Preparation and Characterization of Immobilized Lipase from Pseudomonas Cepacia onto Magnetic Cellulose Nanocrystals

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Cao, Shi Lin ^a   Huang, Yu Mei ^a   Li, Xue Hui ^a   Xu, Pei ^a   Wu, Hong ^a   Li, Ning ^a   Lou, Wen Yong ^a   Zong, Min Hua ^a  

^a SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CELLULOSE; IMMOBILIZED ENZYME; KETOPROFEN; MAGNETITE NANOPARTICLE; TRIACYLGLYCEROL LIPASE;

BIOCATALYSIS; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; HYDROLYSIS; ISOLATION AND PURIFICATION; METABOLISM; PH; PSEUDOMONAS; TEMPERATURE;

BACTERIAL PROTEINS; BIOCATALYSIS; CELLULOSE; ENZYME STABILITY; ENZYMES, IMMOBILIZED; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KETOPROFEN; LIPASE; MAGNETITE NANOPARTICLES; PSEUDOMONAS; TEMPERATURE;

EID: 84957583866     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep20420     Document Type: Article

References (48)

1. Laura de la Garza, A., Milagro, F. I., Boque, N., Campion, J. & Alfredo Martinez, J. Natural inhibitors of pancreatic lipase as new players in obesity treatment. Planta Med. 77, 773-785 (2011).
2. Garcia-Galan, C., Berenguer-Murcia, A., Fernandez-Lafuente, R. & Rodrigues, R. C. Potential of different enzyme immobilization strategies to improve enzyme performance. Adv. Syn. & Cat. 353, 2885-2904 (2011).
3. Gupta, P., Dutt, K., Misra, S., Raghuwanshi, S. & Saxena, R. K. Characterization of cross-linked immobilized lipase from thermophilic mould thermomyces lanuginosa using glutaraldehyde. Bioresour. Technol. 100, 4074-4076 (2009).
4. Guauque Torres, M. D. P., Foresti, M. L. & Ferreira, M. L. Cross-linked enzyme aggregates (cleas) of selected lipases: a procedure for the proper calculation of their recovered activity. AMB Express. 3, 25-25 (2013).
5. Talekar, S. J. et al. Parameters in preparation and characterization of cross linked enzyme aggregates (CLEAs). Rsc Adv. 3, 12485-12511 (2013).
6. Xiong, R., Zhang, X. X., Tian, D., Zhou, Z. H. & Lu, C. H. Comparing microcrystalline with spherical nanocrystalline cellulose from waste cotton fabrics. Cellulose. 19, 1189-1198 (2012).
7. Incani, V., Danumah, C. & Boluk, Y. Nanocomposites of nanocrystalline cellulose for enzyme immobilization. Cellulose. 20, 191-200 (2013).
8. R., Yang & F., Wang. Peroxidase conjugate of cellulose nanocrystals for the removal of chlorinated phenolic compounds in aqueous solution. Biotechnol. 7, 233-241 (2008).
9. Cao, S.-L., Xu, H., Li, X.-H., Lou, W.-Y. & Zong, M.-H. Papain@magnetic nanocrystalline cellulose nanobiocatalyst: a highly efficient biocatalyst for dipeptide biosynthesis in deep eutectic solvents. ACS Sus. Chem. & Eng. 3, 1589-1599 (2015).
10. Edwards, J. V., Prevost, N. T., Condon, B., French, A. & Wu, Q. Immobilization of lysozyme-cellulose amide-linked conjugates on cellulose I and II cotton nanocrystalline preparations. Cellulose. 19, 495-506 (2012).
11. Cao, S.-L., Li, X.-H., Lou, W.-Y. & Zong, M.-H. Preparation of a novel magnetic cellulose nanocrystal and its efficient use for enzyme immobilization. J. Mater. Chem. B. 2, 5522-5530 (2014).
12. Pollard, D. J. & Woodley, J. M. Biocatalysis for pharmaceutical intermediates: the future is now. Trends Biotechnol. 25, 66-73 (2007).
13. Lai, J.-Q., Hu, Z.-L., Sheldon, R. A. & Yang, Z. Catalytic performance of cross-linked enzyme aggregates of Penicillium expansum lipase and their use as catalyst for biodiesel production. Process Biochem. 47, 2058-2063 (2012).
14. Durand, E., Lecomte, J. & Villeneuve, P. Deep eutectic solvents: Synthesis, application, and focus on lipase-catalyzed reactions. Eur. J. Lip. Sci. Technol. 115, 379-385 (2013).
15. Kartal, F. & Kilinc, A. Crosslinked aggregates of Rhizopus oryzae lipase as industrial biocatalysts: Preparation, optimization, characterization, and application for enantioselective resolution reactions. Biotechnol. Prog. 28, 937-945 (2012).
16. Leung, A. C. et al. Characteristics and properties of carboxylated cellulose nanocrystals prepared from a novel one-step procedure. Small. 7, 302-305 (2011).
17. Xu, Y. & Du, Y. Effect of molecular structure of chitosan on protein delivery properties of chitosan nanoparticles. Int. J. Pharmaceut. 250, 215-226 (2003).
18. Qi, L., Xu, Z., Jiang, X., Hu, C. & Zou, X. Preparation and antibacterial activity of chitosan nanoparticles. Carbohyd. Res. 339, 2693-2700 (2004).
19. Lou, W.-Y., Zong, M.-H., Smith, T. J., Wu, H. & Wang, J.-F. Impact of ionic liquids on papain: an investigation of structure-function relationships. Greem Chem. 8, 509-512 (2006).
20. Lau, R. M. et al. Dissolution of candida antarctica lipase B in ionic liquids: effects on structure and activity. Green Chem. 6, 483-487 (2004).
21. van Rantwijk, F., Secundo, F. & Sheldon, R. A. Structure and activity of Candida antarctica lipase B in ionic liquids. Green Chem. 8, 282-286 (2006).
22. Wang, M. et al. Enhancement of activity of cross-linked enzyme aggregates by a sugar-assisted precipitation strategy: Technical development and molecular mechanism. J. Biotechnol. 156, 30-38 (2011).
23. Eisenberg, D. The discovery of the α-helix and β-sheet, the principal structural features of proteins. P. Nat. Acad. Sci. 100, 11207-11210 (2003).
24. Welsch, N., Becker, A. L., Dzubiella, J. & Ballauff, M. Core-shell microgels as "smart" carriers for enzymes. Soft Mat. 8, 1428-1436 (2012).
25. Huang, S. et al. Protein-coated microcrystals from candida rugosa lipase: its immobilization, characterization, and application in resolution of racemic ibuprofen. Appl. Biochem. Biotechnol. 177, 36-47 (2015).
26. De Diego, T., Lozano, P., Gmouh, S., Vaultier, M. & Iborra, J. L. Understanding structure-stability relationships of candida a ntartica lipase b in ionic liquids. Biomacromolecules. 6, 1457-1464 (2005).
27. Chen, H.-L. & Yokochi, A. X-ray diffractometric study of microcrystallite size of naturally colored cottons. J. Appl. Polym. Sci. 76, 1466-1471 (2000).
28. Liu, H., Jiang, E., Bai, H. & Zheng, R. Structure and magnetotransport properties of Fe3O4-SiO2 composite films reactively sputtered at room temperature. J.Appl. Phy. 95, 5661-5665 (2004).
29. Zhang, G. et al. Lipase immobilized on graphene oxide as reusable biocatalyst. Ind. & Eng. Chem. Res. 53, 19878-19883 (2014).
30. Yu, C.-Y., Li, X.-F., Lou, W.-Y. & Zong, M.-H. Cross-linked enzyme aggregates of mung bean epoxide hydrolases: a highly active, stable and recyclable biocatalyst for asymmetric hydrolysis of epoxides. J. Biotechnol. 166, 12-19 (2013).
31. Cao, L., van Rantwijk, F. & Sheldon, R. A. Cross-linked enzyme aggregates: a simple and effective method for the immobilization of penicillin acylase. Org. Let. 2, 1361-1364 (2000).
32. Polson, C., Sarkar, P., Incledon, B., Raguvaran, V. & Grant, R. Optimization of protein precipitation based upon effectiveness of protein removal and ionization effect in liquid chromatography-tandem mass spectrometry. J. Chromatogr. B. 785, 263-275 (2003).
33. Qiu, G. M., Zhu, B. K. & Xu, Y. Y. α -Amylase immobilized by Fe3O4/poly (styrene-co-maleic anhydride) magnetic composite microspheres: Preparation and characterization. J. Appl. Polym. Sci. 95, 328-335 (2005).
34. Shah, S., Sharma, A. & Gupta, M. N. Preparation of cross-linked enzyme aggregates by using bovine serum albumin as a proteic feeder. Anal. Biochem. 351, 207-213 (2006).
35. Bayramoglu, G., Senkal, B. F., Yilmaz, M. & Arica, M. Y. Immobilization and stabilization of papain on poly(hydroxyethyl methacrylate-ethylenglycol dimethacrylate) beads grafted with epoxy functional polymer chains via surface-initiated-atom transfer radical polymerization (SI-ATRP). Bioresour. Technol. 102, 9833-9837 (2011).
36. Akgöl, S., Kacar, Y., Denizli, A. & Arica, M. Hydrolysis of sucrose by invertase immobilized onto novel magnetic polyvinylalcohol microspheres. Food Chem. 74, 281-288 (2001).
37. Nguyen, Q. D., Rezessy-Szabó, J. M., Czukor, B. & Hoschke, Á. Continuous production of oligofructose syrup from jerusalem artichoke juice by immobilized endo-inulinase. Process Biochem. 46, 298-303 (2011).
38. Jordan, J., Kumar, C. S. & Theegala, C. Preparation and characterization of cellulase-bound magnetite nanoparticles. J Mol Catal B: Enzym. 68, 139-146 (2011).
39. Sangeetha, K. & Abraham, T. E. Chemical modification of papain for use in alkaline medium. J Mol Catal B: Enzym. 38, 171-177 (2006).
40. Khaparde, S. S. & Singhal, R. S. Chemically modified papain for applications in detergent formulations. Bioresour. Technol. 78, 1-4 (2001).
41. Lei, H. et al. The preparation and catalytically active characterization of papain immobilized on magnetic composite microspheres. Enzyme Microb. Tech. 35, 15-21 (2004).
42. Mahmoud, K. A., Lam, E., Hrapovic, S. & Luong, J. H. T. Preparation of well-dispersed gold/magnetite nanoparticles embedded on cellulose nanocrystals for efficient immobilization of papain enzyme. ACS Appl. Mater. & Int. 5, 4978-4985 (2013).
43. Mateo, C., Palomo, J. M., Fernandez-Lorente, G., Guisan, J. M. & Fernandez-Lafuente, R. Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme Microb. Tech. 40, 1451-1463 (2007).
44. Yandri, Y., Susanti, D., Suhartati, T. & Hadi, S. Immobilization of a-amylase from locale bacteria isolate bacillus subtilis itbccb148 with carboxymethyl cellulose (CM-Cellulose). Modern Appl. Sci. 6, 81 (2012).
45. Yilmaz, E., Can, K., Sezgin, M. & Yilmaz, M. Immobilization of candida rugosa lipase on glass beads for enantioselective hydrolysis of racemic naproxen methyl ester. Bioresour. Technol. 102, 499-506 (2011).
46. Kilara, A., Shahani, K. & Wagner, F. W. Preparation and properties of immobilized papain and lipase. Biotechnol. Bioeng. 19, 1703-1714 (1977).
47. Jun Park, H., Choi, W. J., Chul Huh, E., Lee, E. Y. & Choi, C. Y. Production of optically active ketoprofen by direct enzymatic esterification. J. Biosci. Bioeng. 87, 545-547 (1999).
48. Bradford, M. M. A Rapid And Sensitive Method For the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt. Biochem. 72, 248-254 (1976).