Screening Pools of Compounds against Multiple Endogenously Expressed Targets in a Chemoproteomics Binding Assay

Journal of Laboratory Automation

Volumn 21, Issue 1, 2016, Pages 133-142

  Salzer, Elsa ^a   Nixon, Elizabeth ^b   Drewes, Gerard ^a   Reinhard, Friedrich ^a   Bergamini, Giovanna ^a   Rau, Christina ^a  

^a GLAXOSMITHKLINE   (Germany)

^b GLAXOSMITHKLINE   (United States)

Author keywords

acoustic dispenser; chemoproteomics; compound pooling; high throughput screening (HTS)

Indexed keywords

ASSAYS; BINS; COST REDUCTION; MIXTURES; THROUGHPUT;

ASSAY TECHNOLOGIES; CHEMOPROTEOMICS; COMPOUND INTERFERENCE; COMPOUND POOLING; CREATION PROCESS; FALSE POSITIVE/NEGATIVE; HIGH THROUGHPUT; HIGH-THROUGHPUT SCREENING;

LAKES;

MAMMALIAN TARGET OF RAPAMYCIN; PHOSPHATIDYLINOSITOL 3 KINASE; PHOSPHATIDYLINOSITOL 3 KINASE DELTA; PHOSPHATIDYLINOSITOL 3 KINASE GAMMA; PROTEIN KINASE; TANK BINDING KINASE 1; UNCLASSIFIED DRUG; CELL EXTRACT; PROTEIN BINDING; PROTEOME; SOLUTION AND SOLUBILITY;

ACOUSTIC DISPENSING; ACOUSTIC TECHNOLOGY; ARTICLE; BINDING ASSAY; CHEMOPROTEOMICS; CONTROLLED STUDY; HUMAN; HUMAN CELL; PROTEOMICS; ACOUSTICS; CHEMISTRY; HIGH THROUGHPUT SCREENING; LABORATORY AUTOMATION; MEDICAL TECHNOLOGY; PRECLINICAL STUDY; PROCEDURES; SOLUTION AND SOLUBILITY;

ACOUSTICS; AUTOMATION, LABORATORY; BIOMEDICAL TECHNOLOGY; CELL EXTRACTS; DRUG EVALUATION, PRECLINICAL; HIGH-THROUGHPUT SCREENING ASSAYS; PROTEIN BINDING; PROTEOME; SOLUTIONS;

EID: 84955482431     PISSN: 22110682     EISSN: 22110690     Source Type: Journal    
DOI: 10.1177/2211068215595355     Document Type: Article

References (18)

1. M.BantscheffD.EberhardY.Abraham. Quantitative Chemical Proteomics Reveals Mechanisms of Action of Clinical ABL Kinase Inhibitors. Nat. Biotechnol. 2007, 25, 1035–1044.
2. M.BantscheffC.HopfM.M.Savitski. Chemoproteomics Profiling of HDAC Inhibitors Reveals Selective Targeting of HDAC Complexes. Nat. Biotechnol. 2011, 29, 255–265.
3. G.BergaminiK.BellS.Shimamura. A Selective Inhibitor Reveals PI3Kgamma Dependence of T(H)17 Cell Differentiation. Nat. Chem. Biol. 2012, 8, 576–582.
4. R.MacarronM.N.BanksD.Bojanic. Impact of High-Throughput Screening in Biomedical Research. Nat. Rev. Drug Discov. 2011, 10, 188–195.
5. P.SzymanskiM.MarkowiczE.Mikiciuk-OlasikAdaptation of High-Throughput Screening in Drug Discovery-Toxicological Screening Tests. Int. J. Mol. Sci. 2012, 13, 427–452.
6. R.P.HertzbergA.J.PopeHigh-Throughput Screening: New Technology for the 21st Century. Curr. Opin. Chem. Biol. 2000, 4, 445–451.
7. L.L.ElkinD.G.HardenS.Saldanha. Just-in-Time Compound Pooling Increases Primary Screening Capacity without Compromising Screening Quality. J. Biomol. Screen. 2015, 20, 577–587.
8. N.MotlekarS.L.DiamondA.D.NapperEvaluation of an Orthogonal Pooling Strategy for Rapid High-Throughput Screening of Proteases. Assay Drug Dev. Technol. 2008, 6, 395–405.
9. R.M.KainkaryamP.J.WoolfPooling in High-Throughput Drug Screening. Curr. Opin. Drug Discov. Dev. 2009, 12, 339–350.
10. Y.EtzionA.J.MuslinThe Application of Phenotypic High-Throughput Screening Techniques to Cardiovascular Research. Trends Cardiovasc. Med. 2009, 19, 207–212.
11. D.A.AnnisE.NickbargX.Yang. Affinity Selection–Mass Spectrometry Screening Techniques for Small Molecule Drug Discovery. Curr. Opin. Chem. Biol. 2007, 11, 518–526.
12. N.MaloJ.A.HanleyS.Cerquozzi. Statistical Practice in High-Throughput Screening Data Analysis. Nat. Biotechnol. 2006, 24, 167–175.
13. M.HannB.HudsonX.Lewell. Strategic Pooling of Compounds for High-Throughput Screening. J. Chem. Inf. Comput. Sci. 1999, 39, 897–902.
14. J.C.HermannY.ChenC.Wartchow. Metal Impurities Cause False Positives in High-Throughput Screening Campaigns. ACS Med. Chem. Lett. 2013, 4, 197–200.
15. R.SinkS.GobecS.Pecar. False Positives in the Early Stages of Drug Discovery. Curr. Med. Chem. 2010, 17, 4231–4255.
16. B.Y.FengB.K.ShoichetSynergy and Antagonism of Promiscuous Inhibition in Multiple-Compound Mixtures. J. Med. Chem. 2006, 49, 2151–2154.
17. J.J.DevlinA.LiangL.Trinh. High Capacity Screening of Pooled Compounds: Identification of the Active Compound without Re-Assay of Pool Members. Drug Dev. Res. 1996, 37, 80–85.
18. R.EllsonM.MutzB.Browning. Transfer of Low Nanoliter Volumes between Microplates Using Focused Acoustics—Automation Considerations. J. Lab. Autom. 2003, 8, 29–34.