Direct CRISPR spacer acquisition from RNA by a natural reverse transcriptase-Cas1 fusion protein

Science

Volumn 351, Issue 6276, 2016, Pages

  Silas, Sukrit ^a,b   Mohr, Georg ^c   Sidote, David J ^c   Markham, Laura M ^c   Sanchez Amat, Antonio ^d   Bhaya, Devaki ^e   Lambowitz, Alan M ^c   Fire, Andrew Z ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b STANFORD UNIVERSITY   (United States)

^c UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^d UNIVERSITY OF MURCIA   (Spain)

^e GEOPHYSICAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CAS1 PROTEIN; CAS2 PROTEIN; HYBRID PROTEIN; RNA; RNA DIRECTED DNA POLYMERASE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CHIMERIC PROTEIN; CRISPR ASSOCIATED PROTEIN; DNA;

ADAPTATION; BACTERIUM; DNA; GENE EXPRESSION; IMMUNE SYSTEM; PROTEIN; RNA;

ARTICLE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; IN VITRO STUDY; IN VIVO STUDY; MARINE BACTERIUM; MARINOMONAS; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION; CLASSIFICATION; CRISPR CAS SYSTEM; ENZYMOLOGY; GENETICS; INTRON; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYLOGENY; PROTEIN TERTIARY STRUCTURE; RNA SPLICING;

BACTERIA (MICROORGANISMS); MARINOMONAS MEDITERRANEA; PROKARYOTA;

BACTERIAL PROTEINS; BASE SEQUENCE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-ASSOCIATED PROTEINS; CRISPR-CAS SYSTEMS; DNA; INTRONS; MARINOMONAS; MOLECULAR SEQUENCE DATA; MUTANT CHIMERIC PROTEINS; PHYLOGENY; PROTEIN STRUCTURE, TERTIARY; RNA; RNA SPLICING; RNA-DIRECTED DNA POLYMERASE;

EID: 84959419241     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.aad4234     Document Type: Article

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