The Acinetobacter sp. chnB promoter together with its cognate positive regulator ChnR is an attractive new candidate for metabolic engineering applications in bacteria

Metabolic Engineering

Volumn 10, Issue 2, 2008, Pages 121-129

  Steigedal, Magnus ^a   Valla, Svein ^a  

^a NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Norway)

Author keywords

ChnB; ChnR; Metabolic engineering; Pm; Promoter; XylS

Indexed keywords

COGNITIVE SYSTEMS; DATA STRUCTURES; ESCHERICHIA COLI; MONOMERS;

EXPRESSION CASSETTE; METABOLIC ENGINEERING; MONOMER COMPOSITION;

BACTERIA;

ALGINIC ACID; BACTERIAL PROTEIN; CYCLOHEXANONE; EXOPOLYSACCHARIDE; LUCIFERASE; PROTEIN CHNB; UNCLASSIFIED DRUG;

ACINETOBACTER; ARTICLE; ESCHERICHIA COLI; GENE CASSETTE; GENE EXPRESSION; GENE EXPRESSION REGULATION; METABOLIC ENGINEERING; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PSEUDOMONAS FLUORESCENS; REGULATOR GENE; REPLICON; REPORTER GENE; SPECIES;

ACINETOBACTER SP.; ESCHERICHIA COLI; PSEUDOMONAS FLUORESCENS;

EID: 39749182221     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2007.08.002     Document Type: Article

References (29)

1. K. Bakkevig H. Sletta M. Gimmestad R. Aune H. Ertesvåg K. Degnes B.E. Christensen T.E. Ellingsen S. Valla Role of the Pseudomonas fluorescens alginate lyase (AlgL) in clearing the periplasm of alginates not exported to the extracellular environment J. Bacteriol. 187 2005 8375 8384
2. J.M. Blatny T. Brautaset H.C. Winther-Larsen K. Haugan S. Valla Construction and use of a versatile set of broad-host-range cloning and expression vectors based on the RK2 replicon Appl. Environ. Microbiol. 63 1997 370 379
3. J.M. Blatny T. Brautaset H.C. Winther-Larsen P. Karunakaran S. Valla Improved broad-host-range RK2 vectors useful for high and low regulated gene expression levels in gram-negative bacteria Plasmid 38 1997 35 51
4. C. Bro J. Nielsen Impact of ‘ome’ analyses on inverse metabolic engineering Metab. Eng. 6 2004 204 211
5. A.Y. Chan M.M. Chan H.M. Lo Y.C. Leung B.L. Lim A dual protein expression system in Bacillus subtilis Protein Expr. Purif. 26 2002 337 342
6. Q. Cheng S.M. Thomas K. Kostichka J.R. Valentine V. Nagarajan Genetic analysis of a gene cluster for cyclohexanol oxidation in Acinetobacter sp . Strain SE19 by in vitro transposition J. Bacteriol. 182 2000 4744 4751
7. L.C. Chew T.M. Ramseier D.M. Retallack J.C. Shneider C.H. Squires H.W. Talbot Production of recombinant proteins G. Gellissen Production of Recombinant Proteins: Novel Microbial and Eucaryotic Expression Systems 2005 Wiley Weinheim 45 65
8. V. de Lorenzo I. Cases M. Herrero K.N. Timmis Early and late responses of TOL promoters to pathway inducers: identification of postexponential promoters in Pseudomonas putida with lacZ-tet bicistronic reporters J. Bacteriol. 175 1993 6902 6907
9. N.A. Donoghue P.W. Trudgill The metabolism of cyclohexanol by Acinetobacter NCIB 9871 Eur. J. Biochem. 60 1975 1 7
10. H. Ertesvåg G. Skjåk-Bræk Modification of alginate using mannuronan C-5-epimerases C. Bucke Methods in Biotechnology 10, Carbohydrate Biotechnology Protocols 1999 Humana Press Totowa, NJ 71 78
11. D.A. Fell Increasing the flux in metabolic pathways: a metabolic control analysis perspective Biotechnol. Bioeng. 58 1998 121 124
12. M. Gimmestad H. Sletta H. Ertesvåg K. Bakkevig S. Jain S.J. Suh G. Skjåk-Bræk T.E. Ellingsen D.E. Ohman S. Valla The Pseudomonas fluorescens AlgG protein, but not its mannuronan C-5-epimerase activity, is needed for alginate polymer formation J. Bacteriol. 185 2003 3515 3523
13. Gimmestad, M., Sletta, H., Karunakaran, P., Bakkevig, K., Ertesvåg, H., Ellingsen, T., Skjåk-Bræk, G., Valla, S., 2004. New mutant strains of Pseudomonas fluorescens and variants thereof, methods for their production, and uses thereof in alginate production. Patent WO 2004/011628 A1.
14. H. Grasdalen High-field, 1H-n.m.r. spectroscopy of alginate: sequential structure and linkage conformations Carbohydr. Res. 118 1983 255 260
15. H. Grasdalen B. Larsen O. Smidsrød Pmr study of the composition and sequence of uronate residues in alginates Carbohydr. Res. 68 1979 23 31
16. M. Herrero V. de Lorenzo B. Ensley K.N. Timmis A T7 RNA polymerase-based system for the construction of Pseudomonas strains with phenotypes dependent on Tol-meta pathway effectors Gene 134 1993 103 106
17. H. Iwaki Y. Hasegawa M. Teraoka T. Tokuyama H. Bergeron P.C. Lau Identification of a transcriptional activator (ChnR) and a 6-oxohexanoate dehydrogenase (ChnE) in the cyclohexanol catabolic pathway in Acinetobacter sp. Strain NCIMB 9871 and localization of the genes that encode them Appl. Environ. Microbiol. 65 1999 5158 5162
18. S. Jain M.J. Franklin H. Ertesvåg S. Valla D.E. Ohman The dual roles of AlgG in C-5-epimerization and secretion of alginate polymers in Pseudomonas aeruginosa Mol. Microbiol. 47 2003 1123 1133
19. M.J. Kang S.H. Yoon Y.M. Lee S.H. Lee J.E. Kim K.H. Jung Y.C. Shin S.W. Kim Enhancement of lycopene production in Escherichia coli by optimization of the lycopene synthetic pathway J. Microbiol. Biotech. 15 2005 880 886
20. T.D. Landry L. Chew J.W. Davis N. Frawley H.H. Foley S.J. Stelman J. Thomas J. Wolt D.S. Hanselman Safety evaluation of an alpha-amylase enzyme preparation derived from the archaeal order Thermococcales as expressed in Pseudomonas fluorescens biovar I Regul. Toxicol. Pharmacol. 37 2003 149 168
21. J. Sambrook D. Russell Molecular Cloning: A Laboratory Manual third ed. 2001 Cold Spring Harbor Laboratory Press New York
22. R. Simon U. Priefer A. Pühler A broad host range mobilization system for in vivo engineering: transposons mutagenesis in gram-negative bacteria Bio/Technology 1 1983 784 791
23. H. Sletta A. Nedal T.E. Aune H. Hellebust S. Hakvåg R. Aune T.E. Ellingsen S. Valla T. Brautaset Broad-host-range plasmid pJB658 can be used for industrial-level production of a secreted host-toxic single-chain antibody fragment in Escherichia coli Appl. Environ. Microbiol. 70 2004 7033 7039
24. H. Sletta A. Tøndervik S. Hakvåg T.E. Aune A. Nedal R. Aune G. Evensen S. Valla T.E. Ellingsen T. Brautaset The presence of N-terminal secretion signal sequences leads to strong stimulation of the total expression levels of three tested medically important proteins during high-cell-density cultivations of Escherichia coli Appl. Environ. Microbiol. 73 2007 906 912
25. G. Stephanopoulos T.W. Simpson Flux amplification in complex metabolic networks Chem. Eng. Sci. 52 1997 2607 2627
26. R. Tobes J.L. Ramos AraC-XylS database: a family of positive transcriptional regulators in bacteria Nucleic Acids Res. 30 2002 318 321
27. S.J. van Dien S. Keyhani C. Yang J.D. Keasling Manipulation of independent synthesis and degradation of polyphosphate in Escherichia coli for investigation of phosphate secretion from the cell Appl. Environ. Microbiol. 63 1997 1689 1695
28. H.C. Winther-Larsen J.M. Blatny B. Valand T. Brautaset S. Valla Pm promoter expression mutants and their use in broad-host-range RK2 plasmid vectors Metab. Eng. 2 2000 92 103
29. H.C. Winther-Larsen K.D. Josefsen T. Brautaset S. Valla Parameters affecting gene expression from the Pm promoter in gram-negative bacteria Metab. Eng. 2 2000 79 91