Development of a whole-cell biocatalyst co-expressing P450 monooxygenase and glucose dehydrogenase for synthesis of epoxyhexane

Applied Microbiology and Biotechnology

Volumn 95, Issue 2, 2012, Pages 357-367

  Siriphongphaew, Akasit ^a   Pisnupong, Pimpaya ^a,b   Wongkongkatep, Jirarut ^a   Inprakhon, Pranee ^a   Vangnai, Alisa S ^c   Honda, Kohsuke ^d   Ohtake, Hisao ^d   Kato, Junichi ^e   Ogawa, Jun ^f   Shimizu, Sakayu ^f   Urlacher, Vlada B ^g   Schmid, Rolf D ^h   Pongtharangkul, Thunyarat ^a,b  

^a Mahidol University ^*  (Thailand)

^b Thailand Ministry of Science and Technology   (Thailand)

^c CHULALONGKORN UNIVERSITY   (Thailand)

^d OSAKA UNIVERSITY   (Japan)

^e HIROSHIMA UNIVERSITY   (Japan)

^f KYOTO UNIVERSITY   (Japan)

^g HEINRICH HEINE UNIVERSITY   (Germany)

^h UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Bacillus subtilis; Epoxidation; P450 BM3; Whole cell biocatalyst

Indexed keywords

1-HEXENE; BACILLUS SUBTILIS; CELL SURFACES; CHEMICAL CATALYST; CO-EXPRESSION; E. COLI; GLUCOSE DEHYDROGENASE; HETEROLOGOUS EXPRESSION; MONOOXYGENASES; OXYGENASES; P450 BM3; PRODUCT CONCENTRATION; REACTION CONDITIONS; SUBSTRATE CONCENTRATIONS; SUBTILIS; SYSTEMATIC OPTIMIZATION; TERMINAL ALKENES; WHOLE CELL BIOCATALYSTS; WILD TYPES;

CELL MEMBRANES; EPOXIDATION; ESCHERICHIA COLI; GLUCOSE; OLEFINS; OPTIMIZATION; ORGANIC SOLVENTS; SUBSTRATES;

CYTOLOGY;

1 HEXENE; CYTOCHROME P450; EPOXYHEXANE; GLUCOSE DEHYDROGENASE; HEXANE; PLASMID DNA; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE;

CATALYSIS; CATALYST; COLIFORM BACTERIUM; ENZYME ACTIVITY; MEMBRANE; OPTIMIZATION; SUBSTRATE;

ARTICLE; BACILLUS SUBTILIS; BIOCATALYST; BIOSYNTHESIS; ENZYME ASSAY; EPOXIDATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GAS CHROMATOGRAPHY; NONHUMAN; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; TOXICITY TESTING;

BACILLUS SUBTILIS; CYTOCHROME P-450 ENZYME SYSTEM; DNA, BACTERIAL; DNA, RIBOSOMAL; EPOXY COMPOUNDS; GLUCOSE 1-DEHYDROGENASE; HEXANES; MOLECULAR SEQUENCE DATA; NADP; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA;

BACILLUS SUBTILIS; ESCHERICHIA COLI;

EID: 84864713846     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4039-7     Document Type: Article

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