A method for the quantitative analysis of human heat shock gene expression using a multiplex RT-PCR assay

Cell Stress and Chaperones

Volumn 4, Issue 3, 1999, Pages 153-161

  Wang, San Ming ^a,b   Khandekar, Janardan D ^c   Kaul, Karen L ^c   Winchester, David J ^c   Morimoto, Richard I ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

^b UNIVERSITY OF CHICAGO MEDICAL CENTER   (United States)

^c Evanston North western Healthcare   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHAPERONE; HEAT SHOCK PROTEIN; HEAT SHOCK PROTEIN 27; HEAT SHOCK PROTEIN 60; HEAT SHOCK PROTEIN 70; HEAT SHOCK PROTEIN 90; MESSENGER RNA;

ARTICLE; ASSAY; GENE EXPRESSION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TECHNIQUE;

CELL LINE; DNA PRIMERS; GENE EXPRESSION; HEAT-SHOCK PROTEINS; HUMANS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 0032850421     PISSN: 13558145     EISSN: None     Source Type: Journal    
DOI: 10.1379/1466-1268(1999)004<0153:AMFTQA>2.3.CO;2     Document Type: Article

References (26)

1. Bhattacharyya T, Karnezis AN, Murphy SP, Hoang T, Freeman BC, Phillips B, Morimoto RI (1995) Cloning and subcellular localization of human mitochondrial hsp70. J Biol Chem 270: 1705-1710.
2. Dworniczak B and Milault ME (1987) Structure and expression of a human gene coding for a 71 kd heat shock 'cognate' protein. Nucl Acids Res 15: 5181-5197.
3. Fandrey J and Bunn HF (1993) In vivo and in vitro regulation of erythropoietin mRNA: Measurement by competitive polymerase chain reaction. Blood 81: 617-623.
4. Favatier F, Bornman L, Hightower LE, Günther E and Polla BS (1997) Variation in hsp gene expression and Hsp polymorphism: do they contribute to differential disease susceptibility and stress tolerance? Cell Stress Chaperones 2: 141-155.
5. Feige U and Eden WV (1996) Infection, autoimmunity and autoimmune disease. In: Stress-inducible Cellular Responses. U Feige, RI Morimoto, I Yahara and B Polla (eds.) Birkhauser, Basel, pp. 359-373.
6. Ferre F (1992) Quantitative or semi quantitative PCR: Reality versus myth. PCR Meth Applic 2: 1-9.
7. Gilliland G, Perrin S, Blanchard K, Bunn HF (1990) Analysis of cytokine mRNA and DNA: Detection and quantitation by competitive polymerase chain reaction. Proc Nat Acad Sci USA 87: 2725-2729.
8. Gough NM (1988) Rapid and quantitative preparation of cytoplasmic RNA from small numbers of cells. Analyt Biochem 173: 93-95.
9. Hickey E, Brandon SE, Potter R, Stein G, Stein J, Weber LA (1986) Sequence and organization of gene encoding the human 27 kDa heat shock protein. Nucl Acids Res 14: 4127-4145.
10. Hunt C and Morimoto RI (1985) Conserved features of eukaryotic hsp70 genes revealed by comparison with the nucleotide sequence of human hsp70. Proc Nat Acad Sci USA 82: 6455-6459.
11. Jensen LE and Whitehead AS (1998) Competitive reverse transcription polymerase chain reaction for quantifying premRNA and mRNA of major acute phase proteins. Immunol Methods 215: 45-58.
12. Milner CM and Campbell RD (1992) Polymorphic analysis of the three MHC-linked HSP70 genes, Immunogenetics 36: 357-362.
13. Morimoto RI (1998) Regulation of the heat shock transcriptional response: cross talk between a family of heat shock factors, molecular chaperones, and negative regulators. Genes Dev. 12: 3788-3796.
14. Morimoto RI and Santoro MG (1998) Stress-inducible responses and heat shock proteins: New pharmacologic targets for cytoprotection. Nature Biotechnology 16: 833-838.
15. Morimoto RI, Tissieres A, and Georopoulos C (1994) Progress and perpectives on the biology and heat shock proteins and molecular chaperones. In: The Biology of Heat Shock Proteins and Molecular Chaperones. RI Morimoto, A Tissieres and C Georopoulos (eds.) Cold Spring Harbor Press, New York, pp. 1-30.
16. O'Connell J, Goode T and Shanahan F (1998) Quantitative measurement of mRNA expression by competitive RT-PCR. Methods Mol Biol 92: 183-93.
17. Plumier JC, Ross BM, Currie RW, Angelidis CE, Kazlaris H, Kollias G, Pagoulatos GN (1995) Transgenic mice expressing the human heat shock protein 70 have improved post-ischemic myocardial recovery. J Clin Invest 95: 1854-1860.
18. Promega Corporation (1991) Plasmid cloning and transcription in vitro. In: Promega Protocols and Applications Guide (second edition). DE Titus (ed). Promega Corporation, Madison, pp. 58-61.
19. Rebhe NF, Hickman WS, Ley TJ, Stafford DW, Hickman S. (1989) Nucleotide sequence and regulation of a human 90-kDa heat shock protein gene. J Biol Chem 264: 15006-15011.
20. Sambrook J, Fritsch EF, and Maniatis T (1989) Extraction, purification, and analysis of messenger RNA from eukaryotic cells. In: Molecular Cloning: A Laboratory Manual. J Sambrook, EF Fritsch, and T Maniatis (eds). Cold Spring Harbor Laboratory Press, New York, pp 7.2
21. Schwaller J, Pabst T, Bickel M, Borish B, Fey MF, Tobler A (1997) Comparative detection and quantitation of human CDK inhibitor mRNA expression of p15INK4B, p16INK4A, p16beta, p18INK4C, p19INK4D, p21IWAF1, p27KIP1 and p57KIP2 by RT-PCR using a polycompetitive internal standard. Br J Haematol 99: 896-900.
22. Siebert PD and Larrick JW (1992) Competitive PCR. Nature 18(359): 557-558.
23. Sperisen P, Wang SM, Reichenbach P, Nabholz M (1992) A PCR-based assay for reporter gene expression. PCR Meth and Applic 1: 164-170.
24. Ting J and Lee AS (1988) Human gene encoding the 78,000-dalton glucose-regulated protein and its pseudogene: structure, conservation, and regulation. DNA 7: 275-286.
25. Venner TJ, Singh B and Gupta RS (1990) Nucleotide sequence and novel structures of human and Chinese hamster hsp60 (chaperonin) gene families. DNA Cell Biology 9: 545-552.
26. Yamazaki M, Akaogi K, Miwa T, Imai T, Soeda E, Yokoyama K (1989) Nucleotide sequence of a full-length cDNA for 90 kDa heatshock protein from peripheral blood lymphocytes. Nucl Acids Res 17: 7108.