A high-throughput pipeline for the production of synthetic antibodies for analysis of ribonucleoprotein complexes

RNA

Volumn 22, Issue 4, 2016, Pages 636-655

  Na, Hong ^a   Laver, John D ^a   Jeon, Jouhyun ^a   Singh, Fateh ^a   Ancevicius, Kristin ^b   Fan, Yujie ^a   Cao, Wen Xi ^a   Nie, Kun ^a   Yang, Zhenglin ^a   Luo, Hua ^a   Wang, Miranda ^a,c   Rissland, Olivia ^a,c   Westwood, J Timothy ^b   Kim, Philip M ^a,b   Smibert, Craig A ^a   Lipshitz, Howard D ^a   Sidhu, Sachdev S ^a  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c HOSPITAL FOR SICK CHILDREN RESEARCH INSTITUTE   (Canada)

Author keywords

Fab; Phage display; Ribonucleoprotein complex; RNA binding protein; Synthetic antibody

Indexed keywords

RIBONUCLEOPROTEIN; UNTRANSLATED RNA; ANTIBODY; ANTIGEN; COMPLEMENTARITY DETERMINING REGION; DROSOPHILA PROTEIN; RECOMBINANT PROTEIN;

ANTIBODY PRODUCTION; ANTIGEN EXPRESSION; ANTIGEN PURIFICATION; ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; DROSOPHILA MELANOGASTER; EMBRYO; ESCHERICHIA COLI; HIGH THROUGHPUT SEQUENCING; IMMUNOPRECIPITATION; NONHUMAN; PRIORITY JOURNAL; RNA TRANSCRIPTION; AMINO ACID SEQUENCE; ANIMAL; BIOSYNTHESIS; CHEMISTRY; COMPLEMENTARITY DETERMINING REGION; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES; ANTIGENS; BLOTTING, WESTERN; COMPLEMENTARITY DETERMINING REGIONS; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; IMMUNOPRECIPITATION; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; RIBONUCLEOPROTEINS;

EID: 84962581020     PISSN: 13558382     EISSN: 14699001     Source Type: Journal    
DOI: 10.1261/rna.055186.115     Document Type: Article

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