Dexamethasone and rosiglitazone are sufficient and necessary for producing functional adipocytes from mesenchymal stem cells

Experimental Biology and Medicine

Volumn 240, Issue 9, 2015, Pages 1235-1246

  Contador, David ^a   Ezquer, Fernando ^a   Espinosa, Maximiliano ^a   Arango Rodriguez, Martha ^a   Puebla, Carlos ^b   Sobrevia, Luis ^b   Conget, Paulette ^a  

^a UNIVERSIDAD DEL DESARROLLO   (Chile)

^b PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE   (Chile)

Author keywords

Adipogenesis; Adipogenic differentiation; Adipokine; Functional adipocyte; Mesechymal stem cell; Multipotent stromal cell

Indexed keywords

2 CHLORO 5 NITROBENZANILIDE; ADIPOCYTOKINE; ADIPONECTIN; CCAAT ENHANCER BINDING PROTEIN; DEXAMETHASONE; GLUCOCORTICOID RECEPTOR; INDOMETACIN; INSULIN; ISOBUTYLMETHYLXANTHINE; LEPTIN; LIPOPROTEIN LIPASE; LONG CHAIN FATTY ACID COENZYME A LIGASE; MIFEPRISTONE; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; PHOSPHOENOLPYRUVATE CARBOXYKINASE (GTP); ROSIGLITAZONE; 2,4 THIAZOLIDINEDIONE DERIVATIVE; 2-CHLORO-5-NITROBENZANILIDE; ANILIDE; MESSENGER RNA;

ADIPOCYTE; ADIPOGENESIS; ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL VIABILITY; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DOG; GENE EXPRESSION; GLUCOSE TRANSPORT; HAMSTER; HUMAN; IMMUNOFLUORESCENCE; INSULIN SENSITIVITY; MESENCHYMAL STEM CELL; MOUSE; NONHUMAN; PROTEIN EXPRESSION; RABBIT; RAT; SIGNAL TRANSDUCTION; ANIMAL; ANTAGONISTS AND INHIBITORS; C57BL MOUSE; CYTOLOGY; DRUG EFFECTS; GENETICS; IN VITRO STUDY; MESENCHYMAL STROMA CELL; MESOCRICETUS; METABOLISM; WISTAR RAT;

ADIPOCYTES; ADIPOGENESIS; ANILIDES; ANIMALS; CCAAT-ENHANCER-BINDING PROTEINS; CELL DIFFERENTIATION; CRICETINAE; DEXAMETHASONE; DOGS; HUMANS; IN VITRO TECHNIQUES; MESENCHYMAL STROMAL CELLS; MESOCRICETUS; MICE; MICE, INBRED C57BL; MIFEPRISTONE; PPAR GAMMA; RABBITS; RATS; RATS, WISTAR; RECEPTORS, GLUCOCORTICOID; RNA, MESSENGER; SIGNAL TRANSDUCTION; THIAZOLIDINEDIONES;

EID: 84960104391     PISSN: 15353702     EISSN: 15353699     Source Type: Journal    
DOI: 10.1177/1535370214566565     Document Type: Article

References (49)

1. Ali AT, Hochfeld WE, Myburgh R, Pepper MS. Adipocyte and adipogenesis. Eur J Cell Biol 2013;92:229–36
2. Rosen ED, MacDougald OA. Adipocyte differentiation from the inside out. Nat Rev Mol Cell Biol 2006;7:885–96
3. Moreno-Navarrete JM and Fernández-Real JM. Adipocyte differentiation chapter 2, In springer scienceþbusiness media LLC. In: Symonds ME (ed.) Adipose Tissue Biology 2012, pp.17–23
4. Poulos SP, Dodson MV, Hausman GJ. Cell line models for differentiation: preadipocytes and adipocytes. Exp Biol Med (Maywood) 2010;235:1185–93
5. Christy RJ, Yang VW, Ntambi JM, Geiman DE, Landshulz WH, Friedman AD, Nakabeppu Y, Kelly TJ, Lane MD. Differentiationinduced gene expression in 3T3-L1 preadipocytes: CCAAT/enhancer binding protein interacts with and activates the promoters of two adipocyte- specific genes. Genes Dev 1989;3:1323–35
6. Cao Z, Umek RM, McKnight SL. Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells. Genes Dev 1991;5:1538–52
7. Tontonoz P, Hu E, Spiegelman BM. Stimulation of adipogenesis in fibroblasts by PPAR gamma 2, a lipid-activated transcription factor (published erratum appears in. Cell 1995;80:1147–56)
8. Yeh WC, Cao Z, Classon M, McKnight SL. Cascade regulation of terminal adipocyte differentiation by three members of the C/EBP family of leucine zipper proteins. Genes Dev 1995;9:168–81
9. Janderova L, McNeil M, Murrell AN, Mynatt RL, Smith SR. Human mesenchymal stem cells as an in vitro model for human adipogenesis. ObesRes 2003;11:65–74
10. Won Park K, Halperin DS, Tontonoz P. Before they were fat: adipocyte progenitors. Cell Metab 2008;8:454–7
11. Rodeheffer MS, Birsoy K, Friedman JM. Identification of white adipocyte progenitor cells in vivo. Cell 2008;135:240–9
12. Pittenger MF. Multilineage potential of adult human mesenchymal stem cells. Science 1999;284:143–7
13. Farmer SR. Transcriptional control of adipocyte formation. Cell Metab 2006;4:263–73
14. Hung SC, Chang CF, Ma HL, Chen TH, Low-Tone Ho L. Gene expression profiles of early adipogenesis in human mesenchymal stem cells. Gene 2004;340:141–50
15. Lee HK, Lee BH, Park SA, Kim CW. The proteomic analysis of an adipocyte differentiated from human mesenchymal stem cells using twodimensional gel electrophoresis. Proteomics 2006;6:1223–29
16. Ylostalo J, Smith JR, Pochampally RR, Matz R, Sekiya I, Larson BL, Vuoristo JT, Prockop DJ. Use of differentiating adult stem cells (marrow stromal cells) to identify new downstream target genes for transcription factors. Stem Cells 2006;24:642–52
17. Jeong JA, Ko KM, Park HS, Lee J, Jang C, Jeon CJ, Koh GY, Kim H. Membrane proteomic analysis of human mesenchymal stromal cells during adipogenesis. Proteomics 2007;7:4181–91
18. Qian SW, Li X, Zhang YY, Huang HY, Liu Y, Sun X, Tang QQ. Characterization of adipocyte differentiation from human mesenchymal stem cells in bone marrow. BMC Dev Biol 2010;10:47
19. Asada M, Rauch A, Shimizu H, Maruyama H, Miyaki S, Shibamori M, Kawasome H, Ishiyama H, Tuckermann J, Asahara H. DNA bindingdependent glucocorticoid receptor activity promotes adipogenesis via Krüppel-like factor 15 gene expression. Lab Invest 2011;91:203–15
20. Ninomiya Y, Sugahara-Yamashita Y, Nakachi Y, Tokuzawa Y, Okazaki Y, Nishiyama M. Development of a rapid culture method to induce adipocyte differentiation of human bone marrow-derived mesenchymal stem cells. Biochem Biophys Res Commun 2010;394:303–8
21. Wang D, Haile A, Jones LC. Rosiglitazone-induced adipogenesis in a bone marrow mesenchymal stem cell line—biomed 2011. Biomed Sci Instrum 2011;47:213–21
22. Conget PA, Minguell JJ. Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells. J Cell Physiol 1999;181:67–73
23. Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop DJ, Horwitz E. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006;8:315–17
24. Lewis-Tuffin LJ, Jewell CM, Bienstock RJ, Collins JB, Cidlowski JA. Human glucocorticoid receptor beta binds RU-486 and is transcriptionally active. Mol Cell Biol 2007;27:2266–82
25. Leesnitzer LM, Parks DJ, Bledsoe RK, Cobb JE, Collins JL, Consler TG, Davis RG, Hull-Ryde EA, Lenhard JM, Patel L, Plunket KD, Shenk JL, Stimmel JB, Therapontos C, Willson TM, Blanchard SG. Functional consequences of cysteine modification in the ligand binding sites of peroxisome proliferator activated receptors by GW9662. Biochemistry 2002;41:6640–50
26. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc 2008;3:1101–8
27. Rojas S, Rojas R, Lamperti L, Casanello P, Sobrevia L. Hyperglycaemia inhibits thymidine incorporation and cell growth via protein kinase C, mitogen-activated protein kinases and nitric oxide in human umbilical vein endothelium. Exp Physiol 2003;88:209–19
28. Prokesch A, Hackl H, Hakim-Weber R, Bornstein SR, Trajanoski Z. Novel insights into adipogenesis from omics data. Curr Med Chem 2009;16:2952–64
29. Lefterova MI, Lazar MA. New developments in adipogenesis. Trends Endocrinol Metab 2009;20:107–14
30. Wiper-Bergeron N, Salem HA, Tomlinson JJ, Wu D, HachéRJG. Glucocorticoid-stimulated preadipocyte differentiation is mediated through acetylation of C/EBPbeta by GCN5. Proc Natl Acad Sci USA 2007;104:2703–8
31. Wiper-Bergeron N, Wu D, Pope L, Schild-Poulter C, HachéRJG. Stimulation of preadipocyte differentiation by steroid through targeting of an HDAC1 complex. EMBO J 2003;22:2135–45
32. Rosen ED, Hsu CH, Wang X, Sakai S, Freeman MW, Gonzalez FJ, Spiegelman BM. C/EBPalpha induces adipogenesis through PPARgamma: a unified pathway. Genes Dev 2002;16:22–6
33. Imai T, Takakuwa R, Marchand S, Dentz E, Bornert JM, Messaddeq N, Wendling O, Mark M, Desvergne B, Wahli W, Chambon P, Metzger D. Peroxisome proliferator-activated receptor gamma is required in mature white and brown adipocytes for their survival in the mouse. Proc Natl Acad Sci USA 2004;101:4543–7
34. Wu Z, Bucher NL, Farmer SR. Induction of peroxisome proliferatoractivated receptor gamma during the conversion of 3T3 fibroblasts into adipocytes is mediated by C/EBPbeta, C/EBPdelta, and glucocorticoids. Mol Cell Biol 1996;16:4128–36
35. Tanaka T, Yoshida N, Kishimoto T, Akira S. Defective adipocyte differentiation in mice lacking the C/EBPbeta and/or C/EBPdelta gene. EMBO J 1997;16:7432–43
36. Moras D, Gronemeyer H. The nuclear receptor ligand-binding domain: structure and function. Curr Opin Cell Biol 1998;10:384–91
37. Hu E, Kim JB, Sarraf P, Spiegelman BM. Inhibition of adipogenesis through MAP kinase-mediated phosphorylation of PPARgamma. Science 1996;274:2100–3
38. Adams M, Reginato MJ, Shao D, Lazar MA, Chatterjee VK. Transcriptional activation by peroxisome proliferator-activated receptor gamma is inhibited by phosphorylation at a consensus mitogen-activated protein kinase site. J Biol Chem 1997;272:5128–32
39. Akiyama TE, Baumann CT, Sakai S, Hager GL, Gonzalez FJ. Selective intranuclear redistribution of PPAR isoforms by RXR alpha. Mol Endocrinol 2002;16:707–21
40. Schmidt SF, Jørgensen M, Chen Y, Nielsen R, Sandelin A, Mandrup S. Cross species comparison of C/EBPa and PPARg profiles in mouse and human adipocytes reveals interdependent retention of binding sites. BMC Genomics 2011;12:152
41. Li X, Ycaza J, Blumberg B. The environmental obesogen tributyltin chloride acts via peroxisome proliferator activated receptor gamma to induce adipogenesis in murine 3T3-L1 preadipocytes. J Steroid Biochem Mol Biol 2011;127:9–15
42. Bogacka I, Xie H, Bray GA, Smith SR. The effect of pioglitazone on peroxisome proliferator-activated receptor-gamma target genes related to lipLabel storage in vivo. Diabetes Care 2004;27:1660–7
43. Haakonsson AK, Stahl Madsen M, Nielsen R, Sandelin A, Mandrup S. Acute genome-wide effects of rosiglitazone on PPARg transcriptional networks in adipocytes. Mol Endocrinol 2013;27:1536–49
44. Garcia de Herreros A, Birnbaum MJ. The regulation by insulin of glucose transporter gene expression in 3T3 adipocytes. J Biol Chem 1989;264:9885–90
45. Mastick CC, Aebersold R, Lienhard GE. Characterization of a major protein in GLUT4 vesicles. Concentration in the vesicles and insulinstimulated translocation to the plasma membrane. J Biol Chem 1994;269:6089–92
46. Thorens B, Mueckler M. Glucose transporters in the 21st Century. Am J Physiol Endocrinol Metab 2010;298:E141–45
47. Cammisotto PG, Bukowiecki LJ. Mechanisms of leptin secretion from white adipocytes. Am J Physiol Cell Physiol 2002;283:C244–50
48. Delporte M-L, Funahashi T, Takahashi M, Matsuzawa Y, Brichard SM. Pre- and post-translational negative effect of beta-adrenoceptor agonists on adiponectin secretion: in vitro and in vivo studies. Biochem J 2002;367:677–85
49. Shigematsu M, Watanabe H, Sugihara H. Proliferation and differentiation of unilocular fat cells in the bone marrow. Cell Struct Funct 1999;24:89–100