9 mediated generation of stable chondrocyte cell lines with targeted gene knockouts; analysis of an aggrecan knockout cell line

Bone

Volumn 69, Issue , 2014, Pages 118-125

  Yang, Maozhou ^a   Zhang, Liang ^a   Stevens, Jeff ^b   Gibson, Gary ^a  

^a HENRY FORD HOSPITAL   (United States)

^b UNIVERSITY OF IOWA   (United States)

Author keywords

Aggrecan; Chondrocyte; Chondrosarcoma; CRISPR Cas9; RNA guided gene modification

Indexed keywords

AGGRECAN; CARRIER PROTEIN; GLYCOSAMINOGLYCAN; LEUCINE; NUCLEASE; PROTEIN CAS9; PROTEOGLYCAN; SMALL LEUCINE RICH PROTEOGLYCANS; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG; UNTRANSLATED RNA; DEOXYRIBONUCLEASE; TRANSCRIPTOME;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CARCINOGENESIS; CARTILAGE CELL; CHONDROSARCOMA; CONTROLLED STUDY; EXTRACELLULAR MATRIX; GENE EXPRESSION; GENE INACTIVATION; GENE TARGETING; GENETIC TRANSFECTION; GENOME; IMMUNE SYSTEM; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PROTEIN DEPLETION; PROTEIN EXPRESSION; PROTEIN FUNCTION; RAT; RNA EDITING; SARCOMA CELL; SWARM RAT CHONDROSARCOMA CELL LINE; ANIMAL; CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; GENETICS; MOLECULAR GENETICS; NUDE MOUSE; PROCEDURES; SPRAGUE DAWLEY RAT; WESTERN BLOTTING;

AGGRECANS; ANIMALS; BASE SEQUENCE; BLOTTING, WESTERN; CELL LINE; CHONDROCYTES; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; DEOXYRIBONUCLEASES; GENE KNOCKOUT TECHNIQUES; MICE; MICE, NUDE; MOLECULAR SEQUENCE DATA; PHENOTYPE; RATS; RATS, SPRAGUE-DAWLEY; TRANSCRIPTOME; TRANSFECTION;

EID: 84907687928     PISSN: 87563282     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bone.2014.09.005     Document Type: Article

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