Development and evaluation of multiplex PCR assays for rapid detection of virulence-associated genes in Arcobacter species

Journal of Microbiological Methods

Volumn 121, Issue , 2016, Pages 59-65

  Whiteduck Léveillée, Jenni ^a   Cloutier, Michel ^a   Topp, Edward ^b   Lapen, David R ^a   Talbot, Guylaine ^c   Villemur, Richard ^d   Khan, Izhar U H ^a  

^a OTTAWA RESEARCH AND DEVELOPMENT CENTRE   (Canada)

^b LONDON RESEARCH AND DEVELOPMENT CENTRE   (Canada)

^c SHERBROOKE RESEARCH AND DEVELOPMENT CENTRE   (Canada)

^d INRS INSTITUT ARMAND FRAPPIER   (Canada)

Author keywords

A. cryaerophilus; A. skirrowii; Arcobacter butzleri; Fecal matter; Multiplex PCR assays; Virulence associated genes

Indexed keywords

BACTERIAL DNA; FIBRONECTIN BINDING PROTEIN; HEMAGGLUTININ; HEMOLYSIN; INVASIN; OUTER MEMBRANE PROTEIN; PHOSPHOLIPASE; VIRULENCE FACTOR; BACTERIAL PROTEIN; FIBRONECTIN; PRIMER DNA;

ARCOBACTER; ARCOBACTER BUTZLERI; ARCOBACTER CRYAEROPHILUS; ARCOBACTER SKIRROWII; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM DETECTION; BACTERIUM ISOLATE; CIAB GENE; CJ1349 GENE; CONTROLLED STUDY; FECES ANALYSIS; HECA GENE; HECB GENE; HUMAN; IRGA GENE; MULTIPLEX POLYMERASE CHAIN REACTION; MVIN GENE; NONHUMAN; PLDA GENE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SINGLEPLEX POLYMERASE CHAIN REACTION; TLYA GENE; VIRULENCE ASSOCIATED GENE; ANIMAL; BACTERIUM IDENTIFICATION; GENETICS; ISOLATION AND PURIFICATION; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PROCEDURES; VIRULENCE;

ANIMALS; ARCOBACTER; BACTERIAL PROTEINS; BACTERIAL TYPING TECHNIQUES; BASE SEQUENCE; DNA PRIMERS; DNA, BACTERIAL; FIBRONECTINS; HUMANS; MULTIPLEX POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIRULENCE; VIRULENCE FACTORS;

EID: 84955307025     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2015.12.017     Document Type: Article

References (39)

1. Abdeldaim G.M.K., Strålin K., Korsgaard J., Blomberg J., Welinder-Olsson C., Herrmann B. Multiplex quantitative PCR for detection of lower respiratory tract infection and meningitis caused by Streptococcus pneumoniae, Haemophilus influenzae and Neisseria meningitides. BMC Microbiol. 2010, 10:310.
2. Andrade G.I., Coura F.M., Santos E.L.S., Ferreira M.G., Galinari G.C.F., Filho E.J.F., de Carvalho A.U., Lage A.P., Heinemann M.B. Identification of virulence factors by multiplex PCR in Escherichia coli isolated from calves in Minas Gerais, Brazil. Trop. Anim. Health Prod. 2012, 44:1783-1790.
3. Antikainen J., Pasanen T., Mero S., Tarkka E., Kirveskari J., Kotila S., Mentula S., Könönen E., Virolainen-Julkunen A.R., Vaara M., Tissari P. Detection of virulence genes of Clostridium difficile by multiplex PCR. APMIS 2009, 117:607-613.
4. Cheng A.F., Yew W.W., Chan E.W., Chin M.L., Hui M.M., Chan R.C. Multiplex PCR amplimer conformation analysis for rapid detection of gyrA mutations in fluoroquinolone-resistant Mycobacterium tuberculosis clinical isolates. Antimicrob. Agents Chemother. 2004, 48:596-601.
5. Collado L., Figueras M.J. Taxonomy, epidemiology, and clinical relevance of the genus Arcobacter. Clin. Microbiol. Rev. 2011, 24:174-192.
6. Collado L., Levican A., Perez J., Figueras M.J. Arcobacter defluvii sp. nov., isolated from sewage samples. Int. J. Syst. Evol. Microbiol. 2011, 61:2155-2161.
7. Dhama K., Rajagunalan S., Chakraborty S., Verma A.K., Kumar A., Tiwari R., Kapoor S. Food-borne pathogens of animal origin diagnosis, prevention, control and their zoonotic significance: a review. Pak. J. Biol. Sci. 2013, 16:1076-1085.
8. Douidah L., De Zutter L., Vandamme P., Houf K. Identification of five human and mammal associated Arcobacter species by a novel multiplex-PCR assay. J. Microbiol. Methods 2010, 80:281-286.
9. Douidah L., de Zuttera L., Baréa J., De Vosb P., Vandammeb P., Vandenbergc O., Van den Abeeled A.-M., Houfa K. Occurrence of putative virulence genes in Arcobacter species isolated from humans and animals. J. Clin. Microbiol. 2012, 50:735-741.
10. Fera M.T., Maugeri T.L., Gugliandolo C., Beninati C., Giannone M., La Camera E., Carbone M. Detection of Arcobacter spp. in the coastal environment of the Mediterranean Sea. Appl. Environ. Microbiol. 2004, 70:1271-1276.
11. Ferrão-Beck L., Cardoso R., Muñoz P.M., de Miguel M.J., Albert D., Ferreira A.C., Marín C.M., Thiébaud M., Jacques I., Grayon M., Zygmunt M.S., Garin-Bastuji B., Blasco J.M., Sá M.I. Development of a multiplex PCR assay for polymorphism analysis of Brucella suis biovars causing brucellosis in swine. Vet. Microbiol. 2006, 115:269-277.
12. Ferreira S., Queiroz J.A., Oleastro M., Domingues F.C. Genotypic and phenotypic features of Arcobacter butzleri pathogenicity. Microb. Pathog. 2014, 76:19-25.
13. Ferreira S., Queiroz J.A., Oleastro M., Domingues F.C. Insights in the pathogenesis and resistance of Arcobacter: a review. Crit. Rev. Microbiol. 2015, 25:1-20.
14. Figueras M.J., Collado L., Levican A., Perez J., Solsona M.J., Yustes C. Arcobacter molluscorum sp. nov., a new species isolated from shellfish. Syst. Appl. Microbiol. 2011, 34:105-159.
15. Franck S.M., Bosworth B.T., Moon H.W. Multiplex PCR for enterotoxigenic, attaching and effacing, and Shiga toxinproducing Escherichia coli strains from calves. J. Clin. Microbiol. 1998, 36:1795-1797.
16. Furian T.Q., Borges K.A., Rocha S.L.S., Rodrigues E.E., do Nascimento V.P., Salle C.T.P., Moraes H.L.S. Detection of virulence-associated genes of Pasteurella multocida isolated from cases of fowl cholera by multiplex-PCR. Pesq. Vet. Bras. 2013, 33:177-182.
17. Gonzalez A., Ferru's M.A. Study of Arcobacter spp. contamination in fresh lettuces detected by different cultural and molecular methods. Int. J. Food Microbiol. 2010, 145:311-314.
18. Gröndahl B., Puppe W., Hoppe A., Kühne I., Weigl J.A.I., Schmitt H.-J. Rapid identification of nine microorganisms causing acute respiratory tract infections by single-tube multiplex reverse transcription-PCR: feasibility study. J. Clin. Microbiol. 1999, 37:11-17.
19. Harmon K.M., Wesley I.V. Identification of Arcobacter isolates by PCR. Lett. Appl. Microbiol. 1996, 23:241-244.
20. Henegariu O., Heerema N.A., Dlouhy S.R., Vance G.H., Vogt P.H. Multiplex PCR: critical parameters and step-by-step protocol. Biotech 1997, 23:504-511.
21. Ho H.T.K., Lipman L.J., Gaastra W. Arcobacter, what is known and unknown about a potential foodborne zoonotic agent. Vet. Microbiol. 2006, 115:1-13.
22. Houf K., Stephan R. Isolation and characterization of the emerging foodborne pathogen Arcobacter from human stool. J. Microbiol. Methods 2007, 68:408-413.
23. Karadas G.1., Sharbati S., Hänel I., Messelhäußer U., Glocker E., Alter T., Gölz G. Presence of virulence genes, adhesion and invasion of Arcobacter butzleri. J. Appl. Microbiol. 2013, 115:583-590.
24. Khan I.U.H., Hill S., Nowak E., Edge T.A. Effect of Incubation temperature on the detection of thermophilic Campylobacter species from freshwater beaches, nearby wastewater effluents, and bird fecal droppings. Appl. Environ. Microbiol. 2013, 79:7639-7645.
25. Kingombe C.I., Huys G., Tonolla M., Albert M.J., Swings J., Peduzzi R., Jemmi T. PCR detection, characterization, and distribution of virulence genes in Aeromonas spp. Appl. Environ. Microbiol. 1999, 65:5293-5302.
26. Kong R.Y.C., Lee S.K.Y., Law T.W.F., Law S.H.W., Wu R.S.S. Rapid detection of six types of bacterial pathogens in marine waters by multiplex PCR. Water Res. 2002, 36:2802-2812.
27. Lehmann L.E., Hunfeld K.-P., Emrich T., Haberhausen G., Wissing H., Hoeft A., Stüber F. A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. Med. Microbiol. Immunol. 2008, 197:313-324.
28. Levican A., Alkeskas A., Günter C., Forsythe S.J., Figueras M.J. The adherence and invasion of human intestinal cells by Arcobacter species and their virulence genotype. Appl. Environ. Microbiol. 2013, 79:4961-4967.
29. McClure J.A., Conly J.M., Lau V., Elsayed S., Louie T., Hutchins W., Zhang K. Novel multiplex PCR assay for detection of the staphylococcal virulence marker Panton-Valentine leukocidin genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci. J. Clin. Microbiol. 2006, 44:1141-1144.
30. Miller W.G., Parker C.T., Rubenfield M., Mendz G.L., Wösten M.M.S.M., Ussery D.W., Stolz J.F., Binnewies T.T., Hallin P.F., Wang G., Malek J.A., Rogosin A., Stanker L.H., Mandrell R.E. The complete genome sequence and analysis of the epsilonproteobacterium Arcobacter butzleri. PLoS One 2007, 2.
31. Sbiti A., Kerch F.E., Sefiani A.A. Analysis of dystrophin gene deletions by multiplex PCR in Moroccan patients. J. Biomed. Biotechnol. 2002, 2:158-160.
32. Tabatabaei M., Askia H.S., Shayegh H., Khoshbakht R. Occurrence of six virulence-associated genes in Arcobacter species isolated from various sources in Shiraz, Southern Iran. Microb. Pathog. 2014, 66:1-4.
33. Thoerner P., Bin Kingombe C.I., Bögli-Stuber K., Bissig-Choisat B., Wassenaar T.M., Frey J., Jemmi T. PCR detection of virulence genes in Yersinia enterocolitica and Yersinia pseudotuberculosis and investigation of virulence gene distribution. Appl. Environ. Microbiol. 2003, 69:1810-1816.
34. US, EPA Guidelines for water reuse United States Environmental Protection Agency, Washington DC 1992.
35. Vandamme P., Falsen E., Rossau R., Hoste B., Segers P., Tytgat R., De Ley J. Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Int. J. Syst. Bacteriol. 1991, 41:88-103.
36. Wang S.M., Khandekar J.D., Kaul K.L., Winchester D.J., Morimoto R.I. A method for the quantitative analysis of human heat shock gene expression using a multiplex RT-PCR assay. Cell Stress Chaperones 1999, 4:153-161.
37. Weltia M., Jatona K., Altweggb M., Sahlia R., Wengera A., Billea J. Development of a multiplex real-time quantitative PCR assay to detect Chlamydia pneumoniae, Legionella pneumophila and Mycoplasma pneumoniae in respiratory tract secretions. Diagn. Microbiol. Infect. Dis. 2003, 2:85-95.
38. Whiteduck-Léveillée K., Whiteduck-Léveillée J., Cloutier M., Tambong J.T., Xu R., Topp E., Arts M.T., Chao J., Adam Z., Lévesque C.A., Lapen D.R., Villemur R., Talbot G., Khan I.U.H. Arcobacter lanthieri sp. nov., isolated from pig and dairy cattle manure. Int. J. Syst. Evol. Microbiol. 2015, 65:2709-2716.
39. Zacharow I., Bystroń J., Wałecka-Zacharska E., Podkowik M., Bania J. Genetic diversity and incidence of virulence-associated genes of Arcobacter butzleri and Arcobacter cryaerophilus isolates from pork, beef, and chicken meat in Poland. BioMed. Res. Int. 2015, 10.1155/2015/956507.