Flow cytometry approach to quantify the viability of milk somatic cell counts after various physico-chemical treatments

PLoS ONE

Volumn 10, Issue 12, 2015, Pages

  Li, Na ^a,b,c   Richoux, Romain ^c   Perruchot, Marie Hélène ^b,d   Boutinaud, Marion ^b,d   Mayol, Jean François ^e   Gagnaire, Valérie ^a,b  

^a UMR1253   (France)

^b AGROCAMPUS OUEST   (France)

^c Actalia   (France)

^d PEGASE   (France)

^e R and D Transcure Bioservices   (France)

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHATE BUFFERED SALINE; ANTIBODY;

ANIMAL CELL; ARTICLE; CELL COUNT; CELL SUBPOPULATION; CELL VIABILITY; CENTRIFUGATION; CONTROLLED STUDY; FEMALE; FLOW CYTOMETRY; HEAT TREATMENT; JERSEY CATTLE; LYMPHOCYTE; MACROPHAGE; MICROSCOPY; MILK; NEUTROPHIL; NONHUMAN; QUANTITATIVE ANALYSIS; SOMATIC CELL; STORAGE; ANIMAL; BOVINE; CELL SURVIVAL; CYTOLOGY; FOOD QUALITY; IMMUNOLOGY; PROCEDURES; STANDARDS;

ANIMALS; ANTIBODIES; CATTLE; CELL COUNT; CELL SURVIVAL; FEMALE; FLOW CYTOMETRY; FOOD QUALITY; MILK; NEUTROPHILS;

EID: 84956943895     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0146071     Document Type: Article

References (36)

1. Paape MJ, Bannerman DD, Zhao X, Lee JW The bovine neutrophil: structure and function in blood and milk. Vet. Res. 2003; 34: 597-627. doi: 10.1051/vetres:2003024 PMID: 14556697
2. Olechnowicz J, Jaskowski JM Somatic cells count in cow's bulk tank milk. J. Vet. Med. Sci. 2012; 74: 681-686. PMID: 22230979
3. Schwarz D, Diesterbeck US, Konig S, Brugemann K., Schlez K, Zschock M et al. Flow cytometric differential cell counts in milk for the evaluation of inflammatory reactions in clinically healthy and subclinically infected bovine mammary glands. J. Dairy Sci. 2011; 94: 5033-5044. doi: 10.3168/jds.2011-4348 PMID: 21943754
4. Albenzio M, Caroprese M. Differential leucocyte count for ewe milk with low and high somatic cell count. J. Dairy Res. 2011; 78: 43-48. doi: 10.1017/S0022029910000798 PMID: 21134312
5. Piepers S, De Vliegher S, Demeyere K, Lambrecht B.N., De Kruif A, Meyer Eet al. Technical note: flow cytometric identification of bovine milk neutrophils and simultaneous quantification of their viability. J. Dairy Sci. 2009; 92: 626-631. doi: 10.3168/jds.2008-1393 PMID: 19164674
6. Leitner G, Chaffer M, Krifucks O., Glickman A, Ezra E, Saran A. Milk leucocyte populations in heifers free of udder infection. J. Vet. Med. 2000; 47:133-138.
7. Mehne D, Drees S, Schuberth H.J., Sauter-Louis C, Zerbe H, Petzl W. Accurate and rapid flow cytometric leukocyte differentiation in low and high somatic cell count milk. Milchwissenschaft 2010; 65: 235-238.
8. Boulaaba A, Grabowski N, Klein G. Differential cell count of caprine milk by flow cytometry and microscopy. Small Ruminant Res. 2011; 97:117-123.
9. Dumas E, Martel C, Neagoe P.E., Bonnefoy A., Sirois MG. Angiopoietin-1 but not angiopoietin-2 promotes neutrophil viability: role of interleukin-8 and platelet-activating factor. BBA-Mol. Cell Res. 2012; 1823: 358-367.
10. Wauters J, Martens A, Pille F., Dumoulin M, Gasthuys F, Sys S et al. Viability and cell death of synovial fluid neutrophils as diagnostic biomarkers in equine infectious joint disease: a pilot study. Res. Vet. Sci. 2012; 92:132-137. doi: 10.1016/j.rvsc.2010.10.007 PMID: 21056440
11. Li N, Richoux R, Boutinaud M., Martin P, Gagnaire V. Role of somatic cells on dairy processes and products: a review. Dairy Sci. Technol. 2014; 94: 517-538. doi: 10.1007/s13594-014-0176-3 PMID: 25309683
12. Kelly AL, O'Flaherty F, Fox PF. Indigenous proteolytic enzymes in milk: abrief overview of the present state of knowledge. Int. Dairy J. 2006; 16: 563-572.
13. ISO13366-2/IDF148-2. Milk-Enumeration of somatic cells - Part 2: guidance on the operation offluoro-opto-electronic counters. International Dairy Federation Standard 2006; 1-13.
14. Council Directive 86/609/EEC. Protection of animals used for experimental and other scientific purposes. The Council of the European Communities 1986; 1-7.
15. Sharma N, Rho GJ, Hong Y.H., Kang TY, Lee HK, Hur TY et al. Bovine mastitis: an Asian perspective. Asian. J. Anim. Vet. Adv. 2012; 7: 454-476.
16. Sladek Z, Rysanek D. Cell death and CD14 expression in resident and inflammatory polymorphonuclear leukocytes from virgin bovine mammary gland. Res. Vet. Sci. 2011; 90: 226-234. doi: 10.1016/j.rvsc.2010.06.017 PMID: 20599238
17. Asai K, Komine Y, Kozutsumi T., Yamaguchi T, Komine K, Kumagai K. Predominant subpopulations of T lymphocytes in the mammary gland secretions during lactation and intraepithelial T lymphocytes in the intestine of dairy cows. Veterinary Immunology and Immunopathology 2000; 73: 233-240. PMID: 10713337
18. Blagitz MG, Souza FN, Batista C.F., Diniz SA, Azevedo LFF, Silva MX et al. Flow cytometric analysis: Interdependence of healthy and infected udder quarters. J. Dairy Sci. 2015; 98: 2401-2408. doi: 10.3168/jds.2014-8727 PMID: 25682144
19. Blagitz MG, Souza FN, Batista C.F., Azevedo LFF, Benites NR, Melville PA et al. The neutrophil function and lymphocyte profile of milk from bovine mammary glands infected with Streptococcus dysgalactiae. J. Dairy Res. 2015; in press available on CJO2015. doi:10.1017/S0022029915000308
20. Harp JA, Stabel JR, Pesch B.A., Goff JP. Expression of adhesion molecules on milk and blood lymphocytes from periparturient dairy cattle with Johne's disease. Veterinary Immunology and Immunopathology 2004; 98: 69-76. PMID: 15127843
21. Kehrli ME, Harp JA Immunity in the mammary gland. Veterinary Clinics of North America-Food Animal Practice 2001; 17:495-516.
22. la Libera AMMP, de Souza FN, Batista CF, Santos BP, deAzevedo LFF, Sanchez EMR et al. Effects of bovine leukemia virus infection on milk neutrophil function and the milk lymphocyte profile. Vet. Res. 2015; 46.
23. Lun S, Astrom G, Magnusson U., Ostensson K. Total and differential leucocyte counts and lymphocyte subpopulations in lymph, afferent and efferent to the supramammary lymph node, during endotoxin-induced bovine mastitis. Reproduction in Domestic Animals 2007; 42:126-134. PMID: 17348968
24. Yamaguchi T, Hiratsuka M, Asai K., Kai K, Kumagai K. Differential distribution of T lymphocyte subpopulations in the bovine mammary gland during lactation. J. Dairy Sci. 1999; 82:1459-1464. PMID: 10416161
25. Paape MJ, Lilius EM, Wiitanen P.A., Kontio MP, Miller RH. Intramammary defense against infections induced by Escherichia coli in cows. American Journal of Veterinary Research 1996; 57: 477-482. PMID: 8712510
26. Sladek Z, Ryznarova H, Rysanek D. Macrophages of the bovine heifer mammary gland: Morphological features during initiation and resolution of the inflammatory response. Anatomia Histologia Embryologia-Journal of Veterinary Medicine Series C 2006; 35:116-124.
27. Lee JW, Paape MJ, Elsasser T.H., Zhao X. Elevated milk soluble CD14 in bovine mammary glands challenged with Escherichia coli lipopolysaccharide. J. Dairy Sci. 2003; 86: 2382-2389. PMID: 12906056
28. Burton JL, Erskine RJ Immunity and mastitis - Some new ideas for an old disease. Veterinary Clinics of North America-Food Animal Practice 2003; 19:1-+.
29. Sladek Z, Rysanek D. The role of CD14 during resolution of experimentally induced Staphylococcus aureus and Streptococcus uberis mastitis. Comparative Immunology Microbiology and Infectious Diseases 2006; 29: 243-262.
30. Paape MJ, Wergin WP, Guidry A.J., Pearson RE. Leukocytes-second line of defense against invading mastitis pathogens. J. Dairy Sci. 1979; 62:135-153. PMID: 379058
31. Boisclair G. Evaluation de la santé de la vache après le vêlage. Med. Vet. 2007; 1: 45-47.
32. Larsen LB, Wium H, Benfeldt C., Heegaard CW, Ardo Y, Qvist KB et al. Bovine milk procathepsin D: presence and activity in heated milk and in extracts of rennet-free UF-Feta cheese. Int. Dairy J. 2000; 10: 67-73. doi:10.1016/S0958-6946(00)00020-0
33. Caroprese M, Marzano A, Schena L., Marino R, Santillo A, Albenzio M. Contribution of macrophages to proteolysis and plasmin activity in ewe bulk milk. J. Dairy Sci. 2007; 90: 2767-2772. doi: 10.3168/jds.2006-691 PMID: 17517716
34. Wang DIC, Sinskey TJ Effect of centrifugation on the viability of burkitt lymphoma cells. Biotechnol. Bioeng. 1968; 10:641-649.
35. Capiaumont J, Legrand C, Dousset B., Parmentelot I, Linden G, Belleville F et al. Whey and β-lactoglobulin: 2 milk by-products which can replace fetal calf serum in mouse hybridomacell culture. Lait 1994; 74:127-137. doi: 10.1051/lait:1994211
36. FAO (1998) Le lait et les produits laitiers dans la nutrition humaine - Produits laitiers: consommation, technologie et microbiologie. Rome, Italy: FAO.