Data on the standardization of a cyclohexanone-responsive expression system for Gram-negative bacteria

Data in Brief

Volumn 6, Issue , 2016, Pages 738-744

  Benedetti, Ilaria ^a   Nikel, Pablo I ^a   de Lorenzo, Víctor ^a  

^a CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

Author keywords

Escherichia coli; Expression system; Heterologous gene expression; Metabolic engineering; Pseudomonas putida; Synthetic biology

Indexed keywords

BIODEGRADATION; GENETIC ALGORITHMS; GENETIC PROGRAMMING; METABOLIC ENGINEERING; STANDARDIZATION; SYNTHETIC BIOLOGY; TRANSCRIPTION;

ACINETOBACTER JOHNSONII; CYCLOHEXANONES; EXPRESSION SYSTEM; GENES EXPRESSION; GENETIC TOOLS; GRAM-NEGATIVE BACTERIA; HETEROLOGOUS GENE EXPRESSIONS; MICROBIAL CELL FACTORIES; PSEUDOMONAS PUTIDA; SYNTHETIC BIOLOGY;

ESCHERICHIA COLI;

EID: 84956522448     PISSN: None     EISSN: 23523409     Source Type: Journal    
DOI: 10.1016/j.dib.2016.01.022     Document Type: DP

References (20)

1. Benedetti I., de Lorenzo V., Nikel P.I. Genetic programming of catalytic Pseudomonas putida biofilms for boosting biodegradation of haloalkanes. Metab. Eng. 2016, 33:109-118.
2. Silva-Rocha R., Martínez-García E., Calles B., Chavarría M., Arce-Rodríguez A., de las Heras A., Páez-Espino A.D., Durante-Rodríguez G., Kim J., Nikel P.I., Platero R., de Lorenzo V. The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes. Nucleic Acids Res. 2013, 41:D666-D675.
3. Green M.R., Sambrook J. Molecular Cloning: A Laboratory Manual 2012, Cold Spring Harbor Laboratory, Cold Spring Harbor. 4th ed.
4. Nikel P.I., Chavarría M., Martínez-García E., Taylor A.C., de Lorenzo V. Accumulation of inorganic polyphosphate enables stress endurance and catalytic vigour in Pseudomonas putida KT2440. Microb. Cell Fact. 2013, 12:50.
5. Nikel P.I., de Lorenzo V. Implantation of unmarked regulatory and metabolic modules in Gram-negative bacteria with specialised mini-transposon delivery vectors. J. Biotechnol. 2013, 163:143-154.
6. Nikel P.I., de Lorenzo V. Engineering an anaerobic metabolic regime in Pseudomonas putida KT2440 for the anoxic biodegradation of 1,3-dichloroprop-1-ene. Metab. Eng. 2013, 15:98-112.
7. Nikel P.I., de Almeida A., Pettinari M.J., Méndez B.S. The legacy of HfrH: mutations in the two-component system CreBC are responsible for the unusual phenotype of an Escherichia coli arcA mutant. J. Bacteriol. 2008, 190:3404-3407.
8. Nikel P.I., Kim J., de Lorenzo V. Metabolic and regulatory rearrangements underlying glycerol metabolism in Pseudomonas putida KT2440. Environ. Microbiol. 2014, 16:239-254.
9. Martínez-García E., Nikel P.I., Chavarría M., de Lorenzo V. The metabolic cost of flagellar motion in Pseudomonas putida KT2440. Environ. Microbiol. 2014, 16:291-303.
10. Cheng Q., Thomas S.M., Kostichka K., Valentine J.R., Nagarajan V. Genetic analysis of a gene cluster for cyclohexanol oxidation in Acinetobacter sp. strain SE19 by in vitro transposition. J. Bacteriol. 2000, 182:4744-4751.
11. Iwaki H., Hasegawa Y., Teraoka M., Tokuyama T., Bergeron H., Lau P.C. Identification of a transcriptional activator (ChnR) and a 6-oxohexanoate dehydrogenase (ChnE) in the cyclohexanol catabolic pathway in Acinetobacter sp. strain NCIMB 9871 and localization of the genes that encode them. Appl. Environ. Microbiol. 1999, 65:5158-5162.
12. Steigedal M., Valla S. The Acinetobacter sp. chnB promoter together with its cognate positive regulator ChnR is an attractive new candidate for metabolic engineering applications in bacteria. Metab. Eng. 2008, 10:121-129.
13. Letek M., Valbuena N., Ramos A., Ordóñez E., Gil J.A., Mateos L.M. Characterization and use of catabolite-repressed promoters from gluconate genes in Corynebacterium glutamicum. J. Bacteriol. 2006, 188:409-423.
14. Miller W.G., Lindow S.E. An improved GFP cloning cassette designed for prokaryotic transcriptional fusions. Gene 1997, 191:149-153.
15. Martínez-García E., Aparicio T., de Lorenzo V., Nikel P.I. New transposon tools tailored for metabolic engineering of Gram-negative microbial cell factories. Front. Bioeng. Biotechnol. 2014, 2:46.
16. Nikel P.I., Romero-Campero F.J., Zeidman J.A., Goñi-Moreno A., de Lorenzo V. The glycerol-dependent metabolic persistence of Pseudomonas putida KT2440 reflects the regulatory logic of the GlpR repressor. mBio 2015, 6:e00340-00315.
17. Martínez-García E., Aparicio T., Goñi-Moreno A., Fraile S., de Lorenzo V. SEVA 2.0: an update of the Standard European Vector Architecture for de-/re-construction of bacterial functionalities. Nucleic Acids Res. 2014, 43:D1183-D1189.
18. Donoghue N.A., Trudgill P.W. The metabolism of cyclohexanol by Acinetobacter NCIB 9871. Eur. J. Biochem. 1975, 60:1-7.
19. Herrero M., de Lorenzo V., Timmis K.N. Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in Gram-negative bacteria. J. Bacteriol. 1990, 172:6557-6567.
20. Datsenko K.A., Wanner B.L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. USA 2000, 97:6640-6645.