PTEN modulates EGFR late endocytic trafficking and degradation by dephosphorylating Rab7

Nature Communications

Volumn 7, Issue , 2016, Pages

  Shinde, Swapnil Rohidas ^a,b   Maddika, Subbareddy ^a  

^a CENTRE FOR DNA FINGERPRINTING AND DIAGNOSTICS   (India)

^b MANIPAL UNIVERSITY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR; GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR; PHOSPHATIDYLINOSITOL 3,4,5 TRISPHOSPHATE 3 PHOSPHATASE; PHOSPHOPROTEIN PHOSPHATASE; RAB7 PROTEIN; EGFR PROTEIN, HUMAN; EPIDERMAL GROWTH FACTOR; PHOSPHOPROTEIN; PTEN PROTEIN, HUMAN; RAB PROTEIN;

BIODEGRADATION; MATURATION; MUSCLE; PHOSPHATASE; PROTEIN; TUMOR;

ARTICLE; CELL MATURATION; CELL MIGRATION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ENDOCYTOSIS; ENDOSOME; ENZYME ACTIVITY; HUMAN; HUMAN CELL; PROTEIN DEGRADATION; PROTEIN DEPHOSPHORYLATION; PROTEIN INTERACTION; SIGNAL TRANSDUCTION; FLUORESCENT ANTIBODY TECHNIQUE; HEK293 CELL LINE; HELA CELL LINE; IMMUNOPRECIPITATION; METABOLISM; PROTEIN TRANSPORT; TUMOR CELL LINE; WESTERN BLOTTING;

BLOTTING, WESTERN; CELL LINE, TUMOR; ENDOCYTOSIS; ENDOSOMES; EPIDERMAL GROWTH FACTOR; FLUORESCENT ANTIBODY TECHNIQUE; HEK293 CELLS; HELA CELLS; HUMANS; IMMUNOPRECIPITATION; PHOSPHOPROTEINS; PROTEIN TRANSPORT; PTEN PHOSPHOHYDROLASE; RAB GTP-BINDING PROTEINS; RECEPTOR, EPIDERMAL GROWTH FACTOR; SIGNAL TRANSDUCTION;

EID: 84957991211     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms10689     Document Type: Article

References (39)

1. Di Cristofano, A. & Pandolfi, P. P. The multiple roles of PTEN in tumor suppression. Cell 100, 387-390 (2000).
2. Li, J. et al. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer. Science 275, 1943-1947 (1997).
3. Myers, M. P. et al. P-TEN, the tumor suppressor from human chromosome 10q23, is a dual-specificity phosphatase. Proc. Natl Acad. Sci. USA 94, 9052-9057 (1997).
4. Ali, I. U., Schriml, L. M. & Dean, M. Mutational spectra of PTEN/MMAC1 gene: a tumor suppressor with lipid phosphatase activity. J. Natl Cancer Inst. 91, 1922-1932 (1999).
5. Eng, C. PTEN: one gene, many syndromes. Hum. Mutat. 22, 183-198 (2003).
6. Maehama, T. & Dixon, J. E. The tumor suppressor, PTEN/MMAC1, dephosphorylates the lipid second messenger, phosphatidylinositol 3,4,5-trisphosphate. J. Biol. Chem. 273, 13375-13378 (1998).
7. Zhang, X. C., Piccini, A., Myers, M. P., Van Aelst, L. & Tonks, N. K. Functional analysis of the protein phosphatase activity of PTEN. Biochem. J. 444, 457-464 (2012).
8. Myers, M. P. et al. The lipid phosphatase activity of PTEN is critical for its tumor supressor function. Proc. Natl Acad. Sci. USA 95, 13513-13518 (1998).
9. Dey, N. et al. The protein phosphatase activity of PTEN regulates SRC family kinases and controls glioma migration. Cancer Res. 68, 1862-1871 (2008).
10. Stambolic, V. et al. Negative regulation of PKB/Akt-dependent cell survival by the tumor suppressor PTEN. Cell 95, 29-39 (1998).
11. Tomas, A., Futter, C. E. & Eden, E. R. EGF receptor trafficking: consequences for signaling and cancer. Trends Cell Biol. 24, 26-34 (2014).
12. Vieira, A. V., Lamaze, C. & Schmid, S. L. Control of EGF receptor signaling by clathrin-mediated endocytosis. Science 274, 2086-2089 (1996).
13. Goh, L. K., Huang, F., Kim, W., Gygi, S. & Sorkin, A. Multiple mechanisms collectively regulate clathrin-mediated endocytosis of the epidermal growth factor receptor. J. Cell Biol. 189, 871-883 (2010).
14. Brankatschk, B. et al. Regulation of the EGF transcriptional response by endocytic sorting. Sci. Signal. 5, ra21 (2012).
15. Futter, C. E., Pearse, A., Hewlett, L. J. & Hopkins, C. R. Multivesicular endosomes containing internalized EGF-EGF receptor complexes mature and then fuse directly with lysosomes. J. Cell Biol. 132, 1011-1023 (1996).
16. Naguib, A. et al. PTEN functions by recruitment to cytoplasmic vesicles. Mol. Cell 58, 255-268 (2015).
17. Numrich, J. & Ungermann, C. Endocytic Rabs in membrane trafficking and signaling. Biol. Chem. 395, 327-333 (2014).
18. Spinosa, M. R. et al. Functional characterization of Rab7 mutant proteins associated with Charcot-Marie-Tooth type 2B disease. J. Neurosci. 28, 1640-1648 (2008).
19. Ceresa, B. P. & Bahr, S. J. rab7 activity affects epidermal growth factor:epidermal growth factor receptor degradation by regulating endocytic trafficking from the late endosome. J. Biol. Chem. 281, 1099-1106 (2006).
20. Vanlandingham, P. A. & Ceresa, B. P. Rab7 regulates late endocytic trafficking downstream of multivesicular body biogenesis and cargo sequestration. J. Biol. Chem. 284, 12110-12124 (2009).
21. Bucci, C., Thomsen, P., Nicoziani, P., McCarthy, J. & van Deurs, B. Rab7: a key to lysosome biogenesis. Mol. Biol. Cell 11, 467-480 (2000).
22. Vitelli, R. et al. Role of the small GTPase Rab7 in the late endocytic pathway. J. Biol. Chem. 272, 4391-4397 (1997).
23. Zhou, H. et al. Toward a comprehensive characterization of a human cancer cell phosphoproteome. J. Proteome Res. 12, 260-271 (2013).
24. Sharma, K. et al. Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep. 8, 1583-1594 (2014).
25. Huotari, J. et al. Cullin-3 regulates late endosome maturation. Proc. Natl Acad. Sci. USA 109, 823-828 (2012).
26. Pfeffer, S. A model for Rab GTPase localization. Biochem. Soc. Trans. 33, 627-630 (2005).
27. Nordmann, M. et al. The Mon1-Ccz1 complex is the GEF of the late endosomal Rab7 homolog Ypt7. Curr. Biol. 20, 1654-1659 (2010).
28. Kinchen, J. M. & Ravichandran, K. S. Identification of two evolutionarily conserved genes regulating processing of engulfed apoptotic cells. Nature 464, 778-782 (2010).
29. Li, F. et al. The role of the hypervariable C-terminal domain in Rab GTPases membrane targeting. Proc. Natl Acad. Sci. USA 111, 2572-2577 (2014).
30. Tibarewal, P. et al. PTEN protein phosphatase activity correlates with control of gene expression and invasion, a tumor-suppressing phenotype, but not with AKT activity. Sci. Signal. 5, ra18 (2012).
31. Leslie, N. R., Yang, X., Downes, C. P. & Weijer, C. J. PtdIns(3,4,5)P(3)-dependent and -independent roles for PTEN in the control of cell migration. Curr. Biol. 17, 115-125 (2007).
32. Mahimainathan, L. & Choudhury, G. G. Inactivation of platelet-derived growth factor receptor by the tumor suppressor PTEN provides a novel mechanism of action of the phosphatase. J. Biol. Chem. 279, 15258-15268 (2004).
33. Ning, K. et al. Dual neuroprotective signaling mediated by downregulating two distinct phosphatase activities of PTEN. J. Neurosci. 24, 4052-4060 (2004).
34. Maier, D. et al. The PTEN lipid phosphatase domain is not required to inhibit invasion of glioma cells. Cancer Res. 59, 5479-5482 (1999).
35. Freeman, D. J. et al. PTEN tumor suppressor regulates p53 protein levels and activity through phosphatase-dependent and -independent mechanisms. Cancer Cell 3, 117-130 (2003).
36. Rak, A. et al. Structure of the Rab7:REP-1 complex: insights into the mechanism of Rab prenylation and choroideremia disease. Cell 117, 749-760 (2004).
37. Maddika, S. & Chen, J. Protein kinase DYRK2 is a scaffold that facilitates assembly of an E3 ligase. Nat. Cell Biol. 11, 409-419 (2009).
38. Maddika, S. et al. WWP2 is an E3 ubiquitin ligase for PTEN. Nat. Cell Biol. 13, 728-733 (2011).
39. Stenmark, H. et al. Inhibition of rab5 GTPase activity stimulates membrane fusion in endocytosis. EMBO J. 13, 1287-1296 (1994).