Recombinant human deoxyribonuclease therapy improves airway resistance and reduces DNA extracellular traps in a murine acute asthma model

Experimental Lung Research

Volumn 42, Issue 2, 2016, Pages 66-74

  Da Cunha, Aline Andrea ^a   Nuñez, Nailê Karine ^a   De Souza, Rodrigo Godinho ^a   Moraes Vargas, Mauro Henrique ^a   Silveira, Josiane Silva ^a   Antunes, Géssica Luana ^a   Durante, Laíse Da Silva ^a   Porto, Bárbara Nery ^a   Marczak, Elisa Simon ^a   Jones, Marcus Herbert ^a   Pitrez, Paulo Márcio ^a  

^a PONTIFICAL CATHOLIC UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

Author keywords

asthma; DNA extracellular trap; inflammation; lung function; rhDNase

Indexed keywords

ANTIASTHMATIC AGENT; ANTIINFLAMMATORY AGENT; CELL DNA; DEOXYRIBONUCLEASE; EOSINOPHIL PEROXIDASE; OVALBUMIN; RECOMBINANT ENZYME; RECOMBINANT HUMAN DEOXYRIBONUCLEASE; UNCLASSIFIED DRUG; ALLERGEN; DNA; RECOMBINANT PROTEIN;

ADULT; AIRWAY RESISTANCE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIINFLAMMATORY ACTIVITY; APOPTOSIS; ARTICLE; BRONCHOALVEOLAR LAVAGE FLUID; CELL DEATH; CELL HYPERPLASIA; CELL INFILTRATION; CELL VIABILITY; CONTROLLED STUDY; DNA CONTENT; EOSINOPHIL COUNT; EXPERIMENTAL ASTHMA; EXTRACELLULAR TRAP; FEMALE; GOBLET CELL; LEUKOCYTE DIFFERENTIAL COUNT; LUNG FUNCTION; LYMPHOCYTE COUNT; MACROPHAGE; MOUSE; NEUTROPHIL COUNT; NONHUMAN; OVALBUMIN-INDUCED AIRWAY INFLAMMATION; PRIORITY JOURNAL; AIRWAY OBSTRUCTION; ANIMAL; ASTHMA; BAGG ALBINO MOUSE; BRONCHIAL HYPERREACTIVITY; DISEASE MODEL; DRUG EFFECTS; HUMAN; INTRANASAL DRUG ADMINISTRATION; LUNG; METABOLISM; MUCUS; PROCEDURES;

ADMINISTRATION, INTRANASAL; AIRWAY OBSTRUCTION; AIRWAY RESISTANCE; ALLERGENS; ANIMALS; ASTHMA; BRONCHIAL HYPERREACTIVITY; BRONCHOALVEOLAR LAVAGE FLUID; DEOXYRIBONUCLEASES; DISEASE MODELS, ANIMAL; DNA; EXTRACELLULAR TRAPS; FEMALE; HUMANS; LUNG; MICE; MICE, INBRED BALB C; MUCUS; OVALBUMIN; RECOMBINANT PROTEINS;

EID: 84963765047     PISSN: 01902148     EISSN: 15210499     Source Type: Journal    
DOI: 10.3109/01902148.2016.1143537     Document Type: Article

References (28)

1. Lambrecht BN., Hammad H. The immunology of asthma. Nat Immunol. 2014; 16: 45-56
2. Renz H., Bradley K., Saloga J, et al. T cells expressing specific V beta elements regulate immunoglobulin E production and airways responsiveness in vivo. J Exp Med. 1993; 177: 1175-1180
3. Punnonen J., Aversa G., Cocks BG, et al. Role of interleukin-4 and interleukin-13 in synthesis of IgE and expression of CD23 by human B cells. Allergy 1994; 49: 576-586
4. Iwamoto I., Nakajima H., Endo H, et al. Interferon gamma regulates antigen-induced eosinophil recruitment into the mouse airways by inhibiting the infiltration of CD4+ T cells. J Exp Med. 1993; 177: 573-576
5. Global initiative for asthma GINA. (2014) Pocket guide for asthma management and prevention in children. 2014. Available from www.ginasthma.org. Accessed 13 May 2015
6. Woodruff PG., Edmond SD., Singh AK, et al. Sudden onset severe acute asthma: clinical features and response to therapy. Acad Emerg Med. 1998; 5: 695-701
7. Shah PL., Scott SF., Knight RA, et al. In vivo effects of recombinant human DNase I on sputum in patients with cystic fibrosis. Thorax 1996; 51: 119-125
8. Fuchs HJ., Borowitz DS., Christiansen DH, et al. Effect of aerosolized recombinant human DNase on exacerbations of respiratory symptoms and on pulmonary function in patients with cystic fibrosis. The Pulmozyme Study Group. N Engl J Med. 1994; 331: 1423-1424
9. Boogaard R., Smit F., Schornagel R, et al. Recombinant human deoxyribonuclease for the treatment of acute asthma in children. Thorax 2008; 63: 141-146
10. Cunha AA., Porto BN., Nuñez NK, et al. Extracellular DNA traps in bronchoalveolar fluid from a murine eosinophilic pulmonary response. Allergy 2014; 69: 1696-700
11. Hantos Z., Daroczy B., Suki B, et al. Input impedance and peripheral inhomogeneity of dog lungs. J Appl Physiol. 1992; 72: 168-178
12. Zosky GR., Janosi TZ., Adamicza A, et al. The bimodal quasi-static and dynamic elastance of the murine lung. J Appl Physiol. 2008; 105: 685-692
13. Wilson DH., Adams RJ., Tucker G, et al. Trends in asthma prevalence and population changes in South Australia, 1990-2003. Med J Aust. 2006; 184: 226-229
14. Fahy JV., Steiger DJ., Liu J, et al. Markers of mucus secretion and DNA levels in induced sputum from asthmatic and from healthy subjects. Am Rev Respir Dis. 1993; 147: 1132-1137
15. Hogg JC. The pathology of asthma. APMIS 1997; 105: 735-745
16. Kuyper LM., Pare PD., Hogg JC, et al. Characterization of airway plugging in fatal asthma. Am J Med. 2003; 115: 6-11
17. Hodson ME., McKenzie S., Harms HK, et al. Dornase alfa in the treatment of cystic fibrosis in Europe: a report from the epidemiologic registry of cystic fibrosis. Pediatri Pulmonol. 2003; 36: 427-432
18. Paul K., Reichstel E., Ballman M, et al. Effect of treatment with dornase alfa on airway inflammation in patients with cystic fibrosis. Am J Respir Crit Care Med. 2004; 169: 719-725
19. Bakker EM., Volpi S., Salonini E, et al. Improved treatment response to dornase alfa in cystic fibrosis patients using controlled inhalation. Eur Respir J. 2011; 38: 1328-1335
20. Patel A., Harrison E., Durward A, et al. Intratracheal recombinant human deoxyribonuclease in acute life-Threatening asthma refractory to conventional treatment. Br J Anaesth. 2000; 84: 505-507
21. Silverman RA., Foley F., Dalipi R, et al. The use of rhDNAse in severely ill, non-intubated adult asthmatics refractory to bronchodilators: a pilot study. Respir Med. 2012; 106: 1096-1102
22. Konstan MW., Ratjen F. Effect of dornase alfa on inflammation and lung function: potential role in the early treatment of cystic fibrosis. J Cyst Fibros. 2012; 11: 78-83
23. Greally P. Human recombinant DNase for mucus plugging in status asthmaticus. Lancet 1995; 346: 1423-1424
24. Durward A., Forte V., Shemie SD. Resolution of mucus plugging and atelectasis after intratracheal rhDNase therapy in a mechanically ventilated child with refractory status asthmaticus. Crit Care Med. 2000; 28: 560-562
25. Puterman AS., Weinberg EG. rhDNase in acute asthma. Pediatr Pulmonol. 1997; 23: 316-317
26. Kirchner KK., Wagener JS., Khan TZ, et al. Increased DNA levels in bronchoalveolar lavage fluid obtained from infants with cystic fibrosis. Am J Respir Crit Care Med. 1996; 154: 1426-1429
27. Brinkmann V., Reichard U., Goosmann C, et al. Neutrophil extracellular traps kill bacteria. Science 2004; 303: 1532-1535
28. Bates JH. Stochastic model of the pulmonary airway tree and its implications for bronchial responsiveness. J Appl Physiol. 1993; 75: 2493-2499