Transient expression in HEK 293 cells: An alternative to E. coli for the production of secreted and intracellular mammalian proteins

Methods in Molecular Biology

Volumn 1258, Issue , 2015, Pages 209-222

  Nettleship, Joanne E ^a,b   Watson, Peter J ^c   Rahman Huq, Nahid ^a,b   Fairall, Louise ^c   Posner, Mareike G ^d   Upadhyay, Abhishek ^d   Reddivari, Yamini ^a,b   Chamberlain, Jonathan M G ^e   Kolstoe, Simon E ^e   Bagby, Stefan ^d   Schwabe, John W R ^c   Owens, Raymond J ^a,b  

^a RUTHERFORD APPLETON LABORATORY   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c UNIVERSITY OF LEICESTER   (United Kingdom)

^d University of Bath   (United Kingdom)

^e UNIVERSITY OF PORTSMOUTH   (United Kingdom)

Author keywords

FLAG purification; HEK; Intracellular protein; Mammalian; Protein complexes; Secreted protein; Transient transfection

Indexed keywords

AMYLOID P COMPONENT; CELL PROTEIN; HISTONE DEACETYLASE 3; PROTEIN KINASE; PROTEIN KINASE C; PROTEIN KINASE M ZETA; RECOMBINANT PROTEIN; SECRETED PROTEIN; SILENCING MEDIATOR OF RETINOID AND THYROID HORMONE RECEPTOR; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL MANIPULATION; COMPLEX FORMATION; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; HEK293 CELL LINE; HUMAN; MAMMAL CELL; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BLOOD LEVEL; PROTEIN GLYCOSYLATION; PROTEIN MODIFICATION; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN SECRETION; TRANSCRIPTION REGULATION; TRANSIENT EXPRESSION; TRANSIENT TRANSFECTION; ANIMAL; GENETIC TRANSFECTION; GENETICS; MAMMAL; METABOLISM; PROCEDURES;

MAMMALIA;

ANIMALS; ESCHERICHIA COLI; HEK293 CELLS; HUMANS; MAMMALS; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 84958666963     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2205-5_11     Document Type: Article

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