Process development for the production of 15β-hydroxycyproterone acetate using Bacillus megaterium expressing CYP106A2 as whole-cell biocatalyst

Microbial Cell Factories

Volumn 14, Issue 1, 2015, Pages

  Kiss, Flora M ^a   Lundemo, Marie T ^b   Zapp, Josef ^a   Woodley, John M ^b   Bernhardt, Rita ^a  

^a SAARLAND UNIVERSITY   (Germany)

^b TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

Bacillus megaterium; Bioreactor; Cyclodextrin; CYP106A2; Cyproterone acetate; Cytochrome P450; Steroid hydroxylase; Whole cell biocatalyst

Indexed keywords

15BETA HYDROXYCYPROTERONE ACETATE; 2 HYDROXYPROPYL BETA CYCLODEXTRIN; CYPROTERONE ACETATE; CYPROTERONE DERIVATIVE; CYTOCHROME P450; CYTOCHROME P450 106A2; TESTOSTERONE DERIVATIVE; UNCLASSIFIED DRUG; 15BETA-HYDROXYLASE CYP106A2, BACILLUS MEGATERIUM; 2-HYDROXYPROPYL-BETA-CYCLODEXTRIN; BACTERIAL PROTEIN; BETA CYCLODEXTRIN DERIVATIVE; RECOMBINANT PROTEIN;

ARTICLE; BACILLUS MEGATERIUM; BIOCATALYST; BIOREACTOR; BIOTRANSFORMATION; CONTROLLED STUDY; ENZYME SUBSTRATE; ENZYME SYNTHESIS; FERMENTATION; IN VITRO STUDY; IN VIVO STUDY; LARGE SCALE PRODUCTION; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROCESS DEVELOPMENT; PROTEIN EXPRESSION; SCALE UP; STRUCTURE ANALYSIS; SYNTHESIS; WHOLE CELL; BIOCATALYSIS; BIOSYNTHESIS; CHEMISTRY; ENZYME SPECIFICITY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; STEREOISOMERISM;

BACILLUS MEGATERIUM; BACTERIAL PROTEINS; BETA-CYCLODEXTRINS; BIOCATALYSIS; CYPROTERONE ACETATE; CYTOCHROME P-450 ENZYME SYSTEM; HUMANS; KINETICS; MAGNETIC RESONANCE SPECTROSCOPY; RECOMBINANT PROTEINS; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

EID: 84928730984     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-015-0210-z     Document Type: Article

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