Process limitations of a whole-cell P450 catalyzed reaction using a CYP153A-CPR fusion construct expressed in Escherichia coli

Applied Microbiology and Biotechnology

Volumn 100, Issue 3, 2016, Pages 1197-1208

  Lundemo, M T ^a   Notonier, S ^b   Striedner, G ^c   Hauer, B ^b   Woodley, J M ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b UNIVERSITY OF STUTTGART   (Germany)

^c UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

Bottleneck identification; Cytochrome P450 monooxygenases; Inhibition; Process development; Whole cell catalysis

Indexed keywords

BACTERIOLOGY; CATALYSIS; CATALYST ACTIVITY; CELLS; CYTOLOGY; ENZYME INHIBITION; ESCHERICHIA COLI; FATTY ACIDS; HYDROXYLATION;

BACILLUS MEGATERIUM; BOTTLENECK IDENTIFICATIONS; CATALYZED REACTIONS; CYTOCHROME P450 MONOOXYGENASES; HYDROXYLATED FATTY ACIDS; PROCESS DEVELOPMENT; VOLUMETRIC PRODUCTIVITY; WHOLE CELL;

BIOCATALYSTS;

ACETONE; COSMETIC; CYTOCHROME P450; CYTOCHROME P450 153A; CYTOCHROME P450 BM3; DICARBOXYLIC ACID; DIMETHYL SULFOXIDE; FATTY ACID; FRAGRANCE; HEME; LAURIC ACID; LONG CHAIN FATTY ACID; OMEGA HYDROXYLATED FATTY ACID; OXIDOREDUCTASE; POLYMER; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; HYBRID PROTEIN;

BACTERIUM; BIOTECHNOLOGY; CATALYSIS; CELLS AND CELL COMPONENTS; CYTOCHROME; ENZYME ACTIVITY; GENE EXPRESSION; HYDROCARBON; INHIBITION; MICROBIOLOGY; MOLECULAR ANALYSIS; POPULATION BOTTLENECK;

ARTICLE; BACILLUS MEGATERIUM; BACTERIAL STRAIN; BATCH FERMENTATION; BATCH PROCESS; BIOCATALYST; BIOTRANSFORMATION; CARBON SOURCE; CATALYSIS; CELL DENSITY; CELL MEMBRANE; CONTROLLED STUDY; ENZYME INACTIVATION; ESCHERICHIA COLI; FREEZE THAWING; HOST CELL; MARINOBACTER HYDROCARBONOCLASTICUS; NEGATIVE FEEDBACK; NONHUMAN; OXIDATION; PROCESS DEVELOPMENT; PROTEIN EXPRESSION; STIRRED REACTOR; SUBSTRATE CONCENTRATION; ULTRASOUND; WHOLE CELL; CHEMISTRY; ENZYMOLOGY; GENE EXPRESSION; GENETICS; KINETICS; MARINOBACTER; METABOLISM; PROTEIN TERTIARY STRUCTURE;

BACILLUS MEGATERIUM; ESCHERICHIA COLI; MARINOBACTER HYDROCARBONOCLASTICUS;

BACILLUS MEGATERIUM; BACTERIAL PROTEINS; CATALYSIS; CYTOCHROME P-450 ENZYME SYSTEM; ESCHERICHIA COLI; FATTY ACIDS; GENE EXPRESSION; KINETICS; MARINOBACTER; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS;

EID: 84955207211     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-015-6999-x     Document Type: Article

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