Culture-independent method for identification of microbial enzyme-encoding genes by activity-based single-cell sequencing using a water-in-oil microdroplet platform

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Nakamura, Kazuki ^a   Iizuka, Ryo ^a   Nishi, Shinro ^b   Yoshida, Takao ^b   Hatada, Yuji ^b   Takaki, Yoshihiro ^b   Iguchi, Ayaka ^c   Yoon, Dong Hyun ^c   Sekiguchi, Tetsushi ^c   Shoji, Shuichi ^c   Funatsu, Takashi ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b JAPAN AGENCY FOR MARINE EARTH SCIENCE AND TECHNOLOGY   (Japan)

^c WASEDA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; BETA GLUCOSIDASE; FLUORESCENT DYE; OIL; RNA 16S; SEA WATER; WATER;

BACTERIUM; CHEMISTRY; CLASSIFICATION; CYTOLOGY; DEVICES; DNA SEQUENCE; FLUORESCENCE MICROSCOPY; GENETICS; KINETICS; METABOLISM; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; PROCEDURES; REPRODUCIBILITY; SINGLE CELL ANALYSIS; STANDARDS;

BACTERIA; BACTERIAL PROTEINS; BACTERIOLOGICAL TECHNIQUES; BETA-GLUCOSIDASE; FLUORESCENT DYES; KINETICS; MICROSCOPY, FLUORESCENCE; OILS; REPRODUCIBILITY OF RESULTS; RNA, RIBOSOMAL, 16S; SEAWATER; SEQUENCE ANALYSIS, DNA; SINGLE-CELL ANALYSIS; WATER; WATER MICROBIOLOGY;

EID: 84959387605     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep22259     Document Type: Article

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