Technical note: Flow cytometric identification of bovine milk neutrophils and simultaneous quantification of their viability

Journal of Dairy Science

Volumn 92, Issue 2, 2009, Pages 626-631

  Piepers, S ^a   De Vliegher, S ^a   Demeyere, K ^a   Lambrecht, B N ^a   De Kruif, A ^a   Meyer, E ^a   Opsomer, G ^a  

^a GHENT UNIVERSITY   (Belgium)

Author keywords

Bovine; Flow cytometry; Milk; Neutrophil

Indexed keywords

ALEXA; BOVINAE;

EID: 59349120800     PISSN: 00220302     EISSN: 15253198     Source Type: Journal    
DOI: 10.3168/jds.2008-1393     Document Type: Article

References (25)

1. Boutet, P., D. Boulanger, L. Gillet, A. Vanderplasschen, R. Closset, F. Bureau, and P. Lekeux. 2004. Delayed neutrophil apoptosis in bovine subclinical mastitis. J. Dairy Sci. 87:4104-4114.
2. Davis, W. C., S. Marusic, H. A. Lewin, G. A. Splitter, L. E. Perryman, T. C. Mcguire, and J. S. Gorham. 1987. The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other species. Vet. Immunol. Immunopathol. 15:337-376.
3. Dohoo, I., M. Wayne, and H. Stryhn. 2003. Veterinary Epidemiologic Research. AVC Inc., Charlottetown, Prince Edward Island, Canada.
4. Dosogne, H., F. Vangroenweghe, J. Mehrzad, A. M. Massart-Leën, and C. Burvenich. 2003. Differential leukocyte count method for bovine low somatic cell count milk. J. Dairy Sci. 86:828-834.
5. Feng, W., and X. D. Zheng. 2005. Comparing techniques for detecting the number of somatic cells in raw milk. Eur. Food Res. Technol. 220:653-657.
6. Keeney, M., J. W. Gratama, I. H. Chin-Yee, and D. R. Sutherland. 1998. Isotype controls in the analysis of lymphocytes and CD34+ stem and progenitor cells by flow cytometry - Time to let go! Cytom. B 34:280-283.
7. Koess, C., and J. Hamann. 2008. Detection of mastitis in the bovine mammary gland by flow cytometry at early stages. J. Dairy Res. 75:225-232.
8. Lakowicz, J. R. 1999. Principles of Fluorescence Spectroscopy. 2nd ed. Kluwer Academic/Plenum, New York, NY.
9. Lamote, I., E. Meyer, L. Duchateau, and C. Burvenich. 2004. Influence of 17β-estradiol, progesterone, and dexamethasone on diapedesis and viability of bovine blood polymorphonuclear leukocytes. J. Dairy Sci. 87:3340-3349.
10. Mehrzad, J., H. Dosogne, E. Meyer, R. Heyneman, and C. Burvenich. 2001. Respiratory burst activity of blood and milk neutrophils in dairy cows during different stages of lactation. J. Dairy Res. 68:399-415.
11. Mehrzad, J., L. Duchateau, and C. Burvenich. 2004. Viability of milk neutrophils and severity of bovine coliform mastitis. J. Dairy Sci. 87:4150-4162.
12. Mehrzad, J., L. Duchateau, S. Pyörälä, and C. Burvenich. 2002. Blood and milk neutrophil chemiluminescence and viability in primiparous and pluriparous dairy cows during late pregnancy around parturition and early lactation. J. Dairy Sci. 85:3268-3276.
13. Miller, R. H., M. J. Paape, R. Filep, and S. Link. 1993. Flow cytometric analysis of neutrophils in cows' milk. Am. J. Vet. Res. 54:1975-1979.
14. O'Garmon, M. R. G., and J. Thomas. 1999. Isotype controls - Time to let go? Cytom. Part B 38:78-80.
15. Ontsouka, C. E., R. M. Bruckmaier, and J. W. Blum. 2003. Fractionized milk composition during removal of colostrums and mature milk. J. Dairy Sci. 6:2005-2011.
16. Paape, M. J., E. M. Lilius, P. A. Wiitanen, M. P. Kontio, and R. H. Miller. 1996. Intramammary defense against infections induced by Escherichia coli in cows. Am. J. Vet. Res. 57:477-482.
17. Pulgarín, J. A. M., A. A. Molina, and M. T. A. Pardo. 2005. Fluorescence characteristics of several whey samples subjected to different treatments and conditions. Anal. Chim. Acta 536:153-158.
18. Rainard, P., and C. Riollet. 2006. Innate immunity of the bovine mammary gland. Vet. Res. 37:369-400.
19. Riollet, C., P. Rainard, and B. Poutrel. 2001. Cell subpopulations and cytokine expression in cow milk in response to chronic Staphylococcus aureus infection. J. Dairy Sci. 84:1077-1084.
20. Rivas, A. L., R. Tadevosyan, F. W. Quimby, T. Coksaygan, and D. H. Lein. 2002. Identification of subpopulations of bovine mammary gland phagocytes and evaluation of sensitivity and specificity of morphologic and functional indicators of bovine mastitis. Can. J. Vet. Res. 66:165-172.
21. Sládek, Z., and D. Ryšanek. 2005. Neutrophil apoptosis during the resolution of bovine mammary gland injury. Res. Vet. Sci. 70:41-46.
22. van Eeden, S. F., M. E. Klut, B. A. M. Walker, and J. C. Hogg. 1999. The use of flow cytometry to measure neutrophil function. J. Immunol. Methods 232:23-43.
23. Van Oostveldt, K., M. J. Paape, H. Dosogne, and C. Burvenich. 2002. Effect of apoptosis on phagocytosis, respiratory burst and CD18 adhesion receptor expression of bovine neutrophils. Domest. Anim. Endocrinol. 22:37-50.
24. Van Oostveldt, K., F. Vangroenweghe, H. Dosogne, and C. Burvenich. 2001. Apoptosis and necrosis of blood and milk polymorphonuclear leukocytes in early and midlactating healthy cows. Vet. Res. 32:617-622.
25. Vermes, I., C. Haanen, H. Stefferns-Nakken, and C. Reutelingsperger. 1995. A novel assay for apoptosis: Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin-V. J. Immunol. Methods 184:39-51.