Gut microbial dysbiosis may predict diarrhea and fatigue in patients undergoing pelvic cancer radiotherapy: A pilot study

PLoS ONE

Volumn 10, Issue 5, 2015, Pages

  Wang, Aiping ^a   Ling, Zongxin ^b   Yang, Zhixiang ^c   Kiela, Pawel R ^d   Wang, Tao ^a   Wang, Cheng ^a   Cao, Le ^a   Geng, Fang ^a   Shen, Mingqiang ^a   Ran, Xinze ^a   Su, Yongping ^a   Cheng, Tianmin ^a   Wang, Junping ^a  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

^b ZHEJIANG UNIVERSITY   (China)

^c ZHONGSHAN HOSPITAL OF FUDAN UNIVERSITY   (China)

^d UNIVERSITY OF ARIZONA COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA 1 ANTITRYPSIN; CITRULLINE; HAPTOGLOBIN; OROSOMUCOID; RNA 16S; TUMOR NECROSIS FACTOR ALPHA; BACTERIAL RNA;

ADULT; ALISTIPES; ARTICLE; BACTERIUM; BACTEROIDES; BACTEROIDETES; CANCER RADIOTHERAPY; CLINICAL EFFECTIVENESS; CLOSTRIDIUM; COMMUNITY STRUCTURE; CONTROLLED STUDY; DIARRHEA; DISEASE SEVERITY; DYSBIOSIS; ERYSIPELOTRICHACEAE; ESCHERICHIA; FAECALIBACTERIUM; FATIGUE; FEMALE; FIRMICUTES; HUMAN; INTESTINE CELL; INTESTINE FLORA; LACHNOSPIRACEA; LACHNOSPIRACEAE; MAJOR CLINICAL STUDY; MALE; MEGAMONAS; MICROBIAL DIVERSITY; OSCILLIBACTER; PELVIS CANCER; PILOT STUDY; PROTEIN BLOOD LEVEL; PYROSEQUENCING; QUESTIONNAIRE; RADIATION RESPONSE; ROSEBURIA; SPECIES RICHNESS; STREPTOCOCCUS; TREATMENT DURATION; TREATMENT RESPONSE; ADVERSE EFFECTS; COMPLICATION; GASTROINTESTINAL TRACT; MICROBIOLOGY; MICROFLORA; MIDDLE AGED; PELVIC NEOPLASMS; PHYSIOLOGY;

ADULT; DIARRHEA; FATIGUE; FEMALE; GASTROINTESTINAL TRACT; HUMANS; MALE; MICROBIOTA; MIDDLE AGED; PELVIC NEOPLASMS; PILOT PROJECTS; RADIOTHERAPY; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S;

EID: 84957585563     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0126312     Document Type: Article

References (34)

1. Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin. 2011; 61: 69-90. doi:10.3322/caac.20107 PMID: 21296855.
2. Haddock MG, Sloan JA, Bollinger JW, Soori G, Steen PD, Martenson JA. Patient assessment of bowel function during and after pelvic radiotherapy: Results of a prospective phase III North Central Cancer Treatment Group clinical trial. J Clin Oncol. 2007; 25: 1255-1259. PMID: 17401014.
3. Hogan NM, Kerin MJ, Joyce MR. Gastrointestinal complications of pelvic radiotherapy: medical and surgical management strategies. Curr Probl Surg. 2013; 50: 395-407. doi:10.1067/j.cpsurg.2013.04.004 PMID: 23930906.
4. Andreyev HJ, Wotherspoon A, Denham JW, Hauer-Jensen M. Defining pelvic-radiation disease for the survivorship era. Lancet Oncol. 2010; 11: 310-312. doi:10.1016/S1470-2045(10)70026-7 PMID: 20149738.
5. Ahlberg K, Ekman T, Gaston-Johansson F. The experience of fatigue, other symptoms and global quality of life during radiotherapy for uterine cancer. Int J Nurs Stud. 2005; 42: 377-386. PMID: 15847900.
6. Hauer-Jensen M, Denham JW, Andreyev HJ. Radiation enteropathy-pathogenesis, treatment and prevention. Nat Rev Gastroenterol Hepatol. 2014; 11: 470-479. doi:10.1038/nrgastro.2014.46 PMID: 24686268.
7. Abayomi J, Kirwan J, Hackett A, Bagnall G. A study to investigate women's experiences of radiation enteritis following radiotherapy for cervical cancer. J Hum Nutr Diet. 2005; 18: 353-363. PMID: 16150131.
8. Jakobsson S, Ahlberg K, Taft C, Ekman T. Exploring a link between fatigue and intestinal injury during pelvic radiotherapy. Oncologist. 2010; 15: 1009-1015. doi:10.1634/theoncologist.2010-0097 PMID: 20667967.
9. Ryan JL, Carroll JK, Ryan EP, Mustian KM, Fiscella K, Morrow GR. Mechanisms of cancer-related fatigue. Oncologist 12 Suppl. 2007; 1: 22-34.
10. Mayer EA, Knight R, Mazmanian SK, Cryan JF, Tillisch K. Gut microbes and the brain: paradigm shift in neuroscience. J Neurosci. 2014; 34: 15490-15496doi:10.1523/JNEUROSCI.3299-14.2014 PMID: 25392516.
11. Manichanh C, Varela E, Martinez C, Antolin M, Llopis M, Dore J, et al. The gut microbiota predispose to the pathophysiology of acute postradiotherapy diarrhea. Am J Gastroenterol. 2008; 103: 1754-1761. doi:10.1111/j.1572-0241.2008.01868.x PMID: 18564125.
12. Kim YS, Kim J, Park SJ. High-throughput 16S rRNA gene sequencing reveals alterations of mouse intestinal microbiota after radiotherapy. Anaerobe. 2015; 33C: d.
13. Crawford PA, Gordon JI. Microbial regulation of intestinal radiosensitivity. Proc Natl Acad Sci U S A. 2005; 102: 13254-13259. PMID: 16129828.
14. Delia P, Sansotta G, Donato V, Messina G, Frosina P, Pergolizzi S, et al. Prevention of radiationinduced diarrhea with the use of VSL#3, a new high-potency probiotic preparation. Am J Gastroenterol. 2002; 97: 2150-2152. PMID: 12190202.
15. Urbancsek H, Kazar T, Mezes I, Neumann K. Results of a double-blind, randomized study to evaluate the efficacy and safety of Antibiophilus in patients with radiation-induced diarrhoea. Eur J Gastroenterol Hepatol. 2001; 13: 391-396. PMID: 11338068.
16. Ferreira MR, Muls A, Dearnaley DP, Andreyev HJ. Microbiota and radiation-induced bowel toxicity: lessons from inflammatory bowel disease for the radiation oncologist. Lancet Oncol. 2014; 15: E139-e147. doi:10.1016/S1470-2045(13)70504-7 PMID: 24599929.
17. Schubert C, Hong S, Natarajan L, Mills PJ, Dimsdale JE. The association between fatigue and inflammatory marker levels in cancer patients: A quantitative review. Brain Behav Immun. 2007; 21: 413-427. PMID: 17178209.
18. Trotti A, Byhardt R, Stetz J, Gwede C, Corn B, Fu K, et al. Common toxicity criteria: version 2.0. an improved reference for grading the acute effects of cancer treatment: impact on radiotherapy. Int J Radiat Oncol Biol Phys. 2000; 47: 13-47. PMID: 10758303.
19. Hagelin CL, Wengstrom Y, Runesdotter S, Furst CJ. The psychometric properties of the Swedish Multidimensional Fatigue Inventory MFI-20 in four different populations. Acta Oncol. 2007; 46: 97-104. PMID: 17438711.
20. Ling Z, Liu X, Luo Y, Yuan L, Nelson KE, Wang Y, et al. Pyrosequencing analysis of the human microbiota of healthy Chinese undergraduates. BMC Genomics. 2013; 14: 390doi:10.1186/1471-2164-14-390 PMID: 23758874.
21. Fierer N, Hamady M, Lauber CL, Knight R. The influence of sex, handedness, and washing on the diversity of hand surface bacteria. Proc Natl Acad Sci U S A. 2008; 105: 17994-17999. doi:10.1073/pnas.0807920105 PMID: 19004758.
22. Haas BJ, Gevers D, Earl AM, Feldgarden M, Ward DV, et al. (2011) Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons. Genome Res. 2011; 21: 494-504. doi:10.1101/gr.112730.110 PMID: 21212162.
23. Pruesse E, Quast C, Knittel K, Fuchs BM, Ludwig W, Peplies J, et al. SILVA: A comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Res. 2007; 35: 7188-7196. PMID: 17947321.
24. Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, et al. Introducing mothur: opensource, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol. 2009; 75: 7537-7541. doi:10.1128/AEM.01541-09 PMID: 19801464.
25. Rosen GL, Reichenberger ER, Rosenfeld AM. NBC: The Naive Bayes Classification tool webserver for taxonomic classification of metagenomic reads. Bioinformatics. 2011; 27: 127-129. doi:10.1093/bioinformatics/btq619PMID: 21062764.
26. Lozupone C, Hamady M, Knight R. UniFrac-an online tool for comparing microbial community diversity in a phylogenetic context. BMC Bioinformatics. 2006; 7: 371. PMID: 16893466.
27. Crenn P, Coudray-Lucas C, Thuillier F, Cynober L, Messing B. Postabsorptive plasma citrulline concentration is a marker of absorptive enterocyte mass and intestinal failure in humans. Gastroenterology. 2000; 119: 1496-1505. PMID: 11113071.
28. Lutgens LC, Deutz N, Granzier-Peeters M, Beets-Tan R, De Ruysscher D, Gueulette J, et al. Plasma citrulline concentration: A surrogate end point for radiation-induced mucosal atrophy of the small bowel. A feasibility study in 23 patients. Int J Radiat Oncol Biol Phys. 2004; 60: 275-285. PMID: 15337566.
29. Atherton PJ, Halyard MY, Sloan JA, Miller RC, Deming RL, Tai TH, et al. Assessment of patientreported measures of bowel function before and after pelvic radiotherapy: An ancillary study of the North Central Cancer Treatment Group study N00CA. Support Care Cancer. 2013; 21: 1193-1199. doi:10.1007/s00520-012-1648-8 PMID: 23151649.
30. Wedlake L, McGough C, Hackett C, Thomas K, Blake P, Harrington K, et al. Can biological markers act as non-invasive, sensitive indicators of radiation-induced effects in the gastrointestinal mucosa? Aliment Pharmacol Ther. 2008; 27: 980-987. doi:10.1111/j.1365-2036.2008.03663.x PMID: 18315578.
31. Touchefeu Y, Montassier E, Nieman K, Gastinne T, Potel G, Bruley des Varannes S, et al. Systematic review: The role of the gut microbiota in chemotherapy- or radiation-induced gastrointestinal mucositis -current evidence and potential clinical applications. Aliment Pharmacol Ther. 2014; 40: 409-421. doi:10.1111/apt.12878PMID: 25040088.
32. Atarashi K, Tanoue T, Oshima K, Suda W, Nagano Y, Nishikawa H, et al. Treg induction by a rationally selected mixture of Clostridia strains from the human microbiota. Nature. 2013; 500: 232-236. doi:10.1038/nature12331 PMID: 23842501.
33. Miquel S, Martin R, Rossi O, Bermudez-Humaran LG, Chatel JM, Sokol H, et al. Faecalibacterium prausnitzii and human intestinal health. Curr Opin Microbiol. 2013; 16: 255-261. doi:10.1016/j.mib.2013.06.003 PMID: 23831042.
34. Fuccio L, Frazzoni L, Guido A. Prevention of pelvic radiation disease. World J Gastrointest Pharmacol Ther. 2015; 6: 1-9. doi:10.4292/wjgpt.v6.i1.1PMID: 25664197.