Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation

Nature Methods

Volumn 12, Issue 8, 2015, Pages 725-731

  Marcon, Edyta ^a   Jain, Harshika ^b   Bhattacharya, Anandi ^c   Guo, Hongbo ^d   Phanse, Sadhna ^e   Pu, Shuye ^f   Byram, Gregory ^g   Collins, Ben C ^h   Dowdell, Evan ^a   Fenner, Maria ⁱ   Guo, Xinghua ^j   Hutchinson, Ashley ^a   Kennedy, Jacob J ^c   Krastins, Bryan ^a   Larsen, Brett ^e   Lin, Zhen Yuan ^a   Lopez, Mary F ^g   Loppnau, Peter ^a   Miersch, Shane ^a   Nguyen, Tin ^b   Olsen, Jonathan B ^a   Paduch, Marcin ^b   Ravichandran, Mani ^b   Seitova, Alma ^d   Vadali, Gouri ^e   Vogelsang, Maryann S ^f   Whiteaker, Jeffrey R ^g   Zhong, Guoqing ^h   Zhong, Nan ^b   Zhao, Lei ^c   Aebersold, Ruedi ^a   Arrowsmith, Cheryl H ^a   Emili, Andrew ^j   Frappier, Lori ^f   Gingras, Anne Claude ^e   Gstaiger, Matthias ^c   Paulovich, Amanda G ^g   Koide, Shohei ^h   Kossiakoff, Anthony A ^a   Sidhu, Sachdev S ^d   Wodak, Shoshana J ^a   Gräslund, Susanne ^b   Greenblatt, Jack F ^d   Edwards, Aled M ^b   more..

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c HOSPITAL FOR SICK CHILDREN   (Canada)

^d Thermo Fisher Scientific   (United States)

^e Institute of Molecular Systems Biology   (Switzerland)

^f UNIVERSITY OF CHICAGO   (United States)

^g Fred Hutchinson Cancer Research Center   (United States)

^h MOUNT SINAI HOSPITAL   (Canada)

ⁱ University of Zurich ^*  (Switzerland)

^j UNIVERSITY OF ZURICH   (Switzerland)

more..

Author keywords

[No Author keywords available]

Indexed keywords

ANTIGEN; PROTEIN; RECOMBINANT ANTIBODY; CHROMATIN; IMMUNOGLOBULIN FRAGMENT; IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY; PEPTIDE LIBRARY; PROTEOME;

ANTIBODY AND IMMUNOGLOBULIN STRUCTURE, FUNCTION AND PRODUCTION; ANTIBODY SELECTIVITY; ANTIBODY SPECIFICITY; ARTICLE; CHROMATIN IMMUNOPRECIPITATION; CONTROLLED STUDY; GOLD STANDARD; HEK293 CELL LINE; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; LIMIT OF QUANTITATION; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN TARGETING; PROTEOMICS; QUANTITATIVE ANALYSIS; WESTERN BLOTTING; BIOLOGY; CHEMISTRY; CHROMATIN; ESCHERICHIA COLI; IMMUNOPRECIPITATION; METABOLISM; MOLECULAR CLONING; PEPTIDE LIBRARY; PROCEDURES; REPRODUCIBILITY;

ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; CHROMATIN; CLONING, MOLECULAR; COMPUTATIONAL BIOLOGY; ESCHERICHIA COLI; HEK293 CELLS; HUMANS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN G; IMMUNOPRECIPITATION; MASS SPECTROMETRY; PEPTIDE LIBRARY; PROTEINS; PROTEOME; PROTEOMICS; REPRODUCIBILITY OF RESULTS;

EID: 84938419721     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth.3472     Document Type: Article

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