PCR detection of virulence genes in Yersinia enterocolitica and Yersinia pseudotuberculosis and investigation of virulence gene distribution

Applied and Environmental Microbiology

Volumn 69, Issue 3, 2003, Pages 1810-1816

  Thoerner, P ^a   Kingombe, C I Bin ^a   Bogli Stuber K ^a   Bissig Choisat, B ^a   Wassenaar, T M ^c   Frey, J ^b   Jemmi, T ^a  

^a SWISS FEDERAL VETERINARY OFFICE   (Switzerland)

^b UNIVERSITY OF BERN   (Switzerland)

^c Molec Microbiol/Genom Consultants   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; MICROBIOLOGY; PATHOLOGY;

BIOTYPES;

GENES;

CONGO RED;

DETECTION METHOD; MICROBIOLOGY; POLYMERASE CHAIN REACTION; VIRULENCE;

ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GROWTH; BACTERIAL VIRULENCE; BACTERIUM DETECTION; BACTERIUM ISOLATE; BIOASSAY; BIOTYPE; CONTROLLED STUDY; GENOTYPE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RELIABILITY; SEROTYPE; YERSINIA ENTEROCOLITICA; YERSINIA PSEUDOTUBERCULOSIS;

ANIMALS; BACTERIAL PROTEINS; CHROMOSOMES, BACTERIAL; GENOTYPE; HUMANS; PLASMIDS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEROTYPING; VIRULENCE; VIRULENCE FACTORS; YERSINIA ENTEROCOLITICA; YERSINIA INFECTIONS; YERSINIA PSEUDOTUBERCULOSIS;

ANIMALIA; BACTERIA (MICROORGANISMS); NEGIBACTERIA; YERSINIA; YERSINIA ENTEROCOLITICA; YERSINIA PSEUDOTUBERCULOSIS;

EID: 0037335836     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.3.1810-1816.2003     Document Type: Article

References (33)

1. Aleksic, S., and J. Bockemühl. 1999. Yersinia and other enterobacteriaceae, p. 483-496. In P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (ed.), Manual of clinical microbiology, 7th ed. American Society for Microbiology, Washington, D.C.
2. Aulisio, C. C., W. E. Hill, J. T. Stanfield, and R. L. Sellers, Jr. 1983. Evaluation of virulence factor testing and characteristics of pathogenicity in Yersinia enterocolitica. Infect. Immun. 40:330-335.
3. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1999. Current protocols in molecular biology. John Wiley & Sons, New York, N.Y.
4. Bhaduri, S., C. Turner-Jones, M. M. Taylor, and R. V. Lachica. 1990. Simple assay of calcium dependency for virulent plasmid-bearing clones of Yersinia enterocolitica. J. Clin. Microbiol. 28:798-800.
5. Bhaduri, S., C. Turner-Jones, and R. V. Lachica. 1991. Convenient agarose medium for simultaneous determination of the low-calcium response and Congo red binding by virulent strains of Yersinia enterocolitica. J. Clin. Microbiol. 29:2341-2344.
6. Bhaduri, S., and B. Cottrell. 1997. Direct detection and isolation of plasmid-bearing virulent serotypes of Yersinia enterocolitica from various foods. Appl. Environ. Microbiol. 63:4952-4955.
7. Blais, B. W., and L. M. Phillippe. 1995. Comparative analysis of yadA and ail polymerase chain reaction methods for virulent Yersinia enterocolitica. Food Control 6:211-214.
8. Bölin, I., A. Forsberg, L. Norlander, M. Skurnik, and H. Wolf-Watz. 1988. Identification and mapping of the temperature-inducible plasmid-encoded proteins of Yersinia spp. Infect. Immun. 56:343-348.
9. Bottone, E. J. 1997. Yersinia enterocolitica: the charisma continues. Clin. Microbiol. Rev. 10:257-276.
10. Burnens, A. P., A. Frey, and J. Nicolet. 1996. Association between clinical presentation, biogroups and virulence attributes of Yersinia enterocolitica strains in human diarrhoeal disease. Epidemiol. Infect. 116:27-34.
11. Cornelis, G., C. Sluiters, C. L. de Rouvroit, and T. Michiels. 1989. Homology between VirF, the transcriptional activator of the Yersinia virulence regulon, and AraC, the Escherichia coli arabinose operon regulator. J. Bacteriol. 171:254-262.
12. Delor, I., A. Kaeckenbeeck, G. Wauters, and G. R. Cornelis. 1990. Nucleotide sequence ofyst, the Yersinia enterocolitica gene encoding the heat-stable enterotoxin, and prevalence of the gene among pathogenic and nonpathogenic yersiniae. Infect. Immun. 58:2983-2988.
13. Farmer, J. J., G. P. Carter, V. L. Miller, S. Falkow, and I. K. Wachsmuth. 1992. Pyrazinamidase, CR-MOX agar, salicin fermentation-esculin hydrolysis, and D-xylose fermentation for identifying pathogenic serotypes of Yersinia enterocolitica. J. Clin. Microbiol. 30:2589-2594.
14. Grant, T., V. Bennett-Wood, and R. M. Robins-Browne. 1998. Identification of virulence-associated characteristics in clinical isolates of Yersinia enterocolitica lacking classical virulence markers. Infect. Immun. 66:1113-1120.
15. Isberg, R. R., D. L. Voorhis, and S. Falkow. 1987. Identification of invasin: a protein that allows enteric bacteria to penetrate cultured mammalian cells. Cell 50:769-778.
16. Kandolo, K., and G. Wauters. 1985. Pyrazinamidase activity in Yersinia enterocolitica and related organisms. J. Clin. Microbiol. 21:980-982.
17. Koeppel, E., R. Meyer, J. Luethy, and U. Candrian. 1993. Recognition of pathogenic Yersinia enterocolitica by crystal violet binding and polymerase chain reaction. Lett. Appl. Microbiol. 17:231-234.
18. Kuhnert, P., J. Hacker, I. Mühldorfer, A. P. Burnens, J. Nicolet, and J. Frey. 1997. Detection system for Escherichia coli-specific virulence genes: absence of virulence determinants in B and C strains. Appl. Environ. Microbiol. 63:703-709.
19. Kwaga, J., J. O. Iversen, and V. Misra. 1992. Detection of pathogenic Yersinia enterocolitica by polymerase chain reaction and digoxigenin-labeled polynucleotide probes. J. Clin. Microbiol. 30:2668-2673.
20. Lewin, A., E. Strauch, S. Hertwig, B. Hoffmann, H. Nattermann, and B. Appel. 1996. Comparison of plasmids of strains of Yersinia enterocolitica biovar 1A with the virulence plasmid of a pathogenic Y. enterocolitica strain. Zentbl. Bakteriol. 285:52-63.
21. Lindsay, J. A. 1997. Chronic sequelae of foodborne disease. Emerg. Infect. Dis. 3:443-452.
22. Miller, V. L., J. J. Farmer III, W. E. Hill, and S. Falkow. 1989. The ail locus is found uniquely in Yersinia enterocolitica serotypes commonly associated with disease. Infect. Immun. 57:121-131.
23. Miller, V. L., J. B. Bliska, and S. Falkow. 1990. Nucleotide sequence of the Yersinia enterocolitica ail gene and characterization of the Ail protein product. J. Bacteriol. 172:1062-1069.
24. Morris, J. G., Jr., V. Prado, C. Ferreccio, R. M. Robins-Browne, A.-M. Bordun, M. Cayazzo, B. A. Kay, and M. M. Levine. 1991. Yersinia enterocolitica isolated from two cohorts of young children in Santiago, Chile: incidence of and lack of correlation between illness and proposed virulence factors. J. Clin. Microbiol. 29:2784-2788.
25. Pitcher, D., N. Saunders, and R. Owen. 1989. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett. Appl. Microbiol. 8:151-156.
26. Prpic, J. K., R. M. Robins-Browne, and R. B. Davey. 1983. Differentiation between virulent and avirulent Yersinia enterocolitica isolates by using Congo red agar. J. Clin. Microbiol. 18:486-490.
27. Ramamurthy, T., K. I. Yoshino, X. Huang, G. Balakrish Nair, E. Carniel, T. Maruyama, H. Fukushima, and T. Takeda. 1997. The novel heat-stable enterotoxin subtype gene (ystB) of Yersinia enterocolitica: nucleotide sequence and distribution of the yst genes. Microb. Pathog. 23:189-200.
28. Riley, G., and S. Toma. 1989. Detection of pathogenic Yersinia enterocolitica by using Congo red-magnesium oxalate agar medium. J. Clin. Microbiol. 27:213-214.
29. Robins-Browne, R. M., T. Takeda, A. Fasano, A. M. Bordun, S. Dohi, H. Kasuga, G. Fang, V. Prado, R. L. Guerrant, et al. 1993. Assessment of enterotoxin production by Yersinia enterocolitica and identification of a novel heat-stable enterotoxin produced by a noninvasive Y. enterocolitica strain isolated from clinical material. Infect. Immun. 61:764-767.
30. Skurnik, M., and H. Wolf-Watz. 1989. Analysis of the yopA gene encoding the Yop1 virulence determinants of Yersinia spp. Mol. Microbiol. 3:517-529.
31. 2+ response virulence regulon of human-pathogenic yersiniae. Microb. Pathog. 10:87-91.
32. Wauters, G., K. Kandolo, and M. Janssens. 1987. Revised biogrouping scheme of Yersinia enterocolitica. Contrib. Microbiol. Immunol. 9:14-21.
33. Yoshino, K., H. Xiaozhe, M. Miyachi, Y. Hong, T. Takao, H. Nakao, T. Takeda, and Y. Shimonishi. 1994. Amino acid sequence of a novel heatstable enterotoxin produced by ayst gene-negative strain of Yersinia enterocolitica. Lett. Pept. Sci. 1:95-105.