Effect of Manuka honey gel on the transforming growth factor β1 and β3 concentrations, bacterial counts and histomorphology of contaminated full-thickness skin wounds in equine distal limbs

Australian Veterinary Journal

Volumn 94, Issue 1-2, 2016, Pages 27-34

  Bischofberger, A S ^a   Dart, C M ^a   Horadagoda, N ^a   Perkins, N R ^b   Jeffcott, L B ^a   Little, C B ^c   Dart, A J ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

^b AUSVET ANIMAL HEALTH SERVICES   (Australia)

^c ROYAL NORTH SHORE HOSPITAL   (Australia)

Author keywords

Horses; Manuka honey gel; Skin wounds; Transforming growth factor beta

Indexed keywords

TRANSFORMING GROWTH FACTOR BETA1; TRANSFORMING GROWTH FACTOR BETA3;

ANIMAL; BACTERIAL LOAD; FECES; HONEY; HORSE; HORSE DISEASES; INJURIES; LIMB; METABOLISM; MICROBIOLOGY; SKIN; TOPICAL DRUG ADMINISTRATION; WOUND HEALING;

ADMINISTRATION, TOPICAL; ANIMALS; BACTERIAL LOAD; EXTREMITIES; FECES; HONEY; HORSE DISEASES; HORSES; SKIN; TRANSFORMING GROWTH FACTOR BETA1; TRANSFORMING GROWTH FACTOR BETA3; WOUND HEALING;

EID: 84955586232     PISSN: 00050423     EISSN: 17510813     Source Type: Journal    
DOI: 10.1111/avj.12405     Document Type: Article

References (27)

1. Theoret CL. The pathophysiology of wound repair. Vet Clin North Am Equine Pract 2005;21:1-13.
2. Theoret CL, Barber SM, Moyana TN et al. Expression of transforming growth factor beta(1), beta(3), and basic fibroblast growth factor in full-thickness skin wounds of equine limbs and thorax. Vet Surg 2001;30:269-277.
3. Theoret CL, Barber SM, Gordon JR. Temporal localization of immunoreactive transforming growth factor beta1 in normal equine skin and in full-thickness dermal wounds. Vet Surg 2002;31:274-280.
4. Theoret CL, Barber SM, Moyana TN et al. Preliminary observations on expression of transforming growth factors beta 1 and beta 3 in equine full-thickness skin wounds healing normally or with exuberant granulation tissue. Vet Surg 2002;31:266-273.
5. Farstvedt E, Stashak TS, Othic A. Update on topical wound medications. Clin Tech Equine Pract 2004;3:164-172.
6. Dart AJ, Dowling BA, Smith CL. Topical treatments in equine wound management. Vet Clin North Am Equine Pract 2005;21:77-89.
7. Tonks AJ, Dudley E, Porter NG et al. A 5.8-kDa component of Manuka honey stimulates immune cells via TLR4. J Leuk Biol 2007;82:1147-1155.
8. Tonks A, Cooper RA, Price AJ et al. Stimulation of TNF-alpha release in monocytes by honey. Cytokine 2001;14:240-242.
9. Tonks AJ, Cooper RA, Jones KP et al. Honey stimulates inflammatory cytokine production from monocytes. Cytokine 2003;21:242-247.
10. Blair S, Cokcetin NN, Harry EJ et al. The unusual antibacterial activity of medical-grade leptospermum honey: antibacterial spectrum, resistance and transcriptome analysis. Eur J Clin Microbiol Infect Dis 2009;28:1199-1208.
11. Molan PC. The evidence supporting the use of honey as a wound dressing. Int J Lower Extrem Wounds 2006;5:40-54.
12. Simon A, Traynor K, Santos K et al. Medical honey for wound care: still the latest resort. Evid Based Complement Altern Med 2009;6:165-173.
13. Bischofberger AS, Dart CM, Perkins NR et al. A preliminary study on the effect of Manuka honey on second intention healing of wounds on distal forelimbs in horses. Vet Surg 2012;40:898-902.
14. Bischofberger AS, Dart CM, Perkins NR et al. The effect of short and longer term treatment with Manuka honey on second intention healing of contaminated and uncontaminated wounds on the distal aspect of the forelimbs in horses. Vet Surg 2013;42:154-160.
15. Dart AJ, Perkins NR, Dart CM et al. Effect of bandaging on second intention healing of wounds of the distal limb in horses. Aust Vet J 2009;87:215-218.
16. Lammers R, Henry C, Howell, J. Bacterial counts in experimental, contaminated crush wound irrigated with various concentration of cefazolin and penicillin. Am J Emerg Med 2001;19:1-5.
17. Greenhalgh DG, Sprugel KH, Murray MJ et al. PDGF and FGF stimulate wound healing in the genetically diabetic mouse. Am J Pathol 1990;136:1235-1246.
18. Benjamini Y, Hochberg Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing. J R Stat Soc Series B Methodol 1995;57:289-300.
19. Nisbet HO, Nisbet C, Yarim M et al. Effects of three types of honey on cutaneous wound healing. Wounds 2010;22:275-283.
20. Molan PC. The antibacterial activity of honey. 1: the nature of the antibacterial activity. Bee World 1992;73:5-28.
21. Knottenbelt DC. Basic wound management. In: Knottenbelt DC, editor. Handbook of equine wound management. 3rd edn. Saunders, London, 2003;54-73.
22. Bigbie RB, Schumacher J, Swaim SF et al. effects of amnion and live yeast-cell derivative on 2nd-intention healing in horses. Am J Vet Res 1991;52:1376-1382.
23. Wilmink JM, van Weeren PR. Second-intention repair in the horse and pony and management of exuberant granulation tissue. Vet Clin North Am Equine Pract 2005;21:15-32.
24. Celeste CJ, Deschene K, Riley CB et al. Regional differences in wound oxygenation during normal healing in an equine model of cutaneous fibroproliferative disorder. Wound Repair Regen 2011;19:89-97.
25. Lepault E, Celeste C, Dore M. Comparative study on microvascular occlusion and apoptosis in body and limb wounds in the horse. Wound Repair Regen 2005;13:520-529.
26. Hakkinen L, Westermarck J, Kahari VM et al. Human granulation tissue fibroblasts show enhanced proteoglycan gene expression and altered response to TGF-β1. J Dent Res 1996;75:1767-1778.
27. Falanga V, Qian SW, Danielpour D et al. Hypoxia upregulates the synthesis of TGF-β1 by human dermal fibroblasts. J Invest Dermatol 1991;97:634-637.