Reconstitution of fungal nonribosomal peptide synthetases in yeast and in vitro

Methods in Molecular Biology

Volumn 1401, Issue , 2016, Pages 103-119

  Cacho, Ralph A ^a   Tang, Yi ^a  

^a NONE

Author keywords

Fungal secondary metabolite; Nonribosomal peptide synthetase; Transformation assisted recombination; Yeast protein expression

Indexed keywords

NONRIBOSOMAL PEPTIDE SYNTHETASE; FUNGAL PROTEIN; PEPTIDE SYNTHASE;

AFFINITY CHROMATOGRAPHY; AGAR GEL ELECTROPHORESIS; ASPERGILLUS NIDULANS; CONTROLLED STUDY; FUNGAL CELL CULTURE; FUNGAL STRAIN; HETEROLOGOUS EXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IN VITRO STUDY; INTRON; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PREDICTION; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SACCHAROMYCES CEREVISIAE; START CODON; STOP CODON; YEAST CELL; GENETIC RECOMBINATION; GENETIC TRANSFORMATION; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR CLONING; PLASMID; PROCEDURES;

CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; FUNGAL PROTEINS; PEPTIDE SYNTHASES; PLASMIDS; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, GENETIC;

EID: 84957551948     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-3375-4_7     Document Type: Chapter

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