Cartilage graft engineering by co-culturing primary human articular chondrocytes with human bone marrow stromal cells

Journal of Tissue Engineering and Regenerative Medicine

Volumn 9, Issue 12, 2015, Pages 1394-1403

  Sabatino, Maria Antonietta ^a,b   Santoro, Rosaria ^a   Gueven, Sinan ^a   Jaquiery, Claude ^a   Wendt, David James ^a   Martin, Ivan ^a   Moretti, Matteo ^c   Barbero, Andrea ^a  

^a UNIVERSITY HOSPITAL BASEL   (Switzerland)

^b IRCCS POLICLINICO SAN DONATO   (Italy)

^c IRCCS ISTITUTO ORTOPEDICO GALEAZZI   (Italy)

Author keywords

Bioreactor culture; Chondrocytes, cartilage engineering; Mesenchymal stem cells; Nude mice

Indexed keywords

BIOREACTORS; BODY FLUIDS; BONE; CARTILAGE; CELL ENGINEERING; COLLAGEN; FLOWCHARTING; HISTOLOGY; MAMMALS; SCAFFOLDS (BIOLOGY); STEM CELLS;

ARTICULAR CHONDROCYTES; BIOREACTOR CULTURES; BONE MARROW; CARTILAGE ENGINEERING; CHONDROCYTE, CARTILAGE ENGINEERING; CHONDROCYTES; CO-CULTURES; IN-VITRO; MESENCHYMAL STEM CELL; NUDE MICE;

CELL CULTURE;

COLLAGEN TYPE 10; COLLAGEN TYPE 2; GLYCOSAMINOGLYCAN;

ADULT; AGED; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BONE MARROW STROMA CELL; CARBOHYDRATE ANALYSIS; CARTILAGE CELL; CARTILAGE GRAFT; CELLS BY BODY ANATOMY; CHONDROGENESIS; COCULTURE; CONTROLLED STUDY; FEMALE; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; IN VIVO STUDY; MALE; MATERIALS TESTING; MIDDLE AGED; MOUSE; NONHUMAN; PRIORITY JOURNAL; TISSUE ENGINEERING; TISSUE REPAIR; TISSUE SCAFFOLD; ANIMAL; ARTICULAR CARTILAGE; BONE MARROW CELL; CHONDROCYTE; CYTOLOGY; METABOLISM; NUDE MOUSE; PROCEDURES; STROMA CELL; TRANSPLANTATION; XENOGRAFT;

ADULT; AGED; ANIMALS; BONE MARROW CELLS; CARTILAGE, ARTICULAR; CHONDROCYTES; COCULTURE TECHNIQUES; FEMALE; HETEROGRAFTS; HUMANS; MALE; MICE; MICE, NUDE; MIDDLE AGED; STROMAL CELLS; TISSUE ENGINEERING;

EID: 84955170514     PISSN: 19326254     EISSN: 19327005     Source Type: Journal    
DOI: 10.1002/term.1661     Document Type: Article

References (30)

1. Acharya C, Adesida A, Zajac P, et al. 2012; Enhanced chondrocyte proliferation and mesenchymal stromal cells chondrogenesis in coculture pellets mediate improved cartilage formation. J Cell Physiol 227: 88-97.
2. Aung A, Gupta G, Majid G, et al. 2011; Osteoarthritic chondrocyte-secreted morphogens induce chondrogenic differentiation of human mesenchymal stem cells. Arthrit Rheum 63: 148-158.
3. Barbero A, Grogan SP, Schaefer D, et al. 2004; Age related changes in human articular chondrocyte yield, proliferation and post-expansion chondrogenic capacity. Osteoarthrit Cartilage 12: 476-484.
4. Benya PD, Shaffer JD. 1982; Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell 30: 215-224.
5. Bian L, Zhai DY, Mauck RL, et al. 2011; Coculture of human mesenchymal stem cells and articular chondrocytes reduces hypertrophy and enhances functional properties of engineered cartilage. Tissue Eng Part A 17: 1137-1145.
6. Brittberg M, Lindahl A, Nilsson A, et al. 1994; Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med 33: 889-895.
7. Chai DH, Arner EC, Griggs DW, et al. 2010; αv and β1 integrins regulate dynamic compression-induced proteoglycan synthesis in 3D gel culture by distinct complementary pathways. Osteoarthrit Cartilage 18: 249-256.
8. Darling EM, Athanasiou KA. 2005; Rapid phenotypic changes in passaged articular chondrocyte subpopulations. J Orthop Res 23: 425-432.
9. Dell'Accio F, De Bari C, Luyten FP. 2001; Molecular markers predictive of the capacity of expanded human articular chondrocytes to form stable cartilage in vivo. Arthritis Rheum 44: 1608-1619.
10. Diaz-Romero J, Gaillard JP, Grogan SP, et al. 2005; Immunophenotypic analysis of human articular chondrocytes: changes in surface markers associated with cell expansion in monolayer culture. J Cell Physiol 202: 731-742.
11. Dickhut A, Pelttari K, Janicki P, et al. 2009; Calcification or dedifferentiation: requirement to lock mesenchymal stem cells in a desired differentiation stage. J Cell Physio 219: 219-226.
12. Farndale RW, Buttle DJ, Barrett AJ. 1986; Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. Biochim Biophys Acta 883: 173-177.
13. Fischer J, Dickhut A, Rickert M, et al. 2010; Articular chondrocytes secrete PTHrP and inhibit hypertrophy of mesenchymal stem cells in coculture during chondrogenesis. Arthritis Rheum 62: 2696-2706.
14. Francioli SE, Candrian C, Martin K, et al. 2010; Effect of three-dimensional expansion and cell-seeding density on the cartilage-forming capacity of human articular chondrocytes in type II collagen sponges. J Biomed Mater Res A 95: 924-931.
15. Giovannini S, Diaz-Romero J, Aigner T, et al. 2010; Micromass co-culture of human articular chondrocytes and human bone marrow mesenchymal stem cells to investigate stable neocartilage tissue formation in vitro. Eur Cell Mater 20: 245-259.
16. Hildner F, Concaro S, Peterbauer A, et al. 2009; Human adipose-derived stem cells contribute to chondrogenesis in coculture with human articular chondrocytes. Tissue Eng Part A 15: 3961-3969.
17. Jin M, Frank EH, Quinn TM, et al. 2001; Tissue shear deformation stimulates proteoglycan and protein biosynthesis in bovine cartilage explants. Arch Biochem Biophys 395: 41-48.
18. Kisiday JD, Jin M, DiMicco MA, et al. 2004; Effects of dynamic compressive loading on chondrocyte biosynthesis in self-assembling peptide scaffolds. J Biomech 37: 595-604.
19. Liu X, Sun H, Yan D, et al. 2010; In vivo ectopic chondrogenesis of BMSCs directed by mature chondrocytes. Biomaterials 31: 9406-9414.
20. Miao C, Mu S, Duan P, et al. 2009; Effects of chondrogenic microenvironment on construction of cartilage tissues using marrow stromal cells in vitro. Artif Cells Blood Substit Biotechnol 37: 214-221.
21. Moretti M, Wendt W, Dickinson SC, et al. 2005; Effects of in vitro preculture on in vivo development of human engineered cartilage in an ectopic model. Tissue Eng 11: 1421-1428.
22. Peterson L, Minas T, Brittberg M, et al. 2000; Two-to-nine-year outcome after autologous chondrocyte transplantation of the knee. Clin Orthop Relat Res 374: 212-234.
23. Santoro R, Olivares AL, Brans G, et al. 2010; Bioreactor based engineering of large-scale human cartilage grafts for joint resurfacing. Biomaterials 31: 8946-8952.
24. Saris DB, Vanlauwe J, Victor J, et al. 2008; Characterized chondrocyte implantation results in better structural repair when treating symptomatic cartilage defects of the knee in a randomized controlled trial vs microfracture. Am J Sports Med 36: 235-246.
25. Tsuchiya K, Chen G, Ushida T, et al. 2004; The effect of coculture of chondrocytes with mesenchymal stem cells on their cartilaginous phenotype in vitro. Mater Sci Eng C 24: 391-396.
26. Wendt D, Stroebel S, Jakob M, et al. 2006; Uniform tissues engineered by seeding and culturing cells in 3D scaffolds under perfusion at defined oxygen tensions. Biorheology 43: 841-848.
27. Wendt D, Marsano A, Jakob M, et al. 2003; Oscillating perfusion of cell suspensions through three-dimensional scaffolds enhances cell seeding efficiency and uniformity. Biotechnol Bioeng 84: 205-214.
28. Wu L, Leijten JC, Georgi N, et al. 2011; Trophic effects of mesenchymal stem cells increase chondrocyte proliferation and matrix formation. Tissue Eng Part A 17: 1425-1436.
29. Wu L, Prins HJ, Helder MN, et al. 2012; Trophic effects of mesenchymal stem cells in chondrocyte co-cultures are independent of culture conditions and cell sources. Tissue Eng Part A 18: 1542-1551.
30. Yang HN, Park JS, Na K, et al. 2009; The use of green fluorescence gene (GFP)-modified rabbit mesenchymal stem cells (rMSCs) co-cultured with chondrocytes in hydrogel constructs to reveal the chondrogenesis of MSCs. Biomaterials 30: 6374-6385.